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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr660
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9h after fertilization: strong staining in intestinal and hypodermal nuclei; Weak neuronal staining. Early L1: staining in nuclei of most postembryonic blast cells. Strong staining in nuclei of hypodermic blast cells H1, H2, V1-V6, T and all intestinal (E) cells. Weak staining nuclei of neuroblasts Q1 and Q2, mesoblast M cells and P cells. 9h after fertilization: strong staining in intestinal and hypodermal nuclei; Weak neuronal staining. Early L1: staining in nuclei of most postembryonic blast cells. Strong staining in nuclei of hypodermic blast cells H1, H2, V1-V6, T and all intestinal (E) cells. weak staining nuclei of neuroblasts Q1 and Q2, mesoblast M cells and P cells. Adult: staining observed in the mature oocyte nuclei of hermaphrodites, at meiotic prophase I when the chromosomes are condensed. (Possible artifact, detected in lin-14 loss-of-function mutant strains n536n540, n355n726). In embryo, first observed in embryo at 7h after fertilization (half way through embryogenesis). Strong staining in intestinal and hypodermal nuclei. L3: Pn.p stains weakly before division (staining fades by L4). Occasional weak staining of hypodermal, intestinal and neuronal nuclei and cytoplasm at L2 and L3. Late L1: staining of all nuclei except for neuronal nuclei is weaker. More neuron of the nerve ring and posterior ganglion stain than in earlier stages. Intestinal and hypodermal cell lineages stain strongest at mid to late L1 (Fade entirely by L2) similarly with many of the neuronal cells. Mid L1: staining in nuclei of hypodermic blast cells H1, H2, V1-V6 and T. The nuclei of intestinal (E) cells also stain. Weak staining in nuclei of P cells (staining fades before migration into ventral cord). Strong staining in nuclei of embryo-derived nuclei in hypodermal syncytial cell hyp7, ABarpppapa, ABplaapppp, Cpaaaa, Cpaapa, Cpaapp, Cpapaa, terminally differentiated nuclei from embryonic body muscle also stain for lin-14. Staining observed in nuclei of neuronal cells BDU, ALM, and CAN. All embryonic generated ventral cord neurons and some neurons of the nerve ring and posterior ganglion stain for lin-14. |
lin-14 is localized to the nuclei. |
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Lineage expression: H, V, T descandents. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr661
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lin-14 protein is first observed in embryos at ~7 hours after fertilization where most intense staining is seen in intestinal and hypodermal nuclei. ~9 h after fertilization, additional weak staining is observed. lin-14 protein is expressed at high level in the nuclei of most of the post-embryonic blast cells. Intense nuclear staining was observed in the hypodermal blast cells H1, H2, V1-V6 and T and in all of the intestinal (E) cells and weaker staining was observed in both neuroblasts Q1 and Q2, in the mesoblast M cell and in P cells (P1/2, P3/4 and P5/6). During L1, staining is seen in the progeny of the hypodermal blast cells H1, H2 V1-V6 and T and in all of the intestinal (E) cells. Staining in P-cell nuclei fades before migration into the ventral nerve cord but reappears later in some of their progeny cells. The embryo-derived nuclei in the hypodermal syncytial cell hyp7, ABarpppapa, ABplaapppp, Cpaaaa, Cpaapa, Cpaapp, Cpapaa, all stain for the lin-14 protein during the L1 stage. Terminally differentiated nuclei from embryonic body muscle also accumulate the lin-14 protein. Nuclei of many but not all neuronal cells stain with the antibody (e.g. BDU, ALM, CAN but not HSN). All of the embryonically generated ventral cord neurons and some but not all of the neurons of the nerve ring and the posterior ganglion accumulate the lin-14 protein in their nuclei during the L1 stage. Late L1 stage, staining is seen in all nuclei except in the neuronal nuclei staining is much weaker. In addition, more neurons of the nerve ring and posterior ganglion stain than at the earlier stages. Thus, in the hypodermal and intestinal cell lineages, lin-14 protein level peaks during early L1 and fade entirely by L2. In the many neuronal cells, lin-14 protein peak during mid to late L1 and fade by L2. Pn.p accumulates lin-14 protein at the L3 stage, although, very weak staining is seen before the Pn.p cells divide. This staining fades by early L4, In occasional L2 and L3 stage animals, weak staining is observed in nuclei and cytoplasm of hypodermal, neuronal and intestinal cells. Patches of staining in hypodermal or intestinal nuclei is only rarely observed in very old adults. In most adults, staining reappears only in the mature oocyte nuclei of hermaphrodites at meiotic prophase I when the chromosomes are condensed. The oocyte nuclear staining disappears after fertilization. Quantitation of immunoblots show that the level of lin-14 protein relative to a pharyngeal myosin control decreases >= 25-fold from L1 to L2. |
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Other strain-- UL123 |
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Expr103
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This strain exhibits strong expression in the embryo. Expression is first seen in the 50-80 cell embryo and extends through to adulthood. It appears that most of the AB cells in the embryo stain, and what appears to be the cells of the C lineage. Some embryos exhibit staining in the two rows of nuclei that are the E lineage. All embryonic staining is very intense, and it spreads to the cytoplasm giving blue embryos, therefore obscuring the DAPI staining, making it difficult to count the number of cells in the embryos as each component begins expressing. This intense staining fades as the embryo ages, sometimes leaving blue comma stage embryos with no distinct nuclei staining. Hypodermal expression is seen in the 3 fold stage of embryogenesis and in young larvae which most probably are C-derived hyp-7 nuclei. Expression weakens as the worm gets older and is much less frequently expressed in adults. Some adults do show staining in the anterior hypodermal nuclei (hyp-3, hyp-4) and in the anterior hypodermal seam cells, also some nuclei stain in the tail. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr706
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NHR-2 is detected in the nuclei of embryos as early as 2-cell stage. The protein is present in every nucleus until the 16-20 cell stage then no longer detected in germline precursor P4 and its sister D but at this point expression in other cells increase. No staining during or just after mitosis. 28-cell stage: Staining in E and MS descendants, variable expression generally weak particularly in E cells. Staining in ABplp and ABpr descendants also variable but can be quite strong. The other 10 AB cells and 4 C cells exhibit reproducible strong expression. 51-cell stage: No expression in descendants of E. Staining in C cells, many AB cells and some MS cells (particularly those in anterior and dorsal positions). As embryogenesis progresses NHR-2 expression is restricted to anterior and dorsal regions of embryo. 250 cell stage: Nuclei staining include (but not limited to) Cp descendants contributing to hyp7 synctium, many but not all AB descendants. NHR-2 last detected in one or a few nuclei in vicinity of excretory cell before expression ceases at early comma stage. |
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Three methods, lacZ, gfp, antibody staining results all mixed together. Lots of unextracted cell objects buried in pattern text. |
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Expr841
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PAL-1 produced from zygotic transcripts is seen initially in C and D lineage cells that also expressed maternally derived PAL-1. As gastrulation begins, expression is seen in only Ca and Cp and then in their daughters, of which 2 are hypodermoblasts (Caa and Cpa) and 2 are myoblasts (Cap and Cpp). The GFP reporter is first detected at the late 2C-cell stage and then more strongly in the 4 daughters. At about 100 cells, expression is also detected in the 2 D-lineage myoblasts. Thereafter, PAL-1 continues to be detected in all C and D descendants until the end of gastrulation at about 350 cells. At about 180 cells (midgastrulation), the C hypodermal precursors, which express more strongly than the muscle precursors, form a characteristic double row on each side of the dorsal midline in the posterior. Thereafter, PAL-1 decreases in these cells and is no longer detectable with antibody after 350 500 cells. At about 250 cells, expression is detected in two AB cells that border the posterior left edge of the mesectodermal cell layer that is closing the ventral gastrulation cleft (ABplpappp and ABplppppp) and slightly later in the right homolog of one of them (ABprppppp). The daughters and granddaughters of these cells, generated after the cleft closes, continue to express strongly along the ventral midline until about the time of hatching. Beginning at about 360 cells, as morphogenesis begins, weak transient expression is detected in the posterior ectodermal P cells and occasionally in posterior V cells as both groups move ventrally. During this period the V cells become the lateral seam cells, and the P cells undergo their terminal embryonic divisions as they complete hypodermal enclosure of the embryo. Meanwhile, in the interior, pal-1 expression, detectable both with antibody and with reporter constructs, appears at about 350 cells in 2 Ea descendents near the middle of the gut primordium (the int5 pair) and in 2 anteriorly located MS descendants which migrate to the posterior and become the mesoblast M and the right intestinal muscle (mu intR). During early morphogenesis as the embryo develops through the comma stage and begins to elongate, all the pal-1-expressing cells (approximately 50) are located in the posterior ventral region, except for the 2 midgut cells which lie more dorsally. The descendants of ABpl/rppppp, as well as mu intR, move into the elongating tail and participate in formation of the rectal and associated intestinal muscles, as well as the ventral tail hypodermis. Expression diminishes during elongation and by hatching is detectable only in the 2 gut cells, M, mu intR, and 10 cells descended from ABpl/rppppp. |
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Transgenic Marker: rol-6(su1006). |
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Expr517
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At approximately 260 min after the first cleavage, reporter gene expression is now also detected in nuclei on the dorsal surface of the embryo, corresponding to the position of the major hypodermal cells, and in six nuclei on each ventrolateral aspect of the embryo, corresponding to the position of the ventral hypodermal cells (P cells). Expression was first seen in granddaughters of C.paa and C.aaa about 240 min. after first cleavage. About 260 min. after first cleavage, expression was seen in dorsal and ventral hypodermal cells. Between comma-stage and 3-fold stage, expression was seen in dorsal and ventral hypodermal cells, and in tail and head hypodermis. After the 3-fold stage, expression becomes reduced, but is maintained a low levels in larvae and adults. After the 3-fold stage through adulthood, a digestive-tract component is expressed: probably vpi3D and vpi3V in the pharyngeal intestinal valve, virL and virR in intestinal rectal valve, and rect D, rect VL, rect VR. |
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Expr10221
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10307
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10526
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Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10422
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Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10230
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10214
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10198
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ten-1a::gfp promoter reporter shows expression in the same epidermal blastomeres as lat-1::gfp (Expr10197) and additional lineages (ABaraax, MSapx, MSppx; data not shown) except one (Cpaaaa). |
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Expr10351
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10352
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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