WormMine: ExpressionPattern Expr4327

• Pattern: Transgenic mgEx674[mir-84::gfp] animals expressed GFP in the lateral hypodermal seam cells and other cells. The mir-84::gfp reporter was expressed in seam cells during early larval stages in some transgenic animals, although expression was more prevalent in L3-stage and older animals.
• Primary Identifier: Expr4327
• Remark: No obvious difference in expression was observed between this particular mir-84::gfp fusion gene and one in which 8.1 kb of sequence upstream of mir-84 was fused to yellow fluorescent protein, kindly provided by A. Yoo and I. Greenwald.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522
The components of the gonad that are separate from the germline proper. In hermaphrodite, these include five tissues which are all derived from the somatic primordium : the distal tip cells, the gonadal sheath, the spermatheca, the spermatheca-uterine valve (sp-ut) and the uterus.	somatic gonad	somatic germline	WBbt:0005785
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003312	mir-84	B0395.4	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035