WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  H1.X::GFP is expressed in body-wall muscles, as well as in the vulva sex muscles. In both cases the general appearance of the cells corresponds to the situation already described for the marginal cells of the pharynx: the fluorescence signal fills the cytoplasm, and the cells' nuclei appear as the brightest fluorescent structure. H1.X presence in muscle cells was not detected with the two different antibodies. H1.X::GFP was also expressed in a limited number of head neurons, in which the fluorescence signal filled the total cytoplasm, including the neuronal projections. The presence of H1.X in these cells was confirmed with the H1.X-101 antibody. Furthermore, H1.X::GFP was detected in the cytoplasm of excretory cells. In C. elegans embryos, H1.X::GFP expression starts with the 30-cell stage. The most prominent structures labeled with both antibodies as well as with H1.X::GFP in transgenic animals are the marginal cells and the tonofilaments therein. The signal appears in all larval stages and in adult C. elegans. No further nuclear or cytoplasmic signal appears besides the labeling of all nuclear lamina caused by the anti-lamin crossreactivity of H1.X-11. This result is confirmed by independent antibody labeling with H1.X-101. In these preparations no nuclear signal appeared in the nuclei of the marginal cells, although the cytoplasm of some head neurons was stained. Primary Identifier  Expr1926
Remark  hil-1 = C30G7.1 = H1.X Subcellular Localization  A double labeling experiment was performed with H1.X-11 and the monoclonal antibody IFA, which stains the intermediate filament proteins of the tonofilaments. Both antibodies label identical structures - the tonofilaments in the marginal cells. Antibody labeling with H1.X-101 revealed a prominent labeling of the nucleoli in the polyploid gut nuclei of adult C. elegans hermaphrodites, which colocalized with fibrillarin, a structural component of small nucleolar RNPs (snoRNPs). H1.X was never detected in condensed mitotic or meiotic chromosomes or as a structural component of the interphase chromatin, as revealed by antibody labeling ( H1.X-11) of embryos and meiotic oocytes. H1.X::GFP was never found localized to condensed chromosomes. H1.1-GFP fluorescence is readily observed in condensed mitotic chromosomes in C. elegans embryos, and it is detected as a structural component of interphase chromatin in antibody labeling of embryonic blastomeres and all other cell types of C. elegans. H1.X-101 did not label the nuclear lamina. In H1.X::gfp transgenic animals, the cytoplasm of the marginal cells appeared brightly fluorescent, and the nuclei therein became visible as the brightest fluorescent structures of the cells. The GFP fluorescence was almost homogeneously distributed throughout the whole cytoplasm, but the tonofilaments therein became clearly visible as bright green dots when visualized in axial orientation.
Paste the following link
Lists
This ExpressionPattern isn't in any lists. Upload a list.

6 Anatomy Terms

Definition Name Synonym Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
muscle associated with hermaphrodite vulva. vulval muscle   WBbt:0005821
neuron with its cell body situated in the head, excluding the pharynx. head neuron   WBbt:0006751
H-shaped cell associated with the excretory system, largest cell in C. elegans. excretory cell excretory canal cell WBbt:0005812
  excretory system   WBbt:0005736
a type of pharyngeal cell that connects basement membrane to the apices of triangle-shaped pharyngeal lumen. marginal cell   WBbt:0003673

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00001852 hil-1 C30G7.1 Caenorhabditis elegans

9 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over. elongating embryo Ce WBls:0000015
  The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing. enclosing embryo Ce WBls:0000013
  The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation. gastrulating embryo Ce WBls:0000010