WormMine: ExpressionPattern Expr13716

• Pattern: The Pttr-33::gfp reporter was found to be expressed in six cells residing in the head of L1 larvae. Two of the labelled cells were located next to the first pharyngeal bulb and four were situated halfway between the mouth and the first pharyngeal bulb. All six cells form protrusions towards the mouth of the larva. The cells were labelled as early as in the embryonic pretzel stage. Comparison with the cell atlas of C. elegans and a GFP reporter under the control of the grl-27 arcade cell-specific promoter revealed that these cells represent most likely the posterior arcade cells, which are located between external epidermis and anterior pharynx. In addition, in L1 stage larvae, ttr-33 expression was observed in the pharynx and in several unidentified cells immediately posterior to the second pharyngeal bulb. In older animals, the Pttr-33::GFP transcriptional reporter highlights head cells next to and anterior of the first pharyngeal bulb, as well as the pharynx and the hypodermis.
• Primary Identifier: Expr13716

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
anterior-most body region containing the pharynx.	head		WBbt:0005739
Interface between pharynx and hypodermis, form anterior part of the buccal cavity.	posterior arcade cell	arc post	WBbt:0005795

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00016505	ttr-33	C37C3.13	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020