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Expr4429
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Expressed in the hypodermis from embryo stage through adulthood. Expressed in the seam cells from embryo through adult. Expressed in the rectal epithelial cells from L1 and maintained through adulthood. Expressed in anterior and posterior arcades. Expressed in the excretory duct and pore cells. |
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Expr4461
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Expressed in anterior and Posterior arcades. |
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Expr4462
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Expressed in the seam cells. Expression decrease in old adults. Expressed in anterior and Posterior arcades. |
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Picture: Fig 3. |
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Expr8673
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Expression in the alimentary canal: Strong and consistent expression in anterior arcades, posterior arcades, M1, B cell. Weak or rare expression in intestine. Expression in the nervous system: Amsh, CEPsh, CEPso, ILso, OLso, Phso, DVC, MI. Expression in the reproductive system: In adult stage, expressed in gonad sheath, uterus, vulva(low), spermatheca, Sp-ut valve. In developing larva stage, expressed in uterus, vulva, spermatheca, Sp-ut valve. inx-5 first appears in the developing hypodermis at bean stage and then in the excretory cell at three-fold stage. |
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Expr13716
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The Pttr-33::gfp reporter was found to be expressed in six cells residing in the head of L1 larvae. Two of the labelled cells were located next to the first pharyngeal bulb and four were situated halfway between the mouth and the first pharyngeal bulb. All six cells form protrusions towards the mouth of the larva. The cells were labelled as early as in the embryonic pretzel stage. Comparison with the cell atlas of C. elegans and a GFP reporter under the control of the grl-27 arcade cell-specific promoter revealed that these cells represent most likely the posterior arcade cells, which are located between external epidermis and anterior pharynx. In addition, in L1 stage larvae, ttr-33 expression was observed in the pharynx and in several unidentified cells immediately posterior to the second pharyngeal bulb. In older animals, the Pttr-33::GFP transcriptional reporter highlights head cells next to and anterior of the first pharyngeal bulb, as well as the pharynx and the hypodermis. |
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Picture: Fig 3. |
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Expr8677
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Expression in the alimentary canal: Strong and consistent expression in anterior arcades, posterior arcades. Weak or rare expression in pm2, pm3, pm4, pm5, pm6, pm7, pm8, mc1, g1, g2, rectal gland cells, rectal epithelial cells. Expression in the nervous system: Phsh, AVK, DVC (early larva), PVR, SIB (early larva), URB, I3. Expression in the reproductive system: In adult stage, expressed in gonad sheath, uterus, vulval muscle. In developing larva stage, expressed in vulva. Neuronal expression of inx-9 appears around three-fold stage. The rectal gland expresses inx-9 during early larval stages. inx-9 is expressed in adult hermaphrodite sex muscles. inx-9 was expressed at high levels in arcade cells starting around two-fold stage continuing throughout development and adulthood. |
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Picture: Fig 3. |
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Expr8671
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Expression in the alimentary canal: Strong and consistent expression in pharyngeal epithelium, pm5, pm6, pm7, pm8, g2, rectal gland cells. Weak or rare expression in anterior arcades, posterior arcades, pm2, pm3, pm4, M3, MC, intestine, rectal epithelial cells. Expression in the nervous system: CEPsh, ALN, ASn, CAN, DAn, DBn, DDn, DVA, DVB, HSN, PDE, PLM, PVQ, PVR, PVT, URB, VAn, VBn, VDn, M3, MC. Expression in the reproductive system: In adult stage, expressed in spermatheca, vulval muscle, HSN. In developing larva stage, expressed in vulval muscle, uterine muscle, HSN. inx-3 was expressed broadly during early embryogenesis. After the beginning of morphogenesis, inx-3 expression becomes more restricted to the pharynx, hypodermis, and intestine. By three-fold stage inx-3 expression appears in ventral cord motor neurons (strongest in DA neurons) along with continued strong pharyngeal expression. By hatching, its hypodermal expression disappears, while postembryonically born ventral cord motor neurons express it at low levels. Its pharyngeal (strong) and neuronal (faint) expressions continue to adulthood. |
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Picture: Fig 3. |
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Expr8679
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Expression in the alimentary canal: Strong and consistent expression in anterior arcades, posterior arcades, pharyngeal epithelium, pm4, pm8, g1, g2, vir, K.a/K' cells. inx-11 is more strongly expressed in the most posterior (int 9) intestinal cell. Weak or rare expression in pm1, pm2, pm3, pm5, pm6, pm7. Expression in the nervous system: CEPsh, DVC, LUA. Expression in the reproductive system: In adult stage, expressed in utse. In developing larva stage, expressed in uterus, sperm (spermatocytes, spermatids). Expression of inx-11 appears in pharyngeal tissue around two-fold stage, and by three-fold stage, strong expression becomes restricted to g1, g2, pm4, and pm8. inx-11 is expressed in the hypodermal cells of the animal in postembryonic stages. |
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Picture: Fig 3. |
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Expr8694
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Expression in the alimentary canal: Strong and consistent expression in M5, I1, I3, I6, NSM. Weak or rare expression in posterior arcades. Expression in the nervous system: Phsh, ADA, ADE, ADL, AIN, AIY, ALM, AUA, AVA, AVD, AVH, AVJ, AVK, AVM, AWB, BDU, CAN, CEP, DAn, DBn, DDn, DVB, DVC, FLP, HSN, IL1, IL2, LUA, OLL, PDA, PDB, PDE, PHA, PHB, PHC, PLM, PLN, PVC, PVD, PVM, PVN, PVP, PVQ, PVR, PVT, PVW, RIB, RIC, RIF, RIP, RIS, RME, SDQ, SIA (early larva), SIB (early larva), SMB (early larva), SMD (early larva), URA, URB, VAn, VBn, VCn, VDn, M5, I1, I6, NSM. Expression in the reproductive system: In adult stage, expressed in vulval muscle, uterine muscle, HSN, VCn. In developing larva stage, expressed in HSN, VCn, and anchor cell. |
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Picture: N.A. |
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Expr8680
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Expression in the alimentary canal: Strong and consistent expression in M2, rectal epithelial cells. Weak or rare expression in anterior arcades, posterior arcades. Expression in the nervous system: Amsh, CEPso, ILso, OLso, PDEso, AVH (early larva), AVJ (early larva), AVK, CAN, IL1 (early larva), PDE, PVD, SIB (early larva), URB, VAn, VBn, M2. Expression in the reproductive system: In adult stage, expressed in vulva, spermatheca, sperm(spermatocytes, spermatids). In developing larva stage, expressed in vulva. inx-12 expressionstarts in seam precursors around 1.5-fold stage, followed by expression in arcade cells by 2-fold and head neurons by 3-fold stage. inx-12 is expressed in the hypodermal cells of the animal in postembryonic stages. |
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Picture: Fig 3A. |
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Expr9114
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Both methods revealed LET-23 expression in the ALA neuron, identified by the position of its cell body in the dorsal ganglion and its two lateral axonal projections, in two ventral head neurons that send projections into the nerve ring, and in a single tail neuron. LET-23 was also expressed in the pharyngeal-intestinal valve as well as in six cells anterior to the first pharyngeal bulb that project to a ring around the buccal cavity and are likely to be to the posterior arcade epithelial cells. |
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Reporter gene fusion type not specified. |
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Expr1614
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At the beginning of embryonic morphogenesis, all ten pairs of lateral epidermal cells, the seam cells, express cdh-3::lacZ. cdh-3::GFP expression in these cells is observed at hatching and throughout subsequent postembryonic development. During the last larval stage (L4) the 15 pairs of seam cells generated during larval development fuse to form two continuous lateral syncytia, surrounded by hyp7. cdh-3::GFP expression correlates with seam cell identity during these postembryonic divisions; it is not observed in daughters that fuse with hyp7 or adopt other fates. In embryos undergoing morphogenesis, lacZ is expressed in a single large nucleus, whose size and position is consistent with that of the excretory cell. This identification is reinforced by the observation that in many newly hatched L1 larvae, low levels of GFP expression are visible in the excretory cell. This expression of cdh-3-reporter genes was observed only during late embryogenesis and in some newly hatched L1s (the latter may be due to perdurance of the GFP fusion protein), but not at later stages. Several other cells expressed the cdh-3 reporter constructs during embryonic morphogenesis; as in the seam cells this expression continued upon hatching. In the tail two cells, hyp10 and hyp11, show strong GFP expression that persists only during the first larval stage. Strong expression was observed in cells that form interfacial epithelia between the intestinal epithelium and the epidermis. Two cells that form part of the rectal epithelium, designated F and U, express cdh-3::GFP during embryonic morphogenesis and throughout larval development. In the anterior of embryos undergoing morphogenesis there were several cells expressing cdh-3::lacZ, and in larvae and adults, GFP expression is seen in nine cell bodies located just anterior to the first bulb of the pharynx. Processes extend anteriorly from these cell bodies and terminate at the level of the buccal capsule. Based upon the location of the cell bodies and the morphology of the processes, these cells were identified as the anterior and posterior arcade cells. Expression pattern of the pJP#38 construct in males indicated expression in the male tail and several male-specific neurons, however the expression pattern is complicated and the cell identity were not determined. cdh-3::GFP expressed in the developing hermaphrodite vulva. GFP first expressed in the anchor cell in L3 larvae. A little later expression were seen in those vulval cells that are closest to the anchor cell and are beginning to invaginate. As vulval morphogenesis continues all of the cells that invaginate to form the vulva are expressing GFP. During this period, the uterine epithelium closest to the invaginating vulval cells begins to express cdh-3::GFP and the anchor cell fuses with the multinucleate uterine seam cell (utse), which also begins to express cdh-3::GFP. Expression continues in these cells into the adult stage, though at somewhat reduced levels, which may perhaps be due to perdurance of the fusion protein, since older adults show much reduced fluorescence compared with younger ones. During the time that the vulva is forming, cdh-3::GFP expressed in the six VC neurons located in the ventral nerve cord and the two HSNs located just posterior and dorsal to the developing vulva. These cells begin to extend processes at about this time, and GFP expression continues in these cells and their processes throughout the remainder of larval development and into adulthood. |
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