WormMine: ExpressionPattern Expr2590

• Pattern: Animals expressing the daf-12::GFP construct display the highest levels of GFP throughout the procorpal region in the pharyngeal muscle (pm3) cells. GFP is also expressed throughout the metacorpus (pm4 cells) as well as the isthmus (pm5 cells) and portions of the terminal bulb (pm6). These transgenic animals appear to express GFP throughout the muscles of the pharynx and the pattern of expression is similar to uorescently labeled phalloidin which stains muscles in C. elegans. GFP orescence is detected in each developmental stage including dauer larvae. L2d animals, grown on pheromone plates, express GFP in the same cells, though it appeared brighter than animals of the other stages.
• Primary Identifier: Expr2590
• Remark: Reporter gene fusion type not specified.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
third pharyngeal muscle cell layer	pm3	M3	WBbt:0003740
fifth pharyngeal muscle cell layer	pm5	M5	WBbt:0003737
fourth pharyngeal muscle cell layer	pm4	M4	WBbt:0003739
sixth pharyngeal muscle cell layer	pm6	m6	WBbt:0003724

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000908	daf-12	F11A1.3	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	A third stage larva specialized for dispersal and long term survival.		dauer larva Ce	WBls:0000032
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	A special second stage larva with altered physiology and developmental potential that leads to formation of dauer larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2d larva Ce	WBls:0000046