WormMine: ExpressionPattern Expr8169

• Pattern: At hatching, hlh-3 is expressed in most of the neurons of the nerve ring ganglia. This expression is undetectable by the early L1 larval stage. The most conspicuous expression during L1 is within the P cell lineages. Specifically, hlh-3 is expressed in the ectodermal-like P cells, and expression persists in the primary and secondary neural precursors of the P cell lineages. In addition, it appears to be expressed in all 53 of the resulting postmitotic motor neurons. Expression in most of these neurons is undetectable by the L2 larval stage. It is worth noting here that these neurons represent 77% of all motor neurons mediating locomotion. hlh-3 is also expressed in the HSNs and ventral type C (VC) motor neurons throughout larval development. At hatching, expression in the HSNs is weak, but peaks at the L3 larval stage and persists until the late L4 larval stage. Expression in the VCs also peaks during L3. Overall, hlh-3 appears to be expressed in all neural precursors. Based on expression in the P cell lineages, expression generally persists through terminal cell division and disappears from postmitotic neurons shortly thereafter. However, hlh-3 is also expressed in non-neural cells such as the P cells, as well as postmitotic, differentiating neurons such as the HSNs and VCs.
• Primary Identifier: Expr8169
• Remark: Embryonic expression of hlh-3 is widespread and complex, essentially precluding unambiguous identification of cells. Thus, authors chose to focus on postembryonic expression. Picture: Figure 1.
• Subcellular Localization: The translational GFP fusion is observed only in nuclei.

17 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Neuron class of six motoneurons in the ventral cord, which innervate ventral body muscles and the vm2 muscles of the vulva.	VC neuron		WBbt:0005304
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
neuron making synapse on an effector (muscle, gland).	motor neuron	motoneuron	WBbt:0005409
The tenth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P10		WBbt:0006779
The ninth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P9		WBbt:0006778
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1	P11	lineage name: ABplapappa	WBbt:0004410
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1	P12	lineage name: ABprapappa	WBbt:0004409
The sixth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P6		WBbt:0006775
The fifth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P5		WBbt:0006774
The fourth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P4		WBbt:0006773
The third (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P3		WBbt:0006772
The eighth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P8		WBbt:0006777
The seventh (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P7		WBbt:0006776
The second (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P2		WBbt:0006771
The first (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P1	P1 post-embryonic blast cell	WBbt:0006770

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001950	hlh-3	T24B8.6	Caenorhabditis elegans

0 Life Stages