WormMine: ExpressionPattern Expr10636

• Pattern: The aqp-8p::gfp-derived transcriptional GFP reporter exhibits uniform labeling of the excretory canal cytoplasm, whereas aqp-8p::aqp-8::gfp- derived translational AQP-8::GFP fusion proteins identify the subcellular canalicular compartment. AQP-8 is also expressed outside the excretory system. Low levels of AQP-8 can be detected in several other tissues, such as coelomocytes, a posterior intestinal cell pair (INT 9), rectum, vulval muscles, male germline and ray, hypodermic or mesodermal cell near the pharynx, pharynx muscle.
• Primary Identifier: Expr10636
• Reporter Gene: [aqp-8p::aqp-8::gfp] and [aqp-8p::aqp-8::mCherry] translational fusions. aqp-8 full-length genomic DNA with a 1.0 kilobase (kb) upstream sequence was amplified by PCR and cloned into the GFP vector pPD95.75 by Sph1 and BamH1. The amplified mCherry DNA was ligated to the 3' end of the aqp-8 full-length genomic DNA by BamH1-KpnI into pPD95.75. [aqp-8p::gfp] transcriptional fusion.

12 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
The passageway in the hindgut between the posterior intestine, the rectal valve and the opening to the exterior.	rectum		WBbt:0005773
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775
Head mesodermal cell, function unknown	head mesodermal cell	hmc	WBbt:0004697
male sensory organ; nine bilateral pairs of finger-like rays (numbered 1 to 9, Left/Right) radiate from the tail and are embedded in a cuticlar fan.	ray	male sensory ray	WBbt:0006941
cylindrical hypodermal syncytium in the head, including hyp1-hyp6	anterior hypodermis	head hypodermis	WBbt:0005373
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850
Intestinal cell of the nineth intestinal ring, right side.	int9R embryonic	lineage name: Eprppp	WBbt:0004342
Intestinal cell of the nineth intestinal ring, left side.	int9L embryonic	lineage name: Eplppp	WBbt:0004347

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000176	aqp-8	K02G10.7	Caenorhabditis elegans

0 Life Stages