WormMine: AnatomyTerm WBbt:0004342

• Definition: Intestinal cell of the nineth intestinal ring, right side.
• Name: int9R embryonic
• Primary Identifier: WBbt:0004342
• Synonym: lineage name: Eprppp

4 Children

Definition	Name	Synonym	Primary Identifier
Post-embryonic, binucleated int9R.	int9R post-embryonic		WBbt:0008238
nucleus of pedigree Eprppp	Eprppp nucleus	int9R nucleus	WBbt:0002174
post-embryonic cell of pedigree E.prpppa	E.prpppa		WBbt:0007133
post-embryonic cell of pedigree E.prpppp	E.prpppp		WBbt:0007134

0 Expression Clusters

10 Expression Patterns

• Primary Identifier: Expr3749
• Pattern: In transgenic animals that express VAV-1::GFP, strong GFP fluorescence was observed in the pharynx, proximal gonad, spermatheca, intestine, and rectal epithelia. In the pharynx, VAV-1::GFP was observed in most cell types, including muscle cells, marginal cells, epithelial cells, neurons, and gland cells. In the gonad, VAV-1::GFP was observed in the contractile sheath cells adjacent to the spermatheca. The expression of VAV-1::GFP in the intestine was limited to the four most posterior cells (int8 and int9) and the three rectal epithelial cells. Additionally, VAV-1::GFP expression was observed in the distal gonad, body-wall muscle, and vulval epithelia.

• Primary Identifier: Expr163
• Remark: Other strain-- UL844
• Pattern: Expression is seen in the cells of the E-lineage from the 8E cell stage. Consistent with this expression is seen in all intestinal cells in larvae and adults. Some anterior expression in the head hypodermis and pharynx is also occasionally seen.

• Primary Identifier: Expr103
• Remark: Other strain-- UL123
• Pattern: This strain exhibits strong expression in the embryo. Expression is first seen in the 50-80 cell embryo and extends through to adulthood. It appears that most of the AB cells in the embryo stain, and what appears to be the cells of the C lineage. Some embryos exhibit staining in the two rows of nuclei that are the E lineage. All embryonic staining is very intense, and it spreads to the cytoplasm giving blue embryos, therefore obscuring the DAPI staining, making it difficult to count the number of cells in the embryos as each component begins expressing. This intense staining fades as the embryo ages, sometimes leaving blue comma stage embryos with no distinct nuclei staining. Hypodermal expression is seen in the 3 fold stage of embryogenesis and in young larvae which most probably are C-derived hyp-7 nuclei. Expression weakens as the worm gets older and is much less frequently expressed in adults. Some adults do show staining in the anterior hypodermal nuclei (hyp-3, hyp-4) and in the anterior hypodermal seam cells, also some nuclei stain in the tail.

• Primary Identifier: Expr118
• Remark: Other strain-- UL511
• Pattern: Expression is first seen in precomma stage embryos, in as yet unidentified nuclei. In comma stage embryos staining is seen in the intestinal precursors, and other cells including possibly members of the C cell lineage. In 3-fold embryos and through till L3 stage larvae, expression appears as pairs of nuclei down the body starting anteriorly of the metacorpus, probably hyp-7. There is also a lot of diffuse staining in the head and pharynx. In larvae strong expression is seen in the intestinal nuclei. At L4 stage this expression starts to fade, and fewer nuclei are seen to stain. In adults the expression is reduced to a handful of nuclei (from 1-10) around the pharynx. Sometimes in adults a few anterior intestinal nuclei continue to show expression.

• Primary Identifier: Expr10636
• Reporter Gene: [aqp-8p::aqp-8::gfp] and [aqp-8p::aqp-8::mCherry] translational fusions. aqp-8 full-length genomic DNA with a 1.0 kilobase (kb) upstream sequence was amplified by PCR and cloned into the GFP vector pPD95.75 by Sph1 and BamH1. The amplified mCherry DNA was ligated to the 3' end of the aqp-8 full-length genomic DNA by BamH1-KpnI into pPD95.75. [aqp-8p::gfp] transcriptional fusion.
• Pattern: The aqp-8p::gfp-derived transcriptional GFP reporter exhibits uniform labeling of the excretory canal cytoplasm, whereas aqp-8p::aqp-8::gfp- derived translational AQP-8::GFP fusion proteins identify the subcellular canalicular compartment. AQP-8 is also expressed outside the excretory system. Low levels of AQP-8 can be detected in several other tissues, such as coelomocytes, a posterior intestinal cell pair (INT 9), rectum, vulval muscles, male germline and ray, hypodermic or mesodermal cell near the pharynx, pharynx muscle.

• Primary Identifier: Expr1585
• Remark: F-11 antigen cDNA was isolated and found located on C05E4, with GenBank accession number U23159. Blast search traced it to C05E4.14, also known as srh-2.
• Pattern: Expression associated with developing intestine and body wall muscle cells. About 4-6 faintly labeled E-derived cells were detected at about 100-cell embryos, Eight E-derived cells were more prominently labeled in 250-cell embryo. Body wall muscle cells were first detected with the mAb in addition to the complement of 20 intestinal cells at about 450-cell embryos. Native gut fluorescent was not visible.

• Primary Identifier: Expr2123
• Remark: Clone = pUL#L450A Strain = UL925
• Pattern: Expression is seen in the E-cell lineage and intestinal cells throughout embryonic development and in early larval stages. This gene has homology to hexose transporters.

• Primary Identifier: Expr672
• Remark: This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).
• Pattern: First detection at 2E cell stage and persists in E lineage throughout embryogenesis (expression pattern same as Ab).

• Primary Identifier: Expr673
• Remark: This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).
• Pattern: ELT-2 protein first detected at 2E cell stage (half way through cell cycle and following E-cell ingression associated with gastrulation). At 44-46 cell stage, expression is observed in 2E cells. During all subsequent stages of embryogenesis, ELT-2 protein is detected in all gut lineage, staining is nuclear localized. Protein persists in nuclei of all gut cells, in all larval stages, as well as in adults. Above pattern for male and hermaphrodite.

• Primary Identifier: Expr10593
• Pattern: Consistent GFP expression was observed in the posteriormost int9 intestinal cells. Weaker expression was observed with lower penetrance in the four cells of the anteriormost int1 intestinal cells. No expression was observed in the body-wall muscle, enteric muscle, or neurons.

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016

3 Parents

Definition	Name	Synonym	Primary Identifier
embryonic cell	Eprpp		WBbt:0006277
any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.	intestinal cell		WBbt:0005792
cell that exists in and is part of an embryo.	embryonic cell		WBbt:0007028