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WS294

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Expression Pattern :

Pattern  The pry-1 reporter gene is widely expressed throughout development. Expression starts halfway through embryogenesis and is mainly localized to the ventral and lateral hypodermal cells. At the early L1 stage, pry-1 is expressed at high levels in the lateral hypodermal cells (or seam cells) V5 and V6 and in the Q neuroblasts QL and QR. pry-1 is also expressed in the ventral hypodermal (P) cells P7/8 to P11/12, body wall muscle cells, and neurons in the head, the tail, and the ventral nerve cord. No differences in pry-1 expression levels between QL and QR was observed, but this may be a result of PRY-1 GFP overexpression. At the end of the L1 stage, pry-1 is expressed at high levels in all seam cells. Expression was also observed in the QL and QR daughter cells. At later larval stages, pry-1 is expressed at high levels throughout the animal, including hypodermal cells, body wall muscle cells, and many neurons in the ventral nerve cord and head and tail ganglia. In addition, pry-1 is expressed in the vulva precursor (Pn.p) cells and in the developing vulva and male tail. Primary Identifier  Expr1896
Remark  The expression pattern of this construct was similar in independent transgenic lines, but the subcellular localization of the PRY-1 GFP fusion protein differed, ranging from localization at the plasma membrane and in cytoplasmic dots to diffuse cytoplasmic and nuclear staining. This difference in subcellular localization may be a consequence of variations in expression levels of the fusion protein in different transgenic lines. For ease of cell identification, a transgenic line showing diffuse cytoplasmic and nuclear staining was selected. This transgene fully rescued the lethality, the multivulva phenotype, and the QR.d migration defect of pry-1(mu38 and nc1). This suggests that the PRY-1 GFP fusion protein is functional and is correctly expressed in cells in which PRY-1 is essential.
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58 Anatomy Terms

Definition Name Synonym Primary Identifier
neuron with cell body associated with the ventral nerve cord. ventral cord neuron ventral cord motoneuron WBbt:0005300
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
neuron with its cell body situated in the head, excluding the pharynx. head neuron   WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum. tail neuron   WBbt:0006759
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite. P5.p hermaphrodite   WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite. P4.p hermaphrodite   WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite. P3.p hermaphrodite   WBbt:0008112
  P8.p hermaphrodite   WBbt:0008133
  P7.p hermaphrodite   WBbt:0008129
  P6.p hermaphrodite   WBbt:0008125
The tenth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P10   WBbt:0006779
The ninth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P9   WBbt:0006778
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1 P11 lineage name: ABplapappa WBbt:0004410
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1 P12 lineage name: ABprapappa WBbt:0004409
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar. vulF   WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr. vulE   WBbt:0006767
Posterior daughter cell of P8.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P8.pp lineage name: P8.pp WBbt:0004145
Posterior daughter cell of P3.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P3.pp lineage name: P3.pp WBbt:0004153
Anterior daughter cell of P4.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P4.pa lineage name: P4.pa WBbt:0004151
Posterior daughter cell of P4.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P4.pp lineage name: P4.pp WBbt:0004149
Anterior daughter cell of P8.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P8.pa lineage name: P8.pa WBbt:0004147
Posterior daughter cell of P5.pp, fuses into hermaphrodite vulD syncytium. P5.ppp lineage name: P5.ppp WBbt:0004477
Anterior daughter cell of P7.pa, fuses into hermaphrodite vulD syncytium. P7.paa lineage name: P7.paa WBbt:0004448
Anterior daughter cell of P3.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P3.pa lineage name: P3.pa WBbt:0004155
cell, anterior daughter of P6.p P6.pa   WBbt:0006987
cell, posterior descendent of P7.p P7.pp   WBbt:0006984
cell, anterior descendent of P7.p P7.pa   WBbt:0006983
cell, anterior daughter of P6.pp P6.ppa   WBbt:0006991
cell, posterior daughter of P6.pa P6.pap   WBbt:0006990
cell, anterior daughter of P6.pa P6.paa   WBbt:0006989

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00004202 pry-1 C37A5.9 Caenorhabditis elegans

0 Life Stages