WormMine: ExpressionPattern Expr15685

• Pattern: As expected, we observed extensive GFP::TRA-1 fluorescence in multiple tissues of larval and adult hermaphrodites. Surprisingly, however, we also detected significant GFP::TRA-1 in males, confined almost exclusively to the nervous system. In L3 larval males, GFP::TRA-1 was apparent in numerous head neurons, though not in as many as seen in L3 hermaphrodites. This pattern was maintained in L4 males, but, by the time of young adulthood, far fewer neurons contained GFP::TRA-1.Using a pan-neural marker, we quantified the number of GFP::TRA-1-positive neurons in the region spanned by the pharyngeal bulbs. This revealed that tra-1 is expressed in roughly 54 and 49 head neurons in L3 and L4 males, respec- tively, and that this number decreases to 25 in 1-day-old adult males. Using a marker for ciliated sensory neurons, we found that roughly 17 of the tra-1-expressing neurons in this region in L3 males were of this class, and that this number decreased to 9 in 1-day-old adult males. These findings suggest that tra-1 function in the nervous system might not be limited to hermaphrodites, and that any roles it may have in the male soma could be both cell type and stage specific.
• Primary Identifier: Expr15685

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006604	tra-1	Y47D3A.6	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035