WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00006604 Gene Name  tra-1
Sequence Name  ? Y47D3A.6 Brief Description  tra-1 encodes Zn2+-finger-containing proteins that are the sole C. elegans members of the GLI transcription factor family; during development, tra-1 functions cell autonomously as the terminal regulator of the sex determination pathway and positively regulates all aspects of hermaphrodite somatic sexual differentiation; in addition, tra-1 activity is required for proper development of the male somatic gonad and for sustained spermatogenesis in both sexes; in regulating sex-specific development, TRA-1 functions, in part, by repressing transcription of mab-3 and egl-1 in the intestine and HSN neurons, respectively; TRA-1 is expressed in hermaphrodites and males and localizes to both the nucleus and cytoplasm of many different cell types; expression of C-terminally truncated TRA-1 isoforms is much higher in hermaphrodites than males, due to sex-specific proteolysis that results in the presence of feminizing TRA-1 isoforms in hermaphrodites.
Organism  Caenorhabditis elegans Automated Description  Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity and RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding activity. Involved in developmental process involved in reproduction; negative regulation of transcription by RNA polymerase II; and positive regulation of neuron apoptotic process. Located in cytoplasm and nucleus. Expressed in several structures, including AWA; germ line; gonad; intestine; and somatic cell. Human ortholog(s) of this gene implicated in several diseases, including Culler-Jones syndrome; gastrointestinal system cancer (multiple); and synostosis (multiple). Is an ortholog of human GLI1 (GLI family zinc finger 1); GLI2 (GLI family zinc finger 2); and GLI3 (GLI family zinc finger 3).
Biotype  SO:0001217 Genetic Position  III :6.88967 ±0.104832
Length (nt)  ? 24312
Quick Links:
 

1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00006604

Genomics

2 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:Y47D3A.6a.1 Y47D3A.6a.1 4673   III: 11170890-11195201
Transcript:Y47D3A.6b.1 Y47D3A.6b.1 1289   III: 11182016-11195201
 

Other

2 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:Y47D3A.6a Y47D3A.6a 3333   III: 11172092-11172244
CDS:Y47D3A.6b Y47D3A.6b 867   III: 11182300-11182497

22 RNAi Result

WormBase ID
WBRNAi00095789
WBRNAi00009343
WBRNAi00056762
WBRNAi00026721
WBRNAi00077788
WBRNAi00006884
WBRNAi00007178
WBRNAi00037165
WBRNAi00071366
WBRNAi00107577
WBRNAi00056763
WBRNAi00075580
WBRNAi00065768
WBRNAi00070198
WBRNAi00056764
WBRNAi00056765
WBRNAi00107159
WBRNAi00107108
WBRNAi00107453
WBRNAi00107435
WBRNAi00107605
WBRNAi00107502

603 Allele

Public Name
gk963887
otn11162
otn11163
q183
q245
q312
q138
rh132
otn13141
q88
WBVar02069745
WBVar00247294
WBVar00247295
WBVar00247296
WBVar00247297
WBVar00247298
WBVar01607282
WBVar01607283
WBVar01710173
WBVar01542332
WBVar01269312
WBVar01269319
WBVar01269318
WBVar01269315
WBVar01269313
WBVar01269321
WBVar01269320
WBVar01269329
WBVar01269325
WBVar01269324

1 Chromosome

WormBase ID Organism Length (nt)
III Caenorhabditis elegans 13783801  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00006604 11170890 11195201 -1

4 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  
Panther orthologue and paralogue predictions  

0 Downstream Intergenic Region

132 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
adult vs dauer larva Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C. N.A. WBPaper00050488:adult_vs_dauer_regulated_N2_20C
  mRNAs that showed decreased expression in 1 cell mebryo comparing to in oocyte, according to RNAseq analysis. Gaussian error propagation. As cutoff for the up-regulated genes authors used log2 fold change > 1 and P < 0.05 and as cutoff for the down-regulated genes authors used log2 fold change < -1 and P < 0.05. WBPaper00045420:fertilization_downregulated_transcript
Osmotic stress Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when food was present DESeq(version 1.10.1), FDR < 0.05. WBPaper00050726:OsmoticStress_regulated_Food
Osmotic stress Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when no food was present DESeq(version 1.10.1), FDR < 0.05. WBPaper00050726:OsmoticStress_regulated_NoFood
  Transcripts that showed significantly higher expression in somatic gonad precursor cells (SGP) vs. head mesodermal cells (hmc). DESeq2, fold change >= 2, FDR <= 0.01. WBPaper00056826:SGP_biased
  Transcripts expressed in the epithelial tissues surrounding the pharynx that includes the arcade and intestinal valve (AIV) cells, according to PAT-Seq analysis using Pbath-15-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:arcade_intestinal-valve_expressed
  Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:GABAergic-neuron_expressed
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:intestine_expressed
  Transcripts expressed in NMDA neuron, according to PAT-Seq analysis using Pnmr-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:NMDA-neuron_expressed
  Genes with expression level regulated by genotype (N2 vs CB4856) and age at old adults stage (214 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_age_regulated_aging
  Genes with expression level regulated by genotype (N2 vs CB4856) at old adults stage (214 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_regulated_aging
  Genes with expression level regulated by genotype (N2 vs CB4856) at Late reproduction stage (96 hours at 24 centigrade). Authors permuted transcript values and used a genome-wide threshold of log10 P-value = 2, which resembles a false discovery rate (FDR) of 0.0118. WBPaper00040858:eQTL_regulated_reproductive
Bacteria infection: Staphylococcus aureus MW2. 4 hours of exposure. Transcripts that showed significantly increased expression after N2 animals had 4 hours of infection by Staphylococcus aureus (MW2). DEseq 1.18.0, adjusted p-value < 0.05. WBPaper00056471:S.aureus-4h_upregulated_N2
  Transcripts that showed differential expression between 24 and 26 hours post hatching L2d and dauer committed larvae of daf-9(dh6), triggered by the dafachronic acid (DA) growth hormone. Cluster 3 genes increased expression transiently at 26 hour post hatching. Benjamini Hochberg corrected q-value < 0.01. WBPaper00053388:dauer_regulated_Cluster3
  Transcripts that showed significantly increased expression in oocyte germline cells comparing to in mitosis germline cells. Log2 Fold change > 2 or <-1, p-value < 0.05. WBPaper00053599:oocyte_vs_mitosis_upregulated
  Transcripts detected in body muscle nuclei according to a nuclear FACS-based strategy. Cufflinks WBPaper00065120:body-muscle-transcriptome
Reduced humidity (98% relative humidity). Genes that were down-regulated after one day exposure to reduced humidity (98% relative humidity) according to microarray analysis. Multiple hypothesis testing with the Benjamini-Hochberg correction was applied on calculated p-values. A change in the expression level was considered to be significant if the adjusted p-value was less than 0.001. WBPaper00044578:reduced-humidity_downregulated_microarray
  Genes that showed expression levels higher than the corresponding reference sample (L3/L4 all cell reference). A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes. WBPaper00037950:PVD-OLL-neurons_L3-L4-larva_expressed
  Transcripts that showed significantly decreased expression in eat-2(ad1116) comparing to in N2 at 3-days post L4 adult hermaphrodite animals. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:eat-2(ad1116)_downregulated
  Transcripts that showed significantly decreased expression after animals were treated with 100uM Psora and 250uM Allantoin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Psora-Allantoin_downregulated
  Transcripts that showed significantly decreased expression after animals were treated with 100uM Rapamycin and 250uM Allantoin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rapamycin-Allantoin_downregulated
  Transcripts that showed significantly decreased expression after animals were treated with 100uM Rapamycin and 50mM Metformin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rapamycin-Metformin_downregulated
  Transcripts that showed significantly altered expression in rnp-6(dh1127) animals comparing to in N2 when fed with heat killed E. coli OP50. Differentially expressed genes (DEGs) (q-value <0.05) between different samples were identified using the stringtie version 1.3.0, followed by Cufflinks version 2.2. WBPaper00059824:rnp-6(dh1127)_regulated_OP50
  Transcripts that showed significantly altered expression in rnp-6(dh1127) animals comparing to in N2 when fed with live S. aureus. Differentially expressed genes (DEGs) (q-value <0.05) between different samples were identified using the stringtie version 1.3.0, followed by Cufflinks version 2.2. WBPaper00059824:rnp-6(dh1127)_regulated_S.aureus
  Transcripts that showed significantly decreased expression after animals were treated with 100uM Rapamycin and 50uM Rifampicin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rapamycin-Rifampicin_downregulated
  Transcripts that showed significantly decreased expression after animals were treated with 50uM Rifampicin and 250uM Allantoin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rifampicin-Allantoin_downregulated
  Transcripts that showed significantly decreased expression after animals were treated with 50uM Rifampicin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rifampicin_downregulated
  Genes that were not enriched in either spermatogenic fem-3(q96gf) nor oogenic fog-2(q71) gonads, according to RNAseq analysis. To identify differentially expressed transcripts, authors used R/Bioconductor package DESeq. WBPaper00045521:Gender_Neutral

22 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr4780   In the intestine, TRA-1 is predominantly nuclear localized in both males and hermaphrodites. However, overall TRA-1 protein levels are significantly lower in wild-type male intestine cells. nuclear localized
No detailed description on cellular expression patterns.   Expr4331 In whole-mount indirect immunofluorescence experiments, the affinity-purified antibody detected nuclear-localized antigen in many tissues of adult tra-1(+) hermaphrodites. Staining was specific for TRA-1 proteins, because it was not detectable in pseudomales homozygous for the null allele tra-1(e1834). Wild-type males exhibited far weaker TRA-1 immunofluorescence than their hermaphrodite siblings; however, when TRA-1 was detectable in males, it was primarily nuclear. Therefore, TRA-1 proteins appear predominantly nuclear in both sexes and are present at much higher levels in hermaphrodites than in males. Expressed in nuclei.
    Expr2035614 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr13725 TRA-1 is expressed broadly throughout the entire nervous system. Unexpectedly, TRA-1 expression was found to be essentially undetectable in postmitotic neurons in early larval stages (L1, L2), suggesting that at these early larval stages the nervous system may not yet have acquired sexually dimorphic features. We found TRA-1 expression in postmitotic neurons of the nervous system to commence at the L3 stage, peak at the L4 stage, and persist well into adulthood.  
Also expressed in (comments from author) : Embryo incomplete. To be updated. Strain: BC12051 [tra-1::gfp] transcriptional fusion. PCR products were amplified using primer A: 5' [TCGGCAAACCAGCAATTT] 3' and primer B 5' [CCGTTTTCGTTGTTTTTGATTT] 3'. Expr6989 Adult Expression: intestine; Larval Expression: intestine;  
Also expressed in (comments from author) : Embryo incomplete. Strain: BC11897 [tra-1::gfp] transcriptional fusion. PCR products were amplified using primer A: 5' [TCGGCAAACCAGCAATTT] 3' and primer B 5' [CCGTTTTCGTTGTTTTTGATTT] 3'. Expr6988 Adult Expression: intestine; Nervous System; head neurons; Larval Expression: intestine; Nervous System; head neurons;  
    Expr15687 We detected no expression of GFP::TRA-1 in AWA in L3 males, although it was clearly apparent in AWA in L3 hermaphrodites  
    Expr1032735 Tiling arrays expression graphs  
    Expr3085 After hatching, TRA-1 was still detected in SGPs, but its level and subcellular distribution were distinct in the two sexes. In hermaphrodite L1 SGPs, TRA-1 was largely nuclear, but in male L1 SGPs, TRA-1 was present at a lower level and was uniformly distributed between nucleus and cytoplasm. A similar sexually dimorphic difference was observed in the distribution of GFP::TRA-1 in L1 SGPs. The GFP::TRA-1 reporter partially rescued tra-1(0) mutants, indicating that it produces a functional protein. After the first SGP division, TRA-1 continues to be expressed in hermaphrodite gonads, but is no longer detectable in males. In wild-type XX embryos, TRA-1 was predominantly nuclear in SGPs from formation of the primordium, through embryogenesis, and into the first larval stage. Next, embryos from a fog-2 male/female strain was examined , which produces 50% XX and 50% XO progeny, to determine if TRA-1 is similarly expressed in male SGPs. In 2-fold embryos, most SGPs possessed nuclear TRA-1 (27/30). Therefore, TRA-1 is present in SGP nuclei of both XX hermaphrodite and XO male embryos. Expressed in nuclear and cytoplasm.
Expression pattern in other life stages are not mentioned. --wjc.   Expr1746 In the germline of adult males, TRA-1 localization has some similarities and some important differences with that of hermaphrodites. Like hermaphrodites, TRA-1 is both nuclear and cytoplasmic throughout the germline. However, high levels of nuclear TRA-1 are found only in the mitotic zone. Costaining of male germlines with GLP-1 antibodies and To-Pro-3 indicated that the increased nuclear TRA-1 is coincident with GLP-1 expression and mitotic cells. NIH image analysis indicated that the high levels of staining in the mitotic zone are similar to those in hermaphrodites. However, in the transition zone and meiotic pachytene region, it is about 38% and 46% lower, respectively, than in hermaphrodites (n=6 germlines per sexual phenotype and about 15 nuclei per germline). In the hermaphrodite germline, TRA-1 is both nuclear and cytoplasmic. However, there are important regional differences, with the most distal mitotic region and the transition zone having the highest levels of nuclear TRA-1 and the meiotic pachytene region having the lowest. Costaining the germlines with GLP-1 antibodies and the DNA dye To-Pro-3 confirms the spatial distribution of the TRA-1 patterns. Quantitation of about ten germlines indicates high levels of nuclear TRA-1 are detected in the first 3040 nuclei along the germline axis. TRA-1 staining in the meiotic pachytene region is diffuse and distinct nuclei are not apparent. Using NIH image analysis, the levels of staining between the different zones were measured. Both the mitotic and transition zones had similar levels of TRA-1 staining while the meiotic pachytene region was reduced by approximately 40% (n=5 germlines and 15 nuclei were analyzed per zone). In the most proximal region of the meiotic zone, there is a drop in the overall levels of TRA-1. TRA-1 staining in L4 intestine, like the adult male, is diffuse. tra-2(lf) and tra-2(lf) fem-1(lf) tissues were examined to see whether the distribution of TRA-1 correlates with phenotypic sex. In strong tra-2(lf) XX mutants, TRA-1 nuclear levels are similar to wild-type XO males. In contrast, in tra-2(lf); fem-1(lf) XX double mutants, TRA-1 nuclear levels are similar to wild-type XX hermaphrodites. TRA-1 also has a sex-specific spatial distribution in the germline. The distribution of TRA-1 in the germlines of wild-type XX L4 hermaphrodites, tra-2(lf) and tra-2(lf); fem-1(lf) animals was also analyzed. Similar to the intestine, the distribution of TRA-1 is dependent on the sexual state of the tissue. In L4 hermaphrodite and tra-2(lf) germlines, which make only sperm, TRA-1 staining is similar to adult males; TRA-1 nuclear levels are high only in the mitotic region. However, in tra-2(lf); fem-1(lf) germlines, which make only oocytes, TRA-1 nuclear levels are high across the germline. The fact that TRA-1 nuclear levels are high throughout the germline suggests that fem-1 may regulate nuclear TRA-1 levels in the meitotic pachytene region of wild-type adult hermaphrodites. The distribution of TRA-1 in the intestine and gonad was analyzed by immunohistochemistry. In hermaphrodite and male intestines, TRA-1 is both nuclear and cytoplasmic; however, nuclear TRA-1 levels appear higher in hermaphrodites than in males. In males, the staining is diffuse, indicating an even distribution of TRA-1 between the nucleus and cytoplasm. Myc-TRA-1A is nuclear and cytoplasmic, with hermaphrodites having higher nuclear TRA-1 than males. TRA-1 is both nuclear and cytoplasmic in hermaphrodite and male intestines and germlines. However, in the germline, the levels of nuclear TRA-1 differ depending on the location of the germ cell within the gonad.
    Expr14645 TRA-2B:HA was detected exclusively in the nuclei of dissected tissues of larvae and adults, including those of the intestine and somatic gonad (sheath and distal tip cells). Nuclear localization of TRA-2B in somatic cells is consistent with previous studies using antibodies (Shimada et al., 2006) and reporter transgenes (Lum et al., 2000; Mapes et al., 2010)  
    Expr15685 As expected, we observed extensive GFP::TRA-1 fluorescence in multiple tissues of larval and adult hermaphrodites. Surprisingly, however, we also detected significant GFP::TRA-1 in males, confined almost exclusively to the nervous system. In L3 larval males, GFP::TRA-1 was apparent in numerous head neurons, though not in as many as seen in L3 hermaphrodites. This pattern was maintained in L4 males, but, by the time of young adulthood, far fewer neurons contained GFP::TRA-1.Using a pan-neural marker, we quantified the number of GFP::TRA-1-positive neurons in the region spanned by the pharyngeal bulbs. This revealed that tra-1 is expressed in roughly 54 and 49 head neurons in L3 and L4 males, respec- tively, and that this number decreases to 25 in 1-day-old adult males. Using a marker for ciliated sensory neurons, we found that roughly 17 of the tra-1-expressing neurons in this region in L3 males were of this class, and that this number decreased to 9 in 1-day-old adult males. These findings suggest that tra-1 function in the nervous system might not be limited to hermaphrodites, and that any roles it may have in the male soma could be both cell type and stage specific.  
Reporter gene fusion type not specified.   Expr2973 A tra-1::gfp transgene is expressed in Z1 and Z4 and their descendents, both in wild-type XX and XO L1 gonads (L.M. and J.K., unpublished).  
    Expr1020433 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  
Original chronogram file: chronogram.1698.xml [Y47D3A.6:gfp] transcriptional fusion. Chronogram669    
Original chronogram file: chronogram.1630.xml [Y47D3A.6:gfp] transcriptional fusion. Chronogram601    
    Expr14644 TRA-2:HA is detected in larval and adult hermaphrodites, but not in males, consistent with their greatly reduced transcript level.  
    Expr2017475 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr1505 There are two tra-1 transcription. One RNA is 5 kb, the other is 1.5 kb. The 5 kb RNA is most abundant in embryos, and then present at lower levels, throughout the rest of development. The 1.5 kb RNA is most abundant abundant during L2 in XX animals.  
No detailed cellular pattern description.   Expr1053   GFP-TRA-1A localized to nuclei in both XX and XO animals.
    Expr15686 We detected no expression of GFP::TRA-1 in AWA in L3 males, although it was clearly apparent in AWA in L3 hermaphrodites  
    Expr1160220 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  

39 GO Annotation

Annotation Extension Qualifier
  enables
has_input(WB:WBGene00000451) enables
  enables
  enables
  enables
has_input(WB:WBGene00001483) enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
has_input(WB:WBGene00001483) involved_in
  involved_in
  enables
  involved_in
  involved_in
  involved_in
  involved_in
has_input(WB:WBGene00007776),occurs_in(WBbt:0007849) involved_in
occurs_in(WBbt:0005175) involved_in
occurs_in(WBbt:0005175) involved_in

7 Homologues

Type
least diverged orthologue
least diverged orthologue
least diverged orthologue
least diverged orthologue
orthologue
least diverged orthologue
orthologue

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00006604 11170890 11195201 -1

39 Ontology Annotations

Annotation Extension Qualifier
  enables
has_input(WB:WBGene00000451) enables
  enables
  enables
  enables
has_input(WB:WBGene00001483) enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
has_input(WB:WBGene00001483) involved_in
  involved_in
  enables
  involved_in
  involved_in
  involved_in
  involved_in
has_input(WB:WBGene00007776),occurs_in(WBbt:0007849) involved_in
occurs_in(WBbt:0005175) involved_in
occurs_in(WBbt:0005175) involved_in

0 Regulates Expr Cluster

1 Sequence

Length
24312

1 Sequence Ontology Term