WormMine: ExpressionPattern Expr10551

• Pattern: DAF-18::GFP expression was observed in many tissues during all larval stages, including the developing vulva, the uterus, the ventral nerve cord and the distal tip cells. In particular, equal levels of DAF-18::GFP expression were detected in the six VPCs of L2 larvae, and expression persisted in the descendants of the induced VPCs until the Pn.pxxx stage. Besides the vulval cells, the DAF-18::GFP reporter was also expressed at the L3 to L4 larval stages in several cells of the uterus, which is part of the somatic gonad.
• Primary Identifier: Expr10551
• Subcellular Localization: The sub-cellular localization of DAF-18::GFP changed over the course of vulval development. In the VPCs of L2 larvae prior to and during induction (Pn.p stage), DAF-18::GFP was predominantly localized in the cytoplasm and the nucleus. However, at the subsequent stages (Pn.px to Pn.pxx stages), DAF- 18::GFP became increasingly localized to the plasma membrane of the vulval cells. Plasma membrane staining peaked at the 'Christmas tree' (Pn.pxxx) stage, when almost all the protein appeared to be localized to the membranes and nuclear staining was reduced to very low levels.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
somatic cell that situates at the tip of a gonad arm.	distal tip cell	DTC	WBbt:0006865
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000913	daf-18	T07A9.6	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035