WormMine: Gene WBGene00006540

• WormBase Gene ID: WBGene00006540
• Gene Name: tbg-1
• Sequence Name: F58A4.8
• Brief Description: tbg-1 encodes gamma-tubulin; in C. elegans, tbg-1 activity is essential and is required fornormal bipolar spindle assembly and function, as well as the fast phase of female pronuclearmigration following fertilization; while tbg-1 is required for proper organization and function of kinetochore and interpolar microtubules, it does not appear to be absolutely required for microtubule nucleation and growth; TBG-1 localizes to centrosomes in both interphase and mitotic cells, exhibiting a dynamic behavior during mitosis that differs between anterior and posterior centrosomes.
• Organism: Caenorhabditis elegans
• Automated Description: Predicted to enable GTP binding activity. Predicted to be a structural constituent of cytoskeleton. Involved in several processes, including maintenance of centrosome location; microtubule cytoskeleton organization; and regulation of cell cycle. Located in centrosome and cytoplasm. Expressed in gonad. Human ortholog(s) of this gene implicated in complex cortical dysplasia with other brain malformations 4 and inclusion body myositis. Is an ortholog of human TUBG2 (tubulin gamma 2).
• Biotype: SO:0001217
• Genetic Position: III :0.807824 ±0.007437
• Length (nt): 2619

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00006540

Genomics

1 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:F58A4.8.1	F58A4.8.1	1476	III: 9626536-9629154

Other

1 CDSs

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F58A4.8	F58A4.8	1335	III: 9626677-9626991

43 RNAi Result

• WBRNAi00085323
• WBRNAi00048935
• WBRNAi00077183
• WBRNAi00033065
• WBRNAi00099933
• WBRNAi00082397
• WBRNAi00077894
• WBRNAi00008894
• WBRNAi00071890
• WBRNAi00078513
• WBRNAi00080563
• WBRNAi00094837
• WBRNAi00082622
• WBRNAi00100808
• WBRNAi00071350
• WBRNAi00101295
• WBRNAi00099327
• WBRNAi00101238
• WBRNAi00101257
• WBRNAi00099731
• WBRNAi00101276
• WBRNAi00099529
• WBRNAi00101121
• WBRNAi00069756
• WBRNAi00076502
• WBRNAi00002572
• WBRNAi00022772
• WBRNAi00007702
• WBRNAi00114591
• WBRNAi00038544

29 Allele

• gk964518
• gk963887
• t1465
• gk944457
• gk887201
• gk754922
• gk559178
• gk462798
• gk927598
• gk562373
• gk903596
• gk876078
• gk861578
• gk835651
• gk601272
• gk874055
• gk519524
• gk493650
• gk617819
• gk543138
• gk570404
• ok1786
• gk182472
• gk182471
• gk182473
• WBVar01645908
• WBVar02080145
• gk955043
• gk958291

1 Chromosome

• WormBase ID: III
• Organism: Caenorhabditis elegans
• Length (nt): 13783801

1 Chromosome Location

• Feature . Primary Identifier: WBGene00006540
• Start: 9626536
• End: 9629154
• Strand: -1

4 Data Sets

• WormBaseAcedbConverter
• GO Annotation data set
• C. elegans genomic annotations (GFF3 Gene)
• Panther orthologue and paralogue predictions

1 Downstream Intergenic Region

• WormBase ID: intergenic_region_chrIII_9626530..9626535
• Length (nt): 6
• Chromosome Location: III: 9626530-9626535
• Organism: Caenorhabditis elegans

105 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons.	DESeq. False discovry rate (FDR) < 0.1.	WBPaper00048988:neuron_expressed
adult vs dauer larva	Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C.	N.A.	WBPaper00050488:adult_vs_dauer_regulated_N2_20C
Osmotic stress	Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when food was present	DESeq(version 1.10.1), FDR < 0.05.	WBPaper00050726:OsmoticStress_regulated_Food
	Transcripts that showed significantly increased expression after animals were treated with 50uM Rifampicin and 250uM Allantoin from day 1 to day 3 adult hermaphradite.	DESeq2(v1.14.1), fold change > 2, p-value < 0.05	WBPaper00055354:Rifampicin-Allantoin_upregulated
	Transcripts that showed significantly higher expression in somatic gonad precursor cells (SGP) vs. head mesodermal cells (hmc).	DESeq2, fold change >= 2, FDR <= 0.01.	WBPaper00056826:SGP_biased
	Transcripts that showed significantly increased expression after animals were treated with 100uM Psora and 250uM Allantoin from day 1 to day 3 adult hermaphradite.	DESeq2(v1.14.1), fold change > 2, p-value < 0.05	WBPaper00055354:Psora-Allantoin_upregulated
	Transcripts that showed significantly increased expression after animals were treated with 100uM Rapamycin and 50mM Metformin from day 1 to day 3 adult hermaphradite.	DESeq2(v1.14.1), fold change > 2, p-value < 0.05	WBPaper00055354:Rapamycin-Metformin_upregulated
	Proteins interacting with NHR-49-GFP according to co-IP and LC-MS.	N.A.	WBPaper00064071:NHR-49_interacting
	Maternal class (M): genes that are called present in at least one of the three PC6 replicates.	A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing.	[cgc5767]:expression_class_M
	Transcripts that showed significantly increased expression in day 1 adult hermaphrodite comparing to in L4 larva fem-3(q20) animals.	Fold change > 2, FDR < 0.05	WBPaper00064088:Day-1-adult_vs_L4_upregulated_fem-3(q20)
	Transcripts that showed significantly increased expression in day 3 adult hermaphrodite comparing to in L4 larva fem-3(q20) animals.	Fold change > 2, FDR < 0.05	WBPaper00064088:Day-3-adult_vs_L4_upregulated_fem-3(q20)
	Transcripts that showed significantly increased expression in 10-days post L4 adult hermaphrodite N2 grown at 20C, comparing to in 1-day post L4 adult hermaphrodite N2 animals grown at 20C.	CuffDiff, fold change > 2.	WBPaper00065096:Day10_vs_Day1_upregulated
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:A-class-motor-neurons_L2-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:all-neurons_L2-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:coelomocytes_L2-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L3/L4 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:dopaminergic-neurons_L3-L4-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:excretory-cell_L2-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:GABAergic-motor-neurons_L2-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (embryonic 0hr reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:germline-precursors_blastula-embryo_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:glr-1(+)-neurons_L2-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L3/L4 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:hypodermis_L3-L4-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:intestine_L2-larva_expressed
	Genes that showed expression levels higher than the corresponding reference sample (L3/L4 all cell reference).	A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes.	WBPaper00037950:PVD-OLL-neurons_L3-L4-larva_expressed
	Transcripts that showed significantly increased expression in ilc-17.1(syb5296) comparing to in N2 animals at L4 larva stage.	DESeq2, fold change > 2, FDR < 0.05.	WBPaper00066594:ilc-17.1(syb5296)_upregulated
	Proteins interacting with HA-PPM-1.D.	N.A.	WBPaper00062498:PPM-1.D_interacting
	Genes that were not enriched in either spermatogenic fem-3(q96gf) nor oogenic fog-2(q71) gonads, according to RNAseq analysis.	To identify differentially expressed transcripts, authors used R/Bioconductor package DESeq.	WBPaper00045521:Gender_Neutral
	Transcripts that showed altered expression from P0 to F2 generation animals after N2 parental generation were treated with antimycin, but not in damt-1(gk961032) P0 to F2 animals after the parenal generaton were treated with antimycin.	N.A.	WBPaper00055862:antimycin_damt-1(gk961032)_regulated
	Transcripts that showed significantly decreased expression in the neurons of bcat-1(RNAi) animals at 5-days post L4 adult hermaphrodite stage, comparing to animals injected with empty vector.	DESeq2. FDR < 0.05.	WBPaper00060459:bcat-1(RNAi)_downregulated
	Proteins identified in extracellular vesicle.	N.A.	WBPaper00062669:extracellular-vesicle_protein
	Transcripts that showed decreased expression in hlh-11(ko1) knockout strain comparing to in wild type background.	DESeq2, FDR < 0.05	WBPaper00060683:hlh-11(ko1)_downregulated

15 Expression Patterns

• Primary Identifier: Expr4690
• Remark: Reporter gene fusion type not specified.
• Pattern: It was observed that the fusion gene expression continued from embryonic and post-embryonic stages. At embryonic stage, several cells were stained in post-gastrulating embryos whereas in postembryonic stages staining of cells was seen from L1 to young adults.

• Primary Identifier: Expr4689
• Pattern: It was found that the amount of transcript of tbg-1 varies significantly in different stages during the development. In embryos the expression of the gene was high; it has extremely low level of gene expression during L1 larval stage, increased from L2 to L4 stages and showed the maximum expression in young adult stage. In gravid adult stage, the expression was more than that of embryos.

• Primary Identifier: Expr2035479
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr8293
• Remark: Picture: Fig. 7, Fig. 5, and Fig. S2.
• Subcellular Localization: Meiotic spindle assembly initiates at nuclear envelope breakdown, when a diffuse cloud of microtubules fills the volume of the perforated nucleus. These microtubules then organize into a much smaller assembly of dense microtubule bundles by the time the oocyte ovulates into the spermatheca. By the time the embryo exits the spermatheca and enters the uterus, a bipolar, metaphase-length spindle is present within a vesicle-free zone visible by differential interference contrast. Before NEBD, GFP::TBG-1 became progressively more concentrated on the nuclear envelope as oocyte maturation progressed. At nuclear envelope breakdown, GFP::TBG-1 fluorescence entered the nuclear region, forming a diffuse cloud that might indicate association with either microtubules or vesicles derived from the nuclear envelope. This large, diffuse area of gamma-tubulin enrichment did not contract in diameter nor increase in local intensity, as did the alpha-tubulin. By the time the embryo exited the spermatheca into the uterus, no discrete localization of GFP::TBG-1 was observed in the vesicle-free zone that contains the metaphase I spindle. GFP::MEI-1 was also distributed throughout the volume of the perforated nucleus immediately after nuclear envelope breakdown. GFP::MEI-1 differed from GFP::TBG-1 in that it became highly concentrated on chromosomes and spindle poles during spindle assembly and remained concentrated on these structures throughout meiosis.

• Primary Identifier: Expr1032689
• Pattern: Tiling arrays expression graphs

• Primary Identifier: Expr1102
• Remark: gamma-tubulin = tbg-1
• Subcellular Localization: gamma-Tubulin was detected at the centrosomes of mitotic germ cells localized at the distal tip of the gonad. In meiotic cells arrested at the pachytene stage in the middle of the gonad, g-tubulin staining of the centrosomes was more difficult to visualize. In oocytes arrested in diakinesis of prophase I in the proximal gonad, only a diffuse cytoplasmic g-tubulin staining was detected and no centrosomes. Immunostaining of mature oocytes failed to reveal any g-tubulin at the poles of both meiosis I and meiosis II spindles. The spermatheca of the hermaphrodite gonad and identified spermatocytes undergoing meiotic division was examed. g-Tubulin staining was detected at the poles of the spindles as focused dots corresponding to the centrosomes. Mature sperm cells were also observed, showing a weak dot staining at the position expected for the centrioles.

• Primary Identifier: Expr1152673
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015

• Primary Identifier: Expr12609
• Subcellular Localization: tbg-1 concentrated at hemidesmosomes and seam-seam adherens junctions.

• Primary Identifier: Expr2017343
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr1027294
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012

• Primary Identifier: Expr1645
• Remark: No detailed description for cellular expression pattern. Probably expressed everywhere. --wjc.
• Subcellular Localization: After affinity purification, the antibodies stained centrosomes brightly. In newly fertilized embryos, gamma-tubulin antibodies did not stain the maternal meiotic spindle, which is barrel shaped and lacks centrioles and asters. Centrosome staining was seen first as 2 small dots near the sperm pronucleus at the posterior pole. This is consistent with recruitment of maternal gamma-tubulin by sperm-supplied centrioles. The small dots of gamma-tubulin grew into bright spheres as the male and female pronuclei migrated to meet in the posterior hemisphere of the embryo. In later-stage embryos, gamma-tubulin antibodies stained tiny centrosomes in interphase cells and larger centrosomes in mitotic cells. Anti-gamma-tubulin immunofluorescence signal from centrosomes was reduced below detection in embryos from hermaphrodites injected with dsRNA made from the gamma-tubulin gene, confirming the specificity of the antibodies.

• Primary Identifier: Expr13320
• Pattern: tbg-1 encodes gamma-tubulin, a ubiquitous and highly conserved component of centrosomes in eukaryotic cells (Strome et al, 2001). Using smFISH we determined the localization of tbg-1 transcripts (red). tbg-1 transcripts are detected within distinct foci throughout the cytoplasm during both the first (A) and second (B) mitosis. tbg-1 transcripts are not enriched at centrosomes or either blastomere. In contrast, GFP tagged TBG-1 proteins (green signal; arrowheads) localize at centrosomes, as previously shown (Strome et al, 2001). Shown are projections from selected focal planes. Bar=10μm.

• Primary Identifier: Expr3451
• Subcellular Localization: During anaphase, the centrosomal spheres of gamma-tubulin became hollow, and 2 dots of gamma-tubulin appeared within each ring. After anaphase, the gamma-tubulin rings disappeared, and the dots grew into the next pair of centrosomes. Thus, the dots represent the recruitment of gamma-tubulin by separated and perhaps duplicated centrioles within the expanding and disassembling mother centrosome. The behavior of the posterior centrosome differed from that of the anterior centrosome during anaphase B spindle elongation. As described previously, unlike the anterior pole, which remained relatively stationary, the posterior pole swung from side to side and moved toward the posterior end of the embryo. After the posterior migration had ceased, the gamma-tubulin ring at the posterior pole flattened laterally, decreased in brightness, and appeared to fragment, as if subject to forces directed toward the lateral cortex. In contrast, the gamma-tubulin ring at the anterior pole persisted longer and showed little flattening or fragmentation as it decreased in brightness. In subsequent rounds of mitosis, this pattern of centrosomal gamma-tubulin dynamics was repeated on a progressively smaller scale.

• Primary Identifier: Expr13934
• Pattern: In non-ciliated neurons TBG-1 γ-tubulin is diffusely localized throughout the neuron and is only slightly enriched, at the dendrite tip.

• Primary Identifier: Expr13935
• Pattern: WBTransgene00025665
• Subcellular Localization: Ciliated neurons and URX show a consistent enrichment of TBG-1 γ-tubulin at the base of the cilium, as has been reported before (Bobinnec et al., 2000).

37 GO Annotation

10 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00006540
• Start: 9626536
• End: 9629154
• Strand: -1

37 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 2619

1 Sequence Ontology Term