WormMine: ExpressionPattern Expr1806

• Pattern: Consistent with the results from the transcriptional fusion, the expression observed near the top of the vulval lumen in animals carrying the translational fusion corresponds most closely to the position of vulE. The 3-D expression pattern of the EGL-26::GFP translational fusion protein showed expression in a ring around the ventral region of the vulva, in a thicker region near the center of the vulva, and at the apex of the vulva corresponding to where the utse lies separating the vulval and uterine lumens. The thick region of expression in the center of the vulva closely corresponds to the vulE cells. The ring around the ventral region of the vulval most likely corresponds to expression by vulB2 as assayed by the transcriptional fusion. In animals that carry this fusion construct on an extrachromosomal array, kuEx90, expression was observed in many regions of the animal, although not ubiquitously. Expression is strong around the cells of the spermatheca, around the mouth, and lining the pharynx, the rectum, and the excretory canals. Expression was also seen in the pharyngeal intestinal junction cells, transiently during L3 in the anchor cell, in rectal epithelial cells D, VL, and VR, in B and in Y, and in several cells with a neuronal appearance.
• Primary Identifier: Expr1806
• Remark: Because of the lack of nuclear or cytoplasmic expression, it was difficult to determine which cells were expressing egl-26::gfp in the animals carrying the translational fusion. Because pWH15 retained egl-26 activity as assayed by ability to rescue ku211 and ku228 mutants when expressed from an extrachromosomal array (2/4 lines rescued), it is assumed that the EGL-26::GFP expression pattern would accurately represent global and subcellular localization of EGL-26.
• Subcellular Localization: Expression is obvious near the vulva and the uterus only during L4. However, it is often very difficult to tell which cell is actually expressing egl-26::gfp because the fusion protein appears to line the lumen of the uterus and portions of the vulval lumen and is not obviously associated with any particular cell cytoplasm. Even in cases where a cell cytoplasm obviously contains egl-26::gfp, expression is often brighter around the apical edge of the cell.

12 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A group of six equivalent cells forms a tightly constructed `valve` that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine.	pharyngeal-intestinal valve	cardia	WBbt:0005767
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr.	vulE		WBbt:0006767
membranous cell attaches the uterus to the lateral epidermis (seam) and forms a thin laminar process dorsal to the vulva. formed by fusion of eight pi cell progeny and AC.	uterine seam cell	utse	WBbt:0006789
adult vulval toroid cell, B2.	vulB2		WBbt:0006764
Rectal cell, postembryonic blast cell in male	B cell	lineage name: ABprppppapa	WBbt:0003825
Rectal epithelial cells, adjacent to intestino-rectal valve, have microvilli	rect_VR	lineage name: ABprppppaap	WBbt:0004776
Rectal epithelial cells, adjacent to intestino-rectal valve, have microvilli	rect_D	lineage name: ABplpappppp	WBbt:0004788
Rectal epithelial cells, adjacent to intestino-rectal valve, have microvilli	rect_VL	lineage name: ABplppppaap	WBbt:0004787
cuticle-lined lumenal region surrounded by pharyngeal epithelium, situated at the anterior end of pharynx.	buccal cavity		WBbt:0005255
Rectal cell at hatching, becomes PDA in hermaphrodite, postembryonic blast cell in male	Y cell	lineage name: ABprpppaaaa	WBbt:0004578

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001193	egl-26	C23H3.1	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038