WormMine: ExpressionPattern Expr1901

• Pattern: The distribution of signal in transgenic worms was unique and highly restricted with respect to tissue and/or stage of development but did not correspond to the descendants of a particular branch of the cell lineage. Fluorescence was first detectable at the comma stage in cells that divided and appeared to migrate during the 2-fold and 3-fold stages. Neuronal staining was obvious from L1 onward and by early L4 was seen to occur in both the dorsal and ventral nerve chords. During this stage, a strong signal was noted in the developing vulva (most likely the vulE and/or vulF cells). By late L4 an intense GFP signal in the spermathecal valve as well as other vulval and/or uterine structures was evident. Expression in the uv1 and uv2 cells was suggested by the pattern of fluorescence around the vulva. However, the nuclear-localized reporter construct stained more nuclei than can be accounted for by expression in these cells alone. With this construct, nuclear localized signal was observed in all four nuclei of the syncytial spermathecal valve cell. Although GFP fluorescence was seen to be strongest in the late L4 and early adult for the spermathecal valve and vulval/uterine structures previously noted, it was seen to persist throughout adulthood. The M8 cell of the terminal bulb of the pharynx, all six cells of the pharyngeal-intestinal valve, and neuronal cell bodies within the metacorpus and around the isthmus of the pharynx also expressed gly-2p::GFP. At least 37 neurons with cell bodies lying next to the ventral nerve chord were positive for gly-2-directed reporter expression in the adult hermaphrodite, although with widely varying levels of staining. There was also GFP fluorescence present in other neurons associated with the preanal, dorso-rectal, and/or lumbar ganglia. In adult males, expression was similar in non-sexually dimorphic tissues and was also observed in axons that project into rays 2, 3, 5, 6, and either 8 or 9 of the copulatory bursa.
• Primary Identifier: Expr1901

37 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A group of six equivalent cells forms a tightly constructed `valve` that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine.	pharyngeal-intestinal valve	cardia	WBbt:0005767
A valve tissue that connects the spermatheca and the uterus of a hermaphrodite gonad.	spermathecal-uterine junction	sp-ut valve	WBbt:0006756
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
neuron of the pharyngeal nervous system.	pharyngeal neuron		WBbt:0005439
a bundle of nerve processes that runs along the dorsal mid-line of the animal.	dorsal nerve cord	dorsal cord	WBbt:0006750
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar.	vulF		WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr.	vulE		WBbt:0006767
uterine-vulval cell proximal to the vulva.	uv1		WBbt:0006791
Post-embryonic cell of pedigree V6L.papapaaa, male sensory rays, neuron, striated rootlet.	R2AL	lineage name: R2L.aaa	WBbt:0004042
Post-embryonic cell of pedigree V6R.papapaaa, male sensory rays, neuron, striated rootlet.	R2AR	lineage name: R2R.aaa	WBbt:0004041
eighth pharyngeal muscle cell layer (single cell)	pm8	lineage name: MSaaapapp	WBbt:0004302
uterine-vulval cell, medial position of three ut cells.	uv2		WBbt:0006792
Post-embryonic cell of pedigree TL.appapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R9BL	lineage name: TL.appapapa	WBbt:0003968
Male sensory rays, neuron, darkly staining tip, open to outside	R9BR	lineage name: TR.appapapa	WBbt:0003966
Male sensory rays, neuron, darkly staining tip, open to outside	R8BR	lineage name: TR.appaaapa	WBbt:0003988
Post-embryonic cell of pedigree V6L.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BL	lineage name: V6L.ppppaapa	WBbt:0004022
Post-embryonic cell of pedigree V6R.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BR	lineage name: V6R.ppppaapa	WBbt:0004021
Post-embryonic cell of pedigree V6L.papppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R3BL	lineage name: V6L.papppapa	WBbt:0004034
Post-embryonic cell of pedigree V6R.papppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R3BR	lineage name: V6R.papppapa	WBbt:0004033
Post-embryonic cell of pedigree TL.appaaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R8BL	lineage name: TL.appaaapa	WBbt:0003990
Post-embryonic cell of pedigree V6R.papapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R2BR	lineage name: V6R.papapapa	WBbt:0004039
Post-embryonic cell of pedigree V6L.papapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R2BL	lineage name: V6L.papapapa	WBbt:0004040
Post-embryonic cell of pedigree V6R.pppppapa, male sensory rays, neuron, not darkly staining nor open to outside.	R6BR	lineage name: V6R.pppppapa	WBbt:0004012
Post-embryonic cell of pedigree V6L.pppppaaa, male sensory rays, neuron, striated rootlet.	R6AL	lineage name: V6L.pppppaaa	WBbt:0004018
Post-embryonic cell of pedigree V6R.pppppaaa, male sensory rays, neuron, striated rootlet.	R6AR	lineage name: V6R.pppppaaa	WBbt:0004016
Post-embryonic cell of pedigree V6L.pppppapa, male sensory rays, neuron, not darkly staining nor open to outside.	R6BL	lineage name: V6L.pppppapa	WBbt:0004014
Male sensory rays, neuron, striated rootlet	R9AR	lineage name: TR.appapaaa	WBbt:0003970
Post-embryonic cell of pedigree V6R.ppppaaaa, male sensory rays, neuron, striated rootlet.	R5AR	lineage name: V6R.ppppaaaa	WBbt:0004023
Male sensory rays, neuron, striated rootlet	R9AL	lineage name: TL.appapaaa	WBbt:0003971
Post-embryonic cell of pedigree V6R.papppaaa, male sensory rays, neuron, striated rootlet.	R3AR	lineage name: V6R.papppaaa	WBbt:0004035

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001627	gly-2	C55B7.2	Caenorhabditis elegans

10 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018