WormMine: ExpressionPattern Expr8859

• Pattern: Both smf-1::GFP and SMF-1::GFP were prominently expressed in the anterior and posterior intestine and associated gland cells. A strong expression was observed in the anchor cell (AC) during larval life and in the adult proximal uterus resulting from the fusion of AC with uv1, uv2 and utse cells. GFP signal was also consistently seen in the adult spermatheca. Fainter expression was observed in the major epidermis hyp7, in the pharyngeal muscles, and in a subset of anterior sensory neurons, ring neurons, and posterior-head neurons. The GFP signal was detected from late embryogenesis to adult stage, with generally higher expression levels in young larvae (L1 stage).
• Primary Identifier: Expr8859
• Remark: Picture: Fig 6, Fig 7.
• Subcellular Localization: SMF-1::GFP and SMF-3::GFP were localized at the apical plasma membrane in all epithelia in which they were expressed.

14 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
Secretory gland cell of the pharynx	Pharyngeal gland cell		WBbt:0005788
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
	rectal gland cell		WBbt:0005799
uterine-vulval cell proximal to the vulva.	uv1		WBbt:0006791
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522
membranous cell attaches the uterus to the lateral epidermis (seam) and forms a thin laminar process dorsal to the vulva. formed by fusion of eight pi cell progeny and AC.	uterine seam cell	utse	WBbt:0006789
neuron that senses external stimuli and transduce them to signals internal to the organism.	sensory neuron		WBbt:0005759
uterine-vulval cell, medial position of three ut cells.	uv2		WBbt:0006792

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004876	smf-1	K11G12.4	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021