WormMine: ExpressionPattern Expr8560

• Pattern: At the same developmental stages, HSP43 staining was seen in desmosomes of cells which make up the spermathecal cage and the spermathecal valve. L2 larvae, which lack vulva and spermatheca, showed prominent HSP43 staining anteriorly in the pharyngealintestinal valve, a structure made up of six cells coupled by a system of desmosomes, and posteriorly in a discreet region probably corresponding to the intestinalrectal valve. In both control and heat-shocked adult and L4 larvae a low level of staining was seen throughout the body, together with very strong, specific, staining in the vulva and spermatheca. In the vulval region, HSP43 was localized in the adherens junctions of vulval epithelial cells, but absent from vulval muscle. Images taken in different focal planes of the vulval region revealed HSP43 staining in the utse cells and in the uv1 cells.
• Primary Identifier: Expr8560
• Remark: Picture: Figure 2. This pattern appears identical with that reported for monoclonal antibody MH5, which labels fibrous organelles containing hemidesmosomes associated with the hypodermis, where it contacts the muscle cells. Similar patterns, but differing in some details, were noted with monoclonal antibodies MH4 and MH46. The specificity of the antiserum for HSP43 is demonstrated by the loss of signal when the antibody is pre-incubated with excess pure recombinant HSP43.
• Subcellular Localization: In the vulval region, HSP43 was localized in the adherens junctions of vulval epithelial cells, In all post-embryonic stages, HSP43 staining was observed in a very regular punctate pattern over body-wall muscle, the columns of labelled spots running circumferentially along the muscle quadrant and the signal being more intense where the muscle cells contact each other. Individual bands could be resolved as doublets separated by a narrow space.

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
female genital.	vulva		WBbt:0006748
A group of six equivalent cells forms a tightly constructed `valve` that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine.	pharyngeal-intestinal valve	cardia	WBbt:0005767
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
uterine-vulval cell proximal to the vulva.	uv1		WBbt:0006791
membranous cell attaches the uterus to the lateral epidermis (seam) and forms a thin laminar process dorsal to the vulva. formed by fusion of eight pi cell progeny and AC.	uterine seam cell	utse	WBbt:0006789
Intestino-rectal valve	virL	lineage name: ABprpappppp	WBbt:0004511
Intestino-rectal valve	virR	lineage name: ABprpappppa	WBbt:0004510

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002024	hsp-43	C14F11.5	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038