WormMine: ExpressionPattern Expr12243

• Pattern: CRB- 3::GFP was first detected in embryonic pharyngeal and intestinal precursor cells. Throughout the larval stages the fusion protein localized to the apical membrane domain of pharyngeal cells, to the excretory canal, to the apical membrane domain of intestinal cells, to a circumferential pattern resembling the pattern of commissural axons, to neurons in the dorsal and ventral nerve cords, to the coelomocytes, and in a fraction of animals (n54/6) to the apical membrane domain of the rectal epithelium. During the fourth larval stage, CRB-3::GFP became visible in the uterus. No fusion protein was detected in the seam cells or vulval epithelial cells, two tissues in which EAT-20 was shown to be expressed (Shibata et al., 2000).
• Primary Identifier: Expr12243

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
	pharyngeal cell		WBbt:0005460
a bundle of nerve processes that runs along the dorsal mid-line of the animal.	dorsal nerve cord	dorsal cord	WBbt:0006750
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775
epithelium connecting intestine and anus.	rectal epithelium		WBbt:0005800
any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.	intestinal cell		WBbt:0005792

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00044743	crb-3	C35B8.4	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023