WormMine: ExpressionPattern Expr2245

• Pattern: NHX-3 appeared to be associated with intracellular membranes. NHX-3::GFP expression was identical in both translational and transcriptional fusions and occurred primarily in the hypodermal cells of the main body syncytium; in addition, in adult animals the uterine cells in the region closest to the vulva were intensely labeled, as were the spermathecal junction cells. The uterine cells are likely ut1, a toroidal cell proximal to the vulva. In addition, labeling was detected in socket cells and the excretory pore cell. The fluorescent expression pattern resembled dots distributed randomly throughout the cells. Under maximum resolution, these structures were seen to be elliptical, with circumferential labeling. In contrast to the usual suspects (Golgi, endoplasmic reticulum, endosomes, or mitochondria) these dots may represent storage granules, because hypodermal cells function as a major storage depot for granules and lipid droplets. Alternatively, the structures could be one of two other organelles that are unique to hypodermal cells, multivesicular bodies, or Ward bodies, which presumably play roles in the hypodermis-specific functions of secretion of cuticle components or the phagocytosis of apoptotic cells.
• Primary Identifier: Expr2245
• Remark: In general, the expression patterns found for the translational fusion constructs were similar to those reported above for the transcriptional fusions.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A valve tissue that connects the spermatheca and the uterus of a hermaphrodite gonad.	spermathecal-uterine junction	sp-ut valve	WBbt:0006756
An interfacial epithelial cell which forms a distal cap on a sheath cell to bind the sheath of a sensillum to the neighboring hypodermis, via adherens junctions. There is often a narrow opening through the socket from the exterior into the sheath channel, allowing ciliated dendrites to be exposed to the external environment. The socket cell functions similarly to a glial cell.	socket cell		WBbt:0005750
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
The outlet of the excretory sinus to the exterior environment through the cuticle, on the ventral side of the head, just behind the nerve ring.	excretory pore	pore	WBbt:0005778
uterine toroidal epithelial cell.	ut1		WBbt:0006785

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003731	nhx-3	C54F6.13	Caenorhabditis elegans

0 Life Stages