WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  At approximately 240 min post-fertilization, UNC-39::GFP appeared in more posteriorly located nuclei in addition to the anterior nuclei. Posterior nuclei expressing unc-39::gfp included Z1 and Z4, the M cell, and the embryonic coelomocytes. Occasionally expressed in additional posteriorly located cells. Expression in M and the coelomocytes was transient and no longer observed by 600 min post-fertilization, whereas Z1 and Z4 expression persisted into the L1 larval stage. Expression of the full-length unc-39::gfp transgene was dynamic throughout development. UNC-39::GFP was first seen at approximately 100 min post-fertilization when gastrulation and embryonic transcription begin. UNC-39::GFP accumulated in the nuclei of 12 to 16 blast cells in the anterior of the embryo. As embryogenesis proceeded, expression was observed in increasing numbers of anterior nuclei which were most likely the descendants of the earlier-expressing cells, as UNC-39::GFP-expressing pairs of cells in the process of cell division were observed. Expression in anterior nuclei continued until about 450 min post-fertilization (2-fold stage), at which time expression became restricted to less than 10 cells and persisted into adulthood. The locations and morphologies of some of these cells indicated that they were anterior neuroblasts derived from the AB lineage. In addition to expression in the anteriorly generated mesodermal Z1, Z4, M, and coelomocytes, the unc-39 promoter was active in all of the body wall muscle cells of late embryos and L1 larvae. Thus, unc-39::gfp transgenes were expressed very early in embryogenesis in anterior cells that generate neuronal and mesodermal tissues, and later in body wall muscle cells. The 4-kb unc-39 promoter driving the expression of gfp alone showed a similar general pattern. However, expression of this construct persisted in a greater number of anterior cells into larval and adult stages. These cells had axon and dendrite morphologies characteristic of amphid sensory neurons. Furthermore, the CAN neuron clearly showed unc-39 promoter expression. Primary Identifier  Expr3094

9 Anatomy Terms

Definition Name Synonym Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
A blast cell from which a neuron develops. neuroblast   WBbt:0008594
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles. coelomocyte   WBbt:0005751
neuron of the amphid sensillum amphid neuron amphid sensory neuron WBbt:0005394
Process runs along excretory canal, no synapses, essential for survival CANL lineage name: ABalapaaapa WBbt:0004949
Process runs along excretory canal, no synapses, essential for survival CANR lineage name: ABalappappa WBbt:0004947
Somatic gonad precursor cell Z4 lineage name: MSappaap WBbt:0004574
Somatic gonad precursor cell Z1 lineage name: MSpppaap WBbt:0004577
Postembryonic mesoblast of pedigree MSapaapp. M cell lineage name: MSapaapp WBbt:0004489

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00006775 unc-39 F56A12.1 Caenorhabditis elegans

6 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. embryo Ce WBls:0000003