WormMine: ExpressionPattern Expr12667

• Pattern: To characterize the expression of mnp-1, reporter analysis was performed using an mnp-1p-GFP transgene containing 3 kb of upstream sequence driving a nuclear localized green fluorescent protein (GFP). At the comma stage (approximately 400 cells) and persistent through hatching, GFP expression was apparent in all of the body-wall muscle cells. At these embryonic stages, GFP expression is also seen in the migrating z1 and z4 gonadal precursor cells and a number of unidentified cells in the dorsal anterior region. These unidentified cells are likely neuronal precursors based on their position, although other cells types are not ruled out. After hatching, GFP expression fades quickly and is typically absent by mid to late L1 larval stage. To characterize the expression of mnp-1, reporter analysis was performed using an mnp-1p::GFP transgene containing 3 kb of upstream sequence driving a nuclear localized green fluorescent protein (GFP). At the comma stage (approximately 400 cells) and persistent through hatching, GFP expression was apparent in all of the body-wall muscle cells. At these embryonic stages, GFP expression is also seen in the migrating z1 and z4 gonadal precursor cells and a number of unidentified cells in the dorsal anterior region. These unidentified cells are likely neuronal precursors based on their position, although other cells types are not ruled out. After hatching, GFP expression fades quickly and is typically absent by mid to late L1 larval stage.
• Primary Identifier: Expr12667
• Subcellular Localization: Expression clearly localized GFP proximal to the plasma membrane of embryonic muscle cells, suggesting that the portion of MNP-1 containing the predicted transmembrane domain is sufficient to direct localization to the plasma membrane.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
A blast cell from which a neuron develops.	neuroblast		WBbt:0008594
Somatic gonad precursor cell	Z4	lineage name: MSappaap	WBbt:0004574
Somatic gonad precursor cell	Z1	lineage name: MSpppaap	WBbt:0004577

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007139	mnp-1	B0285.7	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018