WormMine: ExpressionPattern Expr1500

• Pattern: Both: The transgene constructs showed minimal expression in non-neuronal cells. However, transgene expression in intestinal, hypodermal, or muscle cells was observed in occasional transgenic lines in which 3.0-kb genomic DNA fragments extending just past the CePPEF start codon directed production of short N-terminal regions of CePPEF fused to GFP. As these expression patterns occurred sporadically, they likely reflect transgene effects rather than an expression pattern relevant to the endogenous CePPEF gene. FL-GFP: FL-GFP transgene directed GFP expression to the same set of neurons as the NLS-GFP-LacZ construct, and expression in a single posterior neuron was also more clearly observed. Within the expressing cells, the FL-GFP fusion protein localized efficiently to several structures beyond the cell soma, including axons that form the ventral nerve cord; dendrites that extend to the anterior tip of the animal; and the cilia of neurons AWB, AWC, and BAG. aa-(1)-NLS-GFP-LacZ: Injection of the aa-(1)-NLS-GFP-LacZ construct into C. elegans revealed reporter gene expression in several anterior neurons, including AWB, AWC, AVA, AVB, AVX, BAG, and URX. The ASE neuron showed inconsistent transgene expression.
• Primary Identifier: Expr1500
• Subcellular Localization: FL-GFP: Within the expressing cells, the FL-GFP fusion protein localized efficiently to several structures beyond the cell soma, including axons that form the ventral nerve cord; dendrites that extend to the anterior tip of the animal; and the cilia of neurons AWB, AWC, and BAG. aa-(1)-NLS-GFP-LacZ: GFP-LacZ fusion protein facilitates retention in the nucleus.

18 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Ring interneuron	URXL	lineage name: ABplaaaaappp	WBbt:0004928
Ring interneuron	URXR	lineage name: ABarpapaappp	WBbt:0004926
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASEL	lineage name: ABalppppppaa	WBbt:0003904
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASER	lineage name: ABpraaapppaa	WBbt:0003903
Neuron, ciliated ending in head, no supporting cells, associated with ILso	BAGL	lineage name: ABalppappap	WBbt:0003824
Neuron, ciliated ending in head, no supporting cells, associated with ILso	BAGR	lineage name: ABarappppap	WBbt:0003823
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBR	lineage name: ABpraaappap	WBbt:0003828
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBL	lineage name: ABalpppppap	WBbt:0003829
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWCL	lineage name: ABplpaaaaap	WBbt:0003827
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWCR	lineage name: ABprpaaaaap	WBbt:0003826
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAL	lineage name: ABalppaaapa	WBbt:0003870
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAR	lineage name: ABalaappapa	WBbt:0003869
Ventral cord interneuron, synapses onto VB and DB motor neurons; formerly called beta.	AVBR	lineage name: ABprpaapaap	WBbt:0003867
Ventral cord interneuron, synapses onto VB and DB motor neurons; formerly called beta.	AVBL	lineage name: ABplpaapaap	WBbt:0003868
any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.	intestinal cell		WBbt:0005792

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003969	pef-1	F23H11.8	Caenorhabditis elegans

0 Life Stages