WormMine: ExpressionPattern Expr10016

• Pattern: The clp-1 reporter was active in neurons and colocalized with both the pan-neural unc-119::gfp and the GABAergic unc-47::gfp reporters. In addition, the clp-1 reporter was expressed in cells of the ventral and dorsal nerve cords. We co-expressed the clp-1 reporter with the body wall muscle marker myo-3p::gfp::nls and found that was active in muscle. clp-1 transcriptional reporter was also detected in other tissues, including the intestine, vulva, the head-pharyngeal muscles and the nerve ring. The expression pattern remained unchanged over the course of larval development through to adulthood.
• Primary Identifier: Expr10016
• Subcellular Localization: CLP-1::mRFP was excluded from the nucleus and dense bodies, but was present at structures immediately adjacent to dense bodies. More specifically, CLP-1::mRFP co-localized with PAT-3::GFP at M-lines extending over the H-zone and at adhesion plaques. A native clp-1::gfp translational reporter confirmed that CLP-1::GFP displayed the same pattern of sarcomeric localization, as CLP-1::mRFP driven from the unc-54 promoter; CLP-1::GFP was also detected in other non-muscle tissues.

10 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
female genital.	vulva		WBbt:0006748
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
a bundle of nerve processes that runs along the dorsal mid-line of the animal.	dorsal nerve cord	dorsal cord	WBbt:0006750
neuron that uses GABA as a vesicular neurotransmitter.	GABAergic neuron		WBbt:0005190

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000542	clp-1	C06G4.2	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035