The longest construct, containing 10 kb of 5 upstream region from the first lin-3 exon, expresses lin-3::gfp in pharynx; spermathecal-uterine junction core cells and later in the spermatheca valve; pre-anchor (AC)/ventral uterine precursor (VU) cells and later in the anchor cell in the somatic gonad; vulF cells of the primary vulval lineage cells; and F, U and some of the B progeny cells in the male tail. This expression pattern was not affected by the different genetic backgrounds (dpy-20, pha-1 and unc-119) rescued by the corresponding co-injected rescue plasmids, implying that the gfp expression pattern is established by the lin-3 regulatory region. LIN-3 expression in different cells was temporally distinct as well. Expression in the pharynx was observed throughout post-embryonic stages. Spermathecal-uterine junction core cells, which later form the spermatheca valve, started expressing lin-3::gfp at the late L3 larval stage.
Primary Identifier
Expr2894
Remark
Reporter gene fusion type not specified.
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9 Anatomy Terms
Definition
Name
Synonym
Primary Identifier
A valve tissue that connects the spermatheca and the uterus of a hermaphrodite gonad.
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.