WormMine: ExpressionPattern Expr9038

• Pattern: The expression of nhr-154 GFP reporter transgenes was first detected in the 2-fold stage embryo within the developing pharynx and in precursors of several unidentified head neurons. By the 3-fold stage of embryogenesis, expression was seen in the pharynx and throughout the intestine, a pattern reminiscent of the developmental transcription factor pha-4. In L1 and L2 stages, the reporter gene expression was strong in the pharyngeal muscles (anterior and posterior bulbs, predominantly), in unidentified head neurons, the intestine, and in the intestinal-rectal valve or sphincter cell. GFP reporter gene expression decreased in subsequent larval stages (L3, L4) so that by the adult stage only pharyngeal expression persistence is reproducibly observed. This suggested that the peak of expression in adults detected by RT-qPCR might be due to the embryos inside gravid adults in these samples. No obvious differences in pattern were seen between the two promoter lengths tested. The expression of nhr-154 GFP reporter was not observed in male specific structures.
• Primary Identifier: Expr9038
• Remark: Picture: Fig 5A, 5B, 5C.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
a saddle-shaped muscle cell that encircles the intestinal-rectal valve	anal sphincter muscle	lineage name: ABprpppppap	WBbt:0005798
Intestino-rectal valve	virL	lineage name: ABprpappppp	WBbt:0004511
Intestino-rectal valve	virR	lineage name: ABprpappppa	WBbt:0004510

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007547	nhr-154	C13C4.2	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019