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Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  Almost every transgenic animal shows strong DAF-3/GFP expression in many, but not all, head neurons, the ventral nerve cord (both cell bodies and processes), the intestinal cells, especially the membrane adjacent to the intestinal lumen, and tail hypodermal cells and neurons. Weak expression in the pharynx, hypodermal V blast cells, P blast cells and hyp7 hypodermal cells is observed in about half of the transgenic animals. Expression in the tail hypodermal cells hyp 9, hyp 10, and hyp 11 is clearly seen in nearly every animal. This apparent difference between tail hypodermal expression and main body expression may be a consequence of the anatomy of the animal. The main body hypodermis is underlain by bright GFP in the intestine and ventral nerve cord, so weak expression in the hypodermis is hard to see against this background. Expression is rarely detected in dorsal body wall muscle. DAF-3/GFP is expressed in the distal tip cells and in their precursors, Z1.a and Z4.p, throughout development. DAF-3/GFP is also expressed strongly in unidentified vulval cells in adults. In wild-type embryos of 200 to 400 cells, DAF-3/GFP is expressed uniformly thoughout the embryo. Primary Identifier  Expr1455
Subcellular Localization  In wild type, DAF-3/GFP is primarily, although not exclusively, cytoplasmic. DAF-3/GFP subcellular distribution was examined in head neurons in the vicinity of ASI (the cell that produces the DAF-7 signal), as well as in intestinal cells. DAF-3/GFP was predominantly cytoplasmic in all animals. However, in all animals, dim GFP fluorescence was seen in the nucleus of some of the cells with bright cytoplasmic fluorescence, and in 25% of the animals, equivalent DAF-3/GFP levels in the nucleus and cytoplasm was observed in one or more cells.
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46 Anatomy Terms

Definition Name Synonym Primary Identifier
neuron with cell body associated with the ventral nerve cord. ventral cord neuron ventral cord motoneuron WBbt:0005300
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine. pharynx esophagus WBbt:0003681
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad posterior distal tip cell gon herm dtc P WBbt:0004506
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad anterior distal tip cell gon herm dtc A WBbt:0004520
neuron with its cell body situated in the head, excluding the pharynx. head neuron   WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum. tail neuron   WBbt:0006759
entire syncytium of hyp7 hyp7 syncytium hyp7 WBbt:0005734
Tail ventral hypodermis hyp10 lineage name: ABplppppppp WBbt:0004378
Tail ventral hypodermis, 9th hypodermis cell hyp9   WBbt:0004380
Tail dorsal hypodermis hyp11 lineage name: Cpappv WBbt:0004377
The tenth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P10   WBbt:0006779
The ninth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P9   WBbt:0006778
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1 P11 lineage name: ABplapappa WBbt:0004410
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1 P12 lineage name: ABprapappa WBbt:0004409
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar. vulF   WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr. vulE   WBbt:0006767
cell, posterior descendent of Z4, in hermaphrodite. Z4.p hermaphrodite   WBbt:0007024
cell, anterior descendent of Z1, in hermaphrodite. Z1.a hermaphrodite   WBbt:0007022
The sixth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P6   WBbt:0006775
The fifth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P5   WBbt:0006774
The fourth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P4   WBbt:0006773
The third (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P3   WBbt:0006772
The eighth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P8   WBbt:0006777
The seventh (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P7   WBbt:0006776
The second (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P2   WBbt:0006771
The first (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P1 P1 post-embryonic blast cell WBbt:0006770
adult vulval toroid cell, C, formed by fusion of precursors of lineage names P5.ppal, P5.ppar, P7.papl and P7.papr. vulC   WBbt:0006765
adult vulval toroid cell, B1, formed by fusion of precursors of lineage names P5.papa and P7.ppap. vulB1   WBbt:0006763

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000899 daf-3 F25E2.5 Caenorhabditis elegans

1 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  All C. elegans development stages, including embryo, larva and adult stage. all stages Ce WBls:0000002