WormMine: ExpressionPattern Expr2634

• Pattern: During development fluorescence was detected as early as the one-fold embryo stage, and was much more intense before hatching than for ace-1 and ace-2 at comparable embryonic stages. Although the full pattern was established as soon as the L1 stage, the fluorescence intensity decreased during development, especially in body-wall muscles, where it was often no longer detectable in adults. Only one set of body-wall muscles expressed GFP. These muscle cells were distributed dorsally in a double row. There was strong GFP expression in the pharynx at all larval stages and in adults. Staining identified 3 pm3, 3 pm4, 3 pm5 and 3 pm7 cells. pm6 were constantly negative as well as pm1, pm2 and pm8 cells. Neurons around the top of the isthmus and the base of pm5 cells were not identified. Two neurons of the preanal and lumbar ganglia were labelled and tentatively identified as PDA and PQR. In adult hermaphrodites, there was strong fluorescent staining of muscle cells of the pharynx, of a few neurons in the head and anal ganglion and in the two medial CAN cells (canal associated neurons). In routine histochemical reaction conditions, there is no staining for AChE activity corresponding to ace-3;ace-4 in double mutants ace-1;ace-2. Using higher substrate concentrations and longer incubation times, it was possible to detect weak staining (from ACE-3) in the pharynx and vulva region.
• Primary Identifier: Expr2634
• Remark: Reporter gene fusion type not specified.

12 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Neuron class of one sensory neuron, basal body, not part of a sensillum, projects into preanal gangion.	PQR	lineage name: QL.ap	WBbt:0004096
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
seventh pharyngeal muscle cell layer	pm7	m7	WBbt:0003721
Process runs along excretory canal, no synapses, essential for survival	CANL	lineage name: ABalapaaapa	WBbt:0004949
Process runs along excretory canal, no synapses, essential for survival	CANR	lineage name: ABalappappa	WBbt:0004947
third pharyngeal muscle cell layer	pm3	M3	WBbt:0003740
fifth pharyngeal muscle cell layer	pm5	M5	WBbt:0003737
fourth pharyngeal muscle cell layer	pm4	M4	WBbt:0003739
Neuron class of one motor neuron, process in dorsal cord, same as Y cell in hermaphrodite, Y.a in male.	PDA		WBbt:0004386

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000037	ace-3	Y48B6A.8	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019