WormMine: ExpressionPattern Expr661

• Pattern: lin-14 protein is first observed in embryos at ~7 hours after fertilization where most intense staining is seen in intestinal and hypodermal nuclei. ~9 h after fertilization, additional weak staining is observed. lin-14 protein is expressed at high level in the nuclei of most of the post-embryonic blast cells. Intense nuclear staining was observed in the hypodermal blast cells H1, H2, V1-V6 and T and in all of the intestinal (E) cells and weaker staining was observed in both neuroblasts Q1 and Q2, in the mesoblast M cell and in P cells (P1/2, P3/4 and P5/6). During L1, staining is seen in the progeny of the hypodermal blast cells H1, H2 V1-V6 and T and in all of the intestinal (E) cells. Staining in P-cell nuclei fades before migration into the ventral nerve cord but reappears later in some of their progeny cells. The embryo-derived nuclei in the hypodermal syncytial cell hyp7, ABarpppapa, ABplaapppp, Cpaaaa, Cpaapa, Cpaapp, Cpapaa, all stain for the lin-14 protein during the L1 stage. Terminally differentiated nuclei from embryonic body muscle also accumulate the lin-14 protein. Nuclei of many but not all neuronal cells stain with the antibody (e.g. BDU, ALM, CAN but not HSN). All of the embryonically generated ventral cord neurons and some but not all of the neurons of the nerve ring and the posterior ganglion accumulate the lin-14 protein in their nuclei during the L1 stage. Late L1 stage, staining is seen in all nuclei except in the neuronal nuclei staining is much weaker. In addition, more neurons of the nerve ring and posterior ganglion stain than at the earlier stages. Thus, in the hypodermal and intestinal cell lineages, lin-14 protein level peaks during early L1 and fade entirely by L2. In the many neuronal cells, lin-14 protein peak during mid to late L1 and fade by L2. Pn.p accumulates lin-14 protein at the L3 stage, although, very weak staining is seen before the Pn.p cells divide. This staining fades by early L4, In occasional L2 and L3 stage animals, weak staining is observed in nuclei and cytoplasm of hypodermal, neuronal and intestinal cells. Patches of staining in hypodermal or intestinal nuclei is only rarely observed in very old adults. In most adults, staining reappears only in the mature oocyte nuclei of hermaphrodites at meiotic prophase I when the chromosomes are condensed. The oocyte nuclear staining disappears after fertilization. Quantitation of immunoblots show that the level of lin-14 protein relative to a pharyngeal myosin control decreases >= 25-fold from L1 to L2.
• Primary Identifier: Expr661
• Remark: Lineage expression: H, V, T descandents. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).

57 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite.	P5.p hermaphrodite		WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite.	P4.p hermaphrodite		WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite.	P3.p hermaphrodite		WBbt:0008112
	P8.p hermaphrodite		WBbt:0008133
	P7.p hermaphrodite		WBbt:0008129
	P6.p hermaphrodite		WBbt:0008125
Posterior descendent cell of P1 fuses into postembryonic large hypodermal syncytium hyp7.	P1.p		WBbt:0006889
descendent cell of P11, posterior	P11.p		WBbt:0006899
descendent cell of P10, posterior	P10.p		WBbt:0006898
Posterior descendent cell of P9 fuses into postembryonic large hypodermal syncytium hyp7.	P9.p		WBbt:0006897
Posterior descendent cell of P2 fuses into postembryonic large hypodermal syncytium hyp7.	P2.p		WBbt:0006890
descendent cell of P12, posterior	P12.p		WBbt:0006900
Seam hypodermal cell, postembryonic blast cell	H1L	lineage name: ABplaaappp	WBbt:0004766
Seam hypodermal cell, postembryonic blast cell	H1R	lineage name: ABarpapppp	WBbt:0004765
Seam hyopdermal cell, postemb. blast cell, L1 deirid socket	H2L	lineage name: ABarppaaap	WBbt:0004764
Seam hyopdermal cell, postemb. blast cell, L1 deirid socket	H2R	lineage name: ABarpppaap	WBbt:0004763
Anterior lateral microtubule cell, touch receptor	ALML	lineage name: ABarppaappa	WBbt:0003954
Anterior lateral microtubule cell, touch receptor	ALMR	lineage name: ABarpppappa	WBbt:0003953
Process runs along excretory canal, no synapses, essential for survival	CANL	lineage name: ABalapaaapa	WBbt:0004949
Process runs along excretory canal, no synapses, essential for survival	CANR	lineage name: ABalappappa	WBbt:0004947
Neuron, process runs along excretory canal and into ring, unique darkly staining synaptic vesicles	BDUL	lineage name: ABarppaappp	WBbt:0003812
Neuron, process runs along excretory canal and into ring, unique darkly staining synaptic vesicles	BDUR	lineage name: ABarpppappp	WBbt:0003811
The sixth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P6		WBbt:0006775
The fifth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P5		WBbt:0006774

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003003	lin-14	T25C12.1	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003