WormMine: ExpressionPattern Expr8965

• Pattern: ELP-1::GFP was associated with the nine pairs of rays of the male tale. The relatively diffuse and broad band of fluorescence appears to originate from the hypodermal cells (hyp7) and one or more of the neuronal cells of the ray (RnA, RnB). In embryos, ELP-1::GFP expression first appeared during the comma stage. As the embryo matured into the 1-1.5 fold stage, the strongest expression was seen in the hypodermal cells, with only diffuse staining throughout the rest of the embryo. During larval development, expression of the fusion protein was progressively refined to muscle, neurons and epithelial cells. In the adult, ELP-1::GFP and ELP-1(truncated)::NLS::GFP were expressed in body wall muscle, spermatheca, vulval muscle, seam cells, the intestine, touch receptor neurons (TRNs) and inner labial 1 (IL1) neurons of the head.
• Primary Identifier: Expr8965
• Remark: Picture: Fig 3, 4, 5.
• Subcellular Localization: At the subcellular level, ELP-1::GFP was associated with adhesion sites in both males and hermaphrodites. In the hermaphrodite body wall muscle, ELP-1::GFP was associated with the muscle arms that terminate in neuromuscular junctions. The ELP-1::GFP fluorescence is found throughout the muscle arm. In males, ELP-1::GFP associated with repeating focal attachment points in the sex-specific muscles of the worm. ELP-1 expression was prominent at the adhesion sites located at the sarcolemma.

19 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
Inner labial neuron	IL1VR	lineage name: ABarapppppaa	WBbt:0004547
Inner labial neuron	IL1DR	lineage name: ABalappppaaa	WBbt:0004737
Inner labial neuron	IL1L	lineage name: ABalapaappaa	WBbt:0004553
Inner labial neuron	IL1R	lineage name: ABalaappppaa	WBbt:0004551
Inner labial neuron	IL1VL	lineage name: ABalppapppaa	WBbt:0004549
Inner labial neuron	IL1DL	lineage name: ABalapappaaa	WBbt:0004738
Neuron class of one neuron, posterior ventral microtuble cell, touch receptor.	PVM	lineage name: QL.paa	WBbt:0004086
Anterior lateral microtubule cell, touch receptor	ALML	lineage name: ABarppaappa	WBbt:0003954
Anterior lateral microtubule cell, touch receptor	ALMR	lineage name: ABarpppappa	WBbt:0003953
Neuron class of one sensory neuron, anterior ventral microtubule cell, touch receptor.	AVM	lineage name: QR.paa	WBbt:0003832
Posterior lateral microtubule cell, touch receptor	PLML	lineage name: ABplapappppaa	WBbt:0004104
Posterior lateral microtubule cell, touch receptor	PLMR	lineage name: ABprapappppaa	WBbt:0004103
male sensory organ; nine bilateral pairs of finger-like rays (numbered 1 to 9, Left/Right) radiate from the tail and are embedded in a cuticlar fan.	ray	male sensory ray	WBbt:0006941
primary cell type that forms the hypodermis.	hypodermal cell		WBbt:0007846

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001248	elp-1	F38A6.2	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015