WormMine: ExpressionPattern Expr8784

• Pattern: unc-62::gfp expression was first detectable during mid-embryogenesis in a subset of cells that include AB lineage derivatives. In newly hatched L1 larvae, unc-62::gfp was observed in multiple cell types including the M mesoblast, as well as a few neurons in the head and tail, and dorsal and ventral hypodermis. In the L1 larva, unc-62::gfp expression continued throughout the M lineage in all dividing M descendants. The GFP signal was transiently detectable in M-derived CCs and BWMs before they terminally differentiate, but remained detectable in the SMs and throughout the SM lineage, including the differentiated vulval muscles. Outside of the M lineage, the neuronal expression in the head and tail was retained throughout postembryonic development, while hypodermal expression appeared more variable. unc-62::gfp expression was also observed in a subset of ventral nerve cord (VNC) cells after the L2 stage and in the P lineage with initial faint GFP expression in P3.p, P4.p and P5.p in L1 animals, which expanded to P6.p, P7.p and P8.p cells at the L2 stage. Upon vulval induction, L3 and L4 larvae displayed strong GFP expression in P5.p, P6.p and P7.p and their descendants, but expression in P3.p, P4.p and P8.p and their descendants became weaker and eventually undetectable by mid-L4 stage. unc-62::gfp expression persisted in all differentiated vulval cells after vulval morphogenesis in adults.
• Primary Identifier: Expr8784
• Remark: Picture: Fig 4.

12 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite.	P5.p hermaphrodite		WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite.	P4.p hermaphrodite		WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite.	P3.p hermaphrodite		WBbt:0008112
	P8.p hermaphrodite		WBbt:0008133
	P7.p hermaphrodite		WBbt:0008129
	P6.p hermaphrodite		WBbt:0008125

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006796	unc-62	T28F12.2	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015