WormMine: ExpressionPattern Expr8775

• Pattern: The expression of the gfp::gstk-1 transgene was first detected in 100 cell embryos. Expression was increased during morphogenesis of the embryo. Fluorescence was observed as strong punctate staining in intestinal cells and in the epidermis. The number and intensity of fluorescent structures increased strongly in the intestine during larval development, whereas the epidermal fluorescent punctata weakened. In addition, a diffuse localization of GFP::GSTK-1 was observed in the body wall muscles and in the rectal gland cells in larvae and adults.
• Primary Identifier: Expr8775
• Remark: Picture: Fig 2, Fig. 3.
• Subcellular Localization: The presence of a peroxisomal targeting signal in its C-terminus and the punctate localization pattern suggest that GSTK-1 is a peroxisomal protein. To confirm the peroxisomal localization, the peroxisomal targeting signal (PTS1) was masked by fusing the GFP at the C-terminus of GSTK-1. Transgenic worms for GSTK-1::GFP only presented diffuse staining, further supporting a peroxisomal localization of GSTK-1. To confirm mitochondrial localization, both GFP::GSTK-1 and GSTK-2::GFP-expressing worms were stained with MitoTracker Red. GFP::GSTK-1 did not show any colocalization with MitoTracker Red staining.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
	rectal gland cell		WBbt:0005799

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00014251	gstk-1	ZK1320.1	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010