WormMine: ExpressionPattern Expr1681

• Pattern: During larval stages, ceh-43::gfp diminishes and is almost absent in adults. In newly hatched larvae, we identified the binucleate dorsal hyp3 and ventral hyp4 cells, the CAN neurons, and the PVQ neurons. Sheath and/or socket cells of several other anterior sensilla are stained as well, identified from their morphology and their position around the pharynx procorpus. In 3-fold stage embryos that move around in the eggshell, the mouth opening can occasionally be seen from above; at the tip of the head, a small ring encircles the mouth opening. It most likely belongs to a small cylindrical hypodermal syncytium such as hyp4 or hyp3, or other small hypodermal cells that connect the buccal cavity to the mouth. All the identified and suspected cells belong to the AB lineage. ceh-43::gfp expression starts around gastrulation in several superficial cells. By following cell divisions, almost all fifth generation descendants of the AB blastomere were identified as GFP positive. Shortly before morphogenesis, GFP expressing cells lie anteriorly, laterally, and anterodorsally at the surface of the embryo. These positions are characteristic for clones of AB descendants. Not all the progeny of the AB blastomere express GFP. At 1.5-fold egg length, most embryonic cell divisions have occurred. At that time, GFP expression is strongest in cells surrounding an indentation at the anterior end, the anterior sensory depression, as well as in two superficial bilateral cells and one ventral cell in the tail. Among these cells are most likely the cells identified in larvae or their precursors. GFP expression is weaker in lateral head regions, the position of future nerve ring neurons.
• Primary Identifier: Expr1681
• Remark: During all developmental stages, ceh-43::gfp expression resembles the antibody staining pattern published by Panganiban et al. (1997). Therefore, both methods are likely to reflect the natural ceh-43 expression pattern. However, gfp expression is visible generally in fewer cells and is absent from most nerve ring neurons. Therefore, the reporter may not show the complete expression pattern. The short pDllB promoter drives expression only in the main body syncytium, hyp7, where pDllA is not expressed. Authors believe that this expression may not reflect the natural ceh-43::gfp expression, because it is not consistent with the antibody staining from Panganiban et al. (1997). Reporter gene fusion type not specified.

23 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a structural (glial) cell which forms an specialized environment surrounding the sensory ending(s) of one or more neurons; sometimes accompanied by a more distal socket cell. In early development of the sensory nerves and of the nerve ring some sheath cells (cephalics and labials) may also provide a substrate for axon guidance	neuronal sheath cell	pocket cell	WBbt:0005811
Cylindrical hypodermal syncytium in head	hyp3	lineage name: ABplaapaaaa	WBbt:0004690
Cylindrical hypodermal syncytium in head	hyp4	lineage name: ABarpapapa	WBbt:0004687
Interneuron, projects along ventral cord to ring	PVQL	lineage name: ABplapppaaa	WBbt:0004076
Interneuron, projects along ventral cord to ring	PVQR	lineage name: ABprapppaaa	WBbt:0004074
Process runs along excretory canal, no synapses, essential for survival	CANL	lineage name: ABalapaaapa	WBbt:0004949
Process runs along excretory canal, no synapses, essential for survival	CANR	lineage name: ABalappappa	WBbt:0004947
embryonic cell	ABarpp		WBbt:0006046
embryonic cell	ABalpp		WBbt:0006486
embryonic cell	ABplap		WBbt:0006096
embryonic cell	ABalap		WBbt:0006101
embryonic cell	ABarap		WBbt:0005903
embryonic cell	ABprpa		WBbt:0005915
embryonic cell	ABplpa		WBbt:0005950
embryonic cell	ABprap		WBbt:0005922
embryonic cell	ABprpp		WBbt:0006347
embryonic cell	ABaraa		WBbt:0006031
embryonic cell	ABplpp		WBbt:0006353
embryonic cell	ABpraa		WBbt:0006007
embryonic cell	ABalpa		WBbt:0006083
embryonic cell	ABplaa		WBbt:0006121
embryonic cell	ABarpa		WBbt:0006008
embryonic cell	ABalaa		WBbt:0006039

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000463	ceh-43	C28A5.4	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	A developemental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until death.		postembryonic Ce	WBls:0000022