WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00004751 Gene Name  sea-2
Sequence Name  ? K10G6.3 Brief Description  sea-2 encodes a zinc finger protein; SEA-2 is an autosomal signal element (ASE) that functions to interpret the X:A chromosomal ratio; in addition, SEA-2 functions autonomously in the intestine to regulate adult lifespan and autonomously in seam cells to regulate larval developmental timing; in regulating lifespan and developmental timing, SEA-2 functions as a post-transcriptional negative regulator of LIN-28, which specifies the L2 larval developmental program; sea-2 also interacts with the daf-2/insulin-IGF-1 signaling pathway to regulate larval developmental timing and adult lifespan; a SEA-2::GFP fusion protein is expressed in a variety of tissues, including seam cells, intestinal cells, pharyngeal muscles and nerve ring neurons, and localizes diffusely to the cytoplasm and the nucleus.
Organism  Caenorhabditis elegans Automated Description  Enables double-stranded RNA binding activity and single-stranded RNA binding activity. Involved in several processes, including determination of adult lifespan; regulation of gene expression; and response to heat. Located in cytoplasm and nucleus. Expressed in intestinal cell; nerve ring neurons; neurons; pharyngeal muscle cell; and seam cell.
Biotype  SO:0001217 Genetic Position  II :-5.94359 ±0.012241
Length (nt)  ? 21764
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00004751

Genomics

19 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:K10G6.3f.1 K10G6.3f.1 6459   II: 3950377-3972137
Transcript:K10G6.3h.1 K10G6.3h.1 5043   II: 3950377-3970060
Transcript:K10G6.3g.1 K10G6.3g.1 6409   II: 3950377-3972046
Transcript:K10G6.3i.1 K10G6.3i.1 5978   II: 3950377-3972050
Transcript:K10G6.3j.1 K10G6.3j.1 4755   II: 3962223-3970060
Transcript:K10G6.3b.1 K10G6.3b.1 5166   II: 3962223-3971544
Transcript:K10G6.3a.1 K10G6.3a.1 6212   II: 3962223-3972137
Transcript:K10G6.3c.1 K10G6.3c.1 6174   II: 3962223-3972140
Transcript:K10G6.3k.1 K10G6.3k.1 4950   II: 3962522-3970060
Transcript:K10G6.3l.1 K10G6.3l.1 4515   II: 3962522-3970060
Transcript:K10G6.3n.1 K10G6.3n.1 5379   II: 3962522-3971544
Transcript:K10G6.3o.1 K10G6.3o.1 4944   II: 3962522-3971544
Transcript:K10G6.3d.1 K10G6.3d.1 4464   II: 3964340-3970060
Transcript:K10G6.3m.1 K10G6.3m.1 4029   II: 3964340-3970060
Transcript:K10G6.3q.1 K10G6.3q.1 4458   II: 3964340-3971544
Transcript:K10G6.3r.1 K10G6.3r.1 4893   II: 3964340-3971544
Transcript:K10G6.3e.1 K10G6.3e.1 2877   II: 3966495-3970060
Transcript:K10G6.3s.1 K10G6.3s.1 3306   II: 3966495-3971544
Transcript:K10G6.3p.1 K10G6.3p.1 351   II: 3970098-3971544
 

Other

19 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:K10G6.3f K10G6.3f 5478   II: 3950377-3950396
CDS:K10G6.3h K10G6.3h 5043   II: 3950377-3950396
CDS:K10G6.3i K10G6.3i 5472   II: 3950377-3950396
CDS:K10G6.3j K10G6.3j 4755   II: 3962223-3962344
CDS:K10G6.3b K10G6.3b 5166   II: 3962223-3962344
CDS:K10G6.3c K10G6.3c 5190   II: 3962223-3962344
CDS:K10G6.3o K10G6.3o 4944   II: 3962522-3962853
CDS:K10G6.3d K10G6.3d 4464   II: 3964340-3965280
CDS:K10G6.3m K10G6.3m 4029   II: 3964340-3965280
CDS:K10G6.3s K10G6.3s 3306   II: 3966495-3969217
CDS:K10G6.3a K10G6.3a 5619   II: 3962223-3962344
CDS:K10G6.3e K10G6.3e 2877   II: 3966495-3969217
CDS:K10G6.3g K10G6.3g 5907   II: 3950377-3950396
CDS:K10G6.3k K10G6.3k 4950   II: 3962522-3962853
CDS:K10G6.3l K10G6.3l 4515   II: 3962522-3962853
CDS:K10G6.3n K10G6.3n 5379   II: 3962522-3962853
CDS:K10G6.3p K10G6.3p 351   II: 3970098-3970120
CDS:K10G6.3q K10G6.3q 4458   II: 3964340-3965280
CDS:K10G6.3r K10G6.3r 4893   II: 3964340-3965280

10 RNAi Result

WormBase ID
WBRNAi00110427
WBRNAi00001644
WBRNAi00050561
WBRNAi00016994
WBRNAi00018933
WBRNAi00025945
WBRNAi00025946
WBRNAi00027414
WBRNAi00110429
WBRNAi00110428

661 Allele

Public Name
gk963801
gk963053
h11465
h14347
cxTi9009
otn11915
WBVar01695478
WBVar01695477
WBVar01695479
WBVar01695481
WBVar01695480
WBVar01695483
WBVar01695482
WBVar01437262
WBVar01437260
WBVar01719438
WBVar02029127
WBVar02029126
gk953592
gk953593
WBVar01238355
WBVar01238353
WBVar01369433
WBVar01246551
WBVar01238359
WBVar01238357
WBVar01238358
WBVar01238364
gk139608
gk139607

1 Chromosome

WormBase ID Organism Length (nt)
II Caenorhabditis elegans 15279421  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00004751 3950377 3972140 1

4 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  
Panther orthologue and paralogue predictions  

1 Downstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrII_3972141..3972261   121 II: 3972141-3972261 Caenorhabditis elegans

150 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts that showed significantly increased expression in L1 neural cells comparing to in adult neural cells. DESeq2 (v1.18.1) fold change > 2, P-adj<0.05, using BenjaminiHochberg correction. WBPaper00060811:L1_vs_adult_upregulated_neural
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
adult vs dauer larva Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C. N.A. WBPaper00050488:adult_vs_dauer_regulated_N2_20C
  mRNAs that showed decreased expression in 1 cell mebryo comparing to in oocyte, according to RNAseq analysis. Gaussian error propagation. As cutoff for the up-regulated genes authors used log2 fold change > 1 and P < 0.05 and as cutoff for the down-regulated genes authors used log2 fold change < -1 and P < 0.05. WBPaper00045420:fertilization_downregulated_transcript
Osmotic stress Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when food was present DESeq(version 1.10.1), FDR < 0.05. WBPaper00050726:OsmoticStress_regulated_Food
  Neuronally enriched transcripts according to a comparison of neuronal nuclei IP samples to total nuclei using isolation of nuclei from tagged specific cell types (INTACT) technology. DESEQ2, fold change > 2 and FDR < 0.01. WBPaper00062103:neuron_enriched
  Genes that showed increased expression in wdr-5(ok1417) comparing with in N2. Statistical analysis for misexpression was performed using a moderated t test from the package limma. All genes with a false discovery rate (FDR) of <= 5% (p <= 0.05) were selected as differentially regulated. WBPaper00045861:wdr-5(ok1417)_upregulated
Bacteria infection: Enterococcus faecalis Genes with increased expression after 24 hours of infection by E.faecalis Fold changes shown are pathogen vs OP50. For RNA-seq and tiling arrays, log2 fold changes between gene expression values of infected versus uninfected nematodes were calculated. For log2 fold changes > 0.00001 the values > 81.25th percentile were defined as up-regulated and for log2 fold changes < -0.00001 the values < 18.75th percentile were defined as down-regulated. WBPaper00038438:E.faecalis_24hr_upregulated_TilingArray
  Coexpression clique No. 60, 176662_at-Y53F4B.16, on the genome-wide coexpression clique map for the nematode GPL200 platform. All available microarray datasets for the GPL200 platform (Affymetrix C. elegans Genome Array) were obtained from the GEO repository. This included 2243 individual microarray experiments. These were normalized against each other with the software RMAexpress (Bolstad, 2014). Based on these normalized values, Pearsons correlation coefficients were obtained for each probe-probe pair of the 22,620 probes represented on this array type. The resulting list of correlation coefficients was then ranked to generate the ranked coexpression database with information on each probe represented on the GPL200 platform. WBPaper00061527:176662_at-Y53F4B.16
  Transcripts expressed in the epithelial tissues surrounding the pharynx that includes the arcade and intestinal valve (AIV) cells, according to PAT-Seq analysis using Pbath-15-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:arcade_intestinal-valve_expressed
  Transcripts expressed in body muscle, according to PAT-Seq analysis using Pmyo-3-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:body-muscle_expressed
  Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:GABAergic-neuron_expressed
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:intestine_expressed
  Transcripts expressed in NMDA neuron, according to PAT-Seq analysis using Pnmr-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:NMDA-neuron_expressed
  Transcripts expressed in pharynx, according to PAT-Seq analysis using Pmyo-2-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:pharynx_expressed
  Genes with expression level regulated by genotype (N2 vs CB4856) and age at L3 larva and Late reproduction stage (96 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_age_regulated_developing
  Genes with expression level regulated by genotype (N2 vs CB4856) at old adults stage (214 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_regulated_aging
  Genes with expression level regulated by genotype (N2 vs CB4856) at L3 larva and Late reproduction stage (96 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_regulated_developing
  Transcripts that showed significantly increased expression in day 1 adult hermaphrodite comparing to in L4 larva glp-1(e2141) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-1-adult_vs_L4_upregulated_glp-1(e2141)
  Genes with expression level regulated by genotype (N2 vs CB4856) at Late reproduction stage (96 hours at 24 centigrade). Authors permuted transcript values and used a genome-wide threshold of log10 P-value = 2, which resembles a false discovery rate (FDR) of 0.0118. WBPaper00040858:eQTL_regulated_reproductive
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_12h
  Transcripts that showed significantly increased expression in hrde-1(tm1200) animals, comparing to in N2, after growing at 25C for five generations (late generation). CuffDiff2 WBPaper00051265:F4_hrde-1(tm1200)_upregulated
  Transcripts that showed significantly increased expression in aak-1(tm1944);aak-2(ok524) animals comparing to in N2. DEseq 1.18.0, adjusted p-value < 0.05. WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated
Bacteria infection: Staphylococcus aureus MW2. 4 hours of exposure. Transcripts that showed significantly increased expression after N2 animals had 4 hours of infection by Staphylococcus aureus (MW2). DEseq 1.18.0, adjusted p-value < 0.05. WBPaper00056471:S.aureus-4h_upregulated_N2
  Top 300 transcripts enriched in ABalppppppa, ABpraaapppa according to single cell RNAseq. Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm. WBPaper00061340:ASE_parent
  Transcripts that showed significantly decreased expression in N2 animals exposed to 0.1mM Paraquat from hatching to reaching adult stage. DESeq2 version 1.22.2, p < 0.05 WBPaper00064716:paraquat_downregulated
  Transcripts depleted in purified oocyte P bodies comparing to in the whole animal. DESeq2, FDR < 0.05, fold change > 2. WBPaper00065975:P-body_vs_WholeAnimal_depleted
  Transcripts that showed significantly increased expression after exposure to 75uM paraquat(PQ) from L1 to day 2 adult stage in skn-1(lax188) animals fold change > 2 WBPaper00058711:paraquat_upregulated
  Transcripts detected in body muscle nuclei according to a nuclear FACS-based strategy. Cufflinks WBPaper00065120:body-muscle-transcriptome

11 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr4706 No expression.  
    Expr4705 Expressed in pharyngeal muscle and neuron.  
    Expr1032348 Tiling arrays expression graphs  
    Expr9277 sea-2 is strongly expressed in various tissues, including seam cells, intestine cells, pharyngeal muscles and nerve ring neurons. SEA-2::GFP expression persisted into adulthood. SEA-2::GFP is diffusely localized in both cytoplasm and nucleus.
    Expr1200048 Data from the TransgeneOme project  
    Expr1154264 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr2015704 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr1157224 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr1013282 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  
    Expr2033936 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr1016131 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  

24 GO Annotation

Annotation Extension Qualifier
  involved_in
  involved_in
  involved_in
  involved_in
  enables
  enables
  enables
  enables
  located_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  part_of
  located_in
  located_in
  located_in
  located_in
  located_in
  enables
  enables

1 Homologues

Type
least diverged orthologue

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00004751 3950377 3972140 1

24 Ontology Annotations

Annotation Extension Qualifier
  involved_in
  involved_in
  involved_in
  involved_in
  enables
  enables
  enables
  enables
  located_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  part_of
  located_in
  located_in
  located_in
  located_in
  located_in
  enables
  enables

0 Regulates Expr Cluster

1 Sequence

Length
21764

1 Sequence Ontology Term

Identifier Name Description
gene  

1 Strains

WormBase ID
WBStrain00008588

1 Upstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrII_3941078..3950376   9299 II: 3941078-3950376 Caenorhabditis elegans