WormMine: Gene WBGene00006747

• WormBase Gene ID: WBGene00006747
• Gene Name: unc-7
• Sequence Name: R07D5.1
• Brief Description: unc-7 encodes an innexin required for gap junction formation in invertebrates; UNC-7 is also required for normal locomotion, egg-laying, inhibition of feeding by tapping, avermectin sensitivity, and ivermectin sensitivity, as well as for the antagonism of UNC-79 and UNC-80 activity by volatile anesthetics; unc-7 is genetically required, and transcribed in, neurons rather than muscle cells, from larval stages L1 through L4; homologs of UNC-7 include Drosophila OGRE and SHAKING-B, as well as 24 C. elegans paralogs (including EAT-5, UNC-9, and INX-1 through INX-20); UNC-7 genetically interacts with UNC-124, and unc-7 mutants are phenotypically similar to unc-9 and unc-124 mutants; UNC-7 genetically interacts with AVR-14 and GLC-1 in the response to ivermectin.
• Organism: Caenorhabditis elegans
• Automated Description: Enables gap junction channel activity. Involved in several processes, including regulation of multicellular organismal process; reproductive behavior; and response to anesthetic. Located in gap junction and neuron projection membrane. Expressed in several structures, including OL socket cell; intestine; muscle cell; neurons; and pharyngeal gland cell.
• Biotype: SO:0001217
• Genetic Position: X :22.0336 ±0.049576
• Length (nt): 26895

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00006747

Genomics

11 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:R07D5.1i.1	R07D5.1i.1	2792	X: 15120846-15132200
Transcript:R07D5.1a.2	R07D5.1a.2	2705	X: 15120862-15127750
Transcript:R07D5.1e.1	R07D5.1e.1	2595	X: 15120862-15142346
Transcript:R07D5.1a.1	R07D5.1a.1	2606	X: 15120862-15147740
Transcript:R07D5.1g.1	R07D5.1g.1	2415	X: 15120871-15134673
Transcript:R07D5.1c.1	R07D5.1c.1	2627	X: 15120885-15142395
Transcript:R07D5.1b.1	R07D5.1b.1	1365	X: 15121822-15125448
Transcript:R07D5.1j.1	R07D5.1j.1	1563	X: 15121822-15127574
Transcript:R07D5.1h.1	R07D5.1h.1	1431	X: 15121822-15131809
Transcript:R07D5.1f.1	R07D5.1f.1	1392	X: 15121822-15134595
Transcript:R07D5.1d.1	R07D5.1d.1	1641	X: 15121822-15142346

Other

10 CDSs

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:R07D5.1a	R07D5.1a	1569	X: 15121822-15121970
CDS:R07D5.1e	R07D5.1e	1635	X: 15121822-15121970
CDS:R07D5.1g	R07D5.1g	1386	X: 15121822-15121970
CDS:R07D5.1j	R07D5.1j	1563	X: 15121822-15121970
CDS:R07D5.1f	R07D5.1f	1392	X: 15121822-15121970
CDS:R07D5.1b	R07D5.1b	1365	X: 15121822-15121970
CDS:R07D5.1c	R07D5.1c	1209	X: 15121822-15121970
CDS:R07D5.1d	R07D5.1d	1641	X: 15121822-15121970
CDS:R07D5.1h	R07D5.1h	1431	X: 15121822-15121970
CDS:R07D5.1i	R07D5.1i	1425	X: 15121822-15121970

14 RNAi Result

• WBRNAi00089795
• WBRNAi00040357
• WBRNAi00051462
• WBRNAi00101832
• WBRNAi00010626
• WBRNAi00017543
• WBRNAi00030277
• WBRNAi00089963
• WBRNAi00090123
• WBRNAi00090281
• WBRNAi00090391
• WBRNAi00090438
• WBRNAi00090485
• WBRNAi00090532

496 Allele

• gk964260
• gk964029
• gk962707
• gk964028
• gk963810
• gk963581
• WBVar01928791
• WBVar01928792
• WBVar01928793
• WBVar01928794
• WBVar01928795
• WBVar01928796
• WBVar01928797
• WBVar01928798
• WBVar01928799
• WBVar01928800
• WBVar01928801
• WBVar01928802
• WBVar01928803
• WBVar01928804
• WBVar01928805
• WBVar01928806
• WBVar01928807
• WBVar01928808
• WBVar01928809
• WBVar01928810
• WBVar01928811
• gk963156
• gk916074
• gk302713

1 Chromosome

• WormBase ID: X
• Organism: Caenorhabditis elegans
• Length (nt): 17718942

1 Chromosome Location

• Feature . Primary Identifier: WBGene00006747
• Start: 15120846
• End: 15147740
• Strand: -1

4 Data Sets

• WormBaseAcedbConverter
• GO Annotation data set
• C. elegans genomic annotations (GFF3 Gene)
• Panther orthologue and paralogue predictions

1 Downstream Intergenic Region

• WormBase ID: intergenic_region_chrX_15117159..15120845
• Length (nt): 3687
• Chromosome Location: X: 15117159-15120845
• Organism: Caenorhabditis elegans

148 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts that showed significantly increased expression in L1 neural cells comparing to in adult neural cells.	DESeq2 (v1.18.1) fold change > 2, P-adj<0.05, using BenjaminiHochberg correction.	WBPaper00060811:L1_vs_adult_upregulated_neural
	Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons.	DESeq. False discovry rate (FDR) < 0.1.	WBPaper00048988:neuron_expressed
adult vs dauer larva	Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C.	N.A.	WBPaper00050488:adult_vs_dauer_regulated_N2_20C
Osmotic stress	Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when food was present	DESeq(version 1.10.1), FDR < 0.05.	WBPaper00050726:OsmoticStress_regulated_Food
	Neuronally enriched transcripts according to a comparison of neuronal nuclei IP samples to total nuclei using isolation of nuclei from tagged specific cell types (INTACT) technology.	DESEQ2, fold change > 2 and FDR < 0.01.	WBPaper00062103:neuron_enriched
Bacteria infection: Enterococcus faecalis	Genes with increased expression after 24 hours of infection by E.faecalis Fold changes shown are pathogen vs OP50.	For RNA-seq and tiling arrays, log2 fold changes between gene expression values of infected versus uninfected nematodes were calculated. For log2 fold changes > 0.00001 the values > 81.25th percentile were defined as up-regulated and for log2 fold changes < -0.00001 the values < 18.75th percentile were defined as down-regulated.	WBPaper00038438:E.faecalis_24hr_upregulated_TilingArray
	Coexpression clique No. 203, sre-33-ZK1025.1_8337, on the genome-wide coexpression clique map for the nematode GPL200 platform.	All available microarray datasets for the GPL200 platform (Affymetrix C. elegans Genome Array) were obtained from the GEO repository. This included 2243 individual microarray experiments. These were normalized against each other with the software RMAexpress (Bolstad, 2014). Based on these normalized values, Pearsons correlation coefficients were obtained for each probe-probe pair of the 22,620 probes represented on this array type. The resulting list of correlation coefficients was then ranked to generate the ranked coexpression database with information on each probe represented on the GPL200 platform.	WBPaper00061527:sre-33-ZK1025.1_8337
	Coexpression clique No. 60, 176662_at-Y53F4B.16, on the genome-wide coexpression clique map for the nematode GPL200 platform.	All available microarray datasets for the GPL200 platform (Affymetrix C. elegans Genome Array) were obtained from the GEO repository. This included 2243 individual microarray experiments. These were normalized against each other with the software RMAexpress (Bolstad, 2014). Based on these normalized values, Pearsons correlation coefficients were obtained for each probe-probe pair of the 22,620 probes represented on this array type. The resulting list of correlation coefficients was then ranked to generate the ranked coexpression database with information on each probe represented on the GPL200 platform.	WBPaper00061527:176662_at-Y53F4B.16
	Transcripts expressed in the epithelial tissues surrounding the pharynx that includes the arcade and intestinal valve (AIV) cells, according to PAT-Seq analysis using Pbath-15-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:arcade_intestinal-valve_expressed
	Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:GABAergic-neuron_expressed
	Transcripts that showed significantly decreased expression in atfs-1(cmh15) (null allele) animals comparing to in N2 animals at L4 larva stage.	edgeR, fold change > 2, FDR < 0.05	WBPaper00060909:atfs-1(cmh15)_downregulated
	Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:intestine_expressed
	Transcripts expressed in NMDA neuron, according to PAT-Seq analysis using Pnmr-1-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:NMDA-neuron_expressed
	Transcripts that showed significantly decreased expression in day 3 adult hermaphrodite comparing to in L4 larva daf-16(mu86);glp-1(e2141) animals.	Fold change > 2, FDR < 0.05	WBPaper00064088:Day-3-adult_vs_L4_downregulated_daf-16(mu86);glp-1(e2141)
	Transcripts that showed significantly decreased expression in day 3 adult hermaphrodite comparing to in L4 larva glp-1(e2141) animals.	Fold change > 2, FDR < 0.05	WBPaper00064088:Day-3-adult_vs_L4_downregulated_glp-1(e2141)
	Transcripts that showed significantly increased expression after four-day-old young adult worms were placed on NGM plates seeded with OP50 in the presence 5% Agaro-oligosaccharides(AGO) for 24 h, comparing to animals grown in the absence of AGO.	Fold change > 2.	WBPaper00064306:Agaro-oligosaccharides_upregulated
	Transcripts that showed significantly increased expression in sin-3(tm1276) comparing to in N2.	DESeq2, fold change > 2, p-value < 0.01.	WBPaper00061203:sin-3(tm1276)_upregulated
	Transcripts that showed significantly increased expression in aak-1(tm1944);aak-2(ok524) animals comparing to in N2.	DEseq 1.18.0, adjusted p-value < 0.05.	WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated
	Transcripts that showed significantly increased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_upregulated
	Transcripts that showed significantly decreased expression in N2 animals exposed to 0.1mM Paraquat from hatching to reaching adult stage.	DESeq2 version 1.22.2, p < 0.05	WBPaper00064716:paraquat_downregulated
	Transcripts that showed significantly altered expression after 24 hour exposure to stavudine (d4T) starting at L1 lava stage.	DESeq	WBPaper00053302:stavudine_24h_regulated
	Transcripts that showed significantly decreased expression in sin-3(tm1276) comparing to in N2 at early embryo when there were only 3 -5 eggs in the adult.	DESeq2, fold change > 2, adjusted p-value < 0.01	WBPaper00058598:sin-3(tm1276)_downregulated
	Transcripts that showed significantly increased expression in sftb-1(cer6) deletion homozygous comparing to to in N2 animals at L4 larva stage.	DESeq2, fold change > 2	WBPaper00058725:sftb-1(cer6)_downregulated
	Transcripts that showed significantly increased expression in hpk-1(pk1393) comparing to in N2 at adult day 2.	DESeq 2, fold change > 2, FDR < 0.05.	WBPaper00065581:hpk-1(pk1393)_upregulated
	Transcripts that showed significantly increased expression in hda-1(ne4752[3xFLAG-Degron-HDA-1]) in gonads dissected from 1-day old adult animals.	Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R.	WBPaper00061479:hda-1(ne4752)_upregulated
	Transcripts that showed significantly increased expression in wdr-23(mac32) embryos from parents fed with E. coliHB101, comparing to N2 embryos parents fed with E. coli HB101.	DESeq2, Fold Change > 2 or < 0.5.	WBPaper00059566:wdr-23(mac32)_upregulated
	Transcripts that showed significantly increased expression in hda-2(ok1479) comparing to in N2 animals.	DESeq2 (version 1.28.1), FDR < 0.01, fold change > 2.	WBPaper00062159:hda-2(ok1479)_upregulated
	Transcripts that showed significantly increased expression in npr-15(tm12539) comparing to in N2 at L4 larva stage.	Fold change > 2, FDR < 0.05.	WBPaper00066608:npr-15(tm12539)_upregulated
	Transcripts that showed significantly increased expression in srbc-48(ac23);kyIs262;fer-1(b232ts) comparing to in kyIs262;fer-1(b232ts), 24h after infection with P.aeruginosa.	DESeq2, FDR <0.05, fold change > 2.	WBPaper00059664:srbc-48(ac23)_upregulated
	Transcripts that showed significantly increased expression in dpy-21(e428) comparing to in N2 during L3 stage.	DESeq v1.6.3. Fold change > 1.5.	WBPaper00050370:dpy-21(e428)_L3_upregulated

20 Expression Patterns

• Primary Identifier: Expr8588
• Remark: Picture: Figure 4, Figure S2A.
• Pattern: Whole-mount immunofluorescent staining of wild-type animals with an antibody against UNC-7 revealed specific staining in both the nervous system and muscle. Along the processes of dorsal and ventral nerve cords, UNC-7 localizes to many small discrete puncta interspersed by infrequent larger puncta. Antibody staining against UNC-7 in a strain expressing a postsynaptic GABA receptor marker, UNC-49::GFP (oxIs22), further confirmed that UNC-7 puncta is present along nerve processes apposed to muscle. UNC-7 also localizes to discrete puncta outlining the surface of body-wall muscles, mostly at posterior muscles, and along muscle arms that extend perpendicular from toward the nerve cords.
• Subcellular Localization: Double immunolabeling with various presynaptic markers. Endogenous UNC-7 did not colocalize with active zone proteins UNC-10 and SYD-2. No colocalization were observed between UNC-7 and presynaptic GFP markers, GFP::SYD-2 or SNB-1::GFP, expressed in GABAergic neurons. Instead, many UNC-7 puncta are perisynaptic, closely flanking presynaptic markers. Also, UNC-7 puncta were not directly apposed to postsynaptic UNC-49::GFP puncta but appeared perisynaptic, further suggesting that the many small UNC-7 puncta flank presynaptic regions.

• Primary Identifier: Expr8692
• Remark: Picture: N.A.
• Pattern: Expression in the alimentary canal: Weak or rare expression in posterior arcades, g1, g2. Expression in the nervous system: AIN, AVA, AVB, AVD, AVG, AVK, DDn (3-fold to early larva), DVA, DVB, DVC, IL1, OLL, PVD, PVQ, PVT, RIV, RME, RMG, SDQ, SIA, SIB, SMB, VCn. Expression in the reproductive system: In adult stage, expressed in VCn. In developing larva stage, expressed in VCn.

• Primary Identifier: Expr15219
• Pattern: In transgenic worms, unc-7b GFP expression was observed in many neurons in the head including the two AVA interneurons, suggesting that UNC-7b is expressed in AVA.

• Primary Identifier: Expr8693
• Remark: Picture: N.A.
• Pattern: Expression in the alimentary canal: Weak or rare expression in intestine. Expression in the nervous system: OLso, AVD, AVG, AVJ, AVK, DVA, DVC, IL1, PVP, RIC, RIF, RMD, SIB, SMD, URA.

• Primary Identifier: Expr15711
• Pattern: mCherry-tagged MEC-4 protein was distributed in a punctate pattern along the ALM and PLM dendrites. GFP-tagged UNC-7 was also expressed in a punctate pattern in both touch receptor neuron types. However, little overlap was observed between UNC-7 and MEC-4 puncta in either cell type. Thus, UNC-7 does not appear to physically associate with MEC-4-containing mechanotransduction complexes, consistent with a distinct functional role in touch sensation.

• Primary Identifier: Expr8716
• Remark: Picture: Fig 4A.
• Pattern: In wild-type animals, UNC-7 (representing all isoforms) is expressed throughout the nervous system, as well as in body muscles, particularly in the posterior.
• Subcellular Localization: Total UNC-7 is often localized to puncta that may resent gap junctions.

• Primary Identifier: Expr8717
• Remark: Picture: Figure 4C. UNC-7::GFP expression mirrored that observed with anti-UNC-7 antibodies. However, some cell bodies could now be recognized with variability, probably due to over-expression from the rescuing array. Because in these animals UNC-7L::GFP expression was not detected by western blot analysis, authors attribute the majority of this signal to UNC-7S::GFP and conclude that UNC-7S is broadly expressed in many interneurons and motor neurons.
• Pattern: The strongest signals appeared late in embryogenesis and in L1 animals, coinciding with unc-7 mRNA levels, and UNC-7::GFP was detected in all motor neuron classes in the vental nerve cord; in older larvae expression was seen in all post-embryonically derived motor neuron classes (VA, VB, AS, VD, and VC).

• Primary Identifier: Expr2036045
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr1032821
• Pattern: Tiling arrays expression graphs

• Primary Identifier: Expr12312
• Pattern: UNC-7 expression was detected in sex muscles (anal depressor, gubernacular erector, gubernacular retractor, and anterior oblique).

• Primary Identifier: Expr12313
• Pattern: In these transgenic males, UNC-7 expression was found, in addition to the sex muscles (Expr12312), in several mating circuit neurons (SPC, PCA, PCB, HOA, and unidentified ray neurons). Similar to previous studies (Starich et al., 2009; Yeh et al., 2009; Kawano et al., 2011), this reporter also expresses in head ganglion neurons, several ventral chord neurons, and tail neurons of the hermaphrodite, in addition to few unreported pharyngeal neurons (I5, M2, I4, M3, and I1).

• Primary Identifier: Expr12407
• Pattern: Expression was detected in sex muscles (anal depressor, gubernacular erector, gubernacular retractor, and anterior oblique).

• Primary Identifier: Expr12408
• Pattern: With a construct including 8 kb upstream of the first exon and the entire unc-7 genomic region fused to YFP, UNC-7 expression was found, in addition to the sex muscles reported in Expr12407, in several mating circuit neurons (SPC, PCA, PCB, HOA, HOB, and unidentified ray neurons). Similar to previous studies (Starich et al., 2009; Yeh et al., 2009; Kawano et al., 2011), this reporter also expresses, in head ganglion neurons, several ventral chord neurons, and tail neurons of the hermaphrodite, in addition to few unreported pharyngeal neurons (I5, M2, I4, M3, and I1).

• Primary Identifier: Expr1942
• Remark: Reporter gene fusion type not specified.
• Pattern: Many neurons in head, tail and vnc.

• Primary Identifier: Expr1155149
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015

• Primary Identifier: Expr1026014
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012

• Primary Identifier: Expr1144423
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015

• Primary Identifier: Expr2017909
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr1015441
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012

• Primary Identifier: Expr1507
• Pattern: The 2.7 transcript is present in all stages but most abundant in L1, L2 and L3, and greatly decreased by adult stage.

35 GO Annotation

8 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00006747
• Start: 15120846
• End: 15147740
• Strand: -1

35 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 26895

1 Sequence Ontology Term

• Name: gene

12 Strains

• WBStrain00034228
• WBStrain00034123
• WBStrain00034360
• WBStrain00037230
• WBStrain00040856
• WBStrain00054742
• WBStrain00008429
• WBStrain00004110
• WBStrain00008423
• WBStrain00004076
• WBStrain00008419
• WBStrain00008428

1 Upstream Intergenic Region

• WormBase ID: intergenic_region_chrX_15147741..15151115
• Length (nt): 3375
• Chromosome Location: X: 15147741-15151115
• Organism: Caenorhabditis elegans