WormMine: ExpressionPattern Expr11963

• Pattern: The anti-PAT-6/actopaxin antibody stains body wall muscle cell attachments of wild-type and pat-6 embryos that have been rescued by a functional pat-6::Green Fluorescent Protein (GFP) transgene.
• Primary Identifier: Expr11963
• Subcellular Localization: In adult hermaphrodites, PAT-6::GFP is also located in the attachments of other muscles, including the uterine, anal depressor, anal sphincter, and vulval muscles, as well as in the spermatheca and the distal tip cells. In wild-type 500 min embryos, PAT-6/actopaxin is polarized and located in nascent attachment complexes within the basal sarcolemma. PAT-6/actopaxin is first detectable in the myoblasts of early embryos, where it is distributed throughout the cytoplasm. When the embryos reach the 1.5-fold stage of development (430 min postfertilization), most of the PAT-6/actopaxin is polarized within the muscle cells and has entered nascent attachments. In each body wall muscle quadrant,PAT-6/actopaxin appears as a single longitudinal stripe of tightly clustered foci. These attachments have not yet moved into a regular striated array, and distinguishable dense body and M lines are not yet visible. PAT-6::GFP continues to colocalize with integrin at the body wall muscle attachments during the remaining stages of embryogenesis, larval development, and adulthood. PAT-6::GFP is found in adult dense bodies, M lines, and muscle-muscle adhesion plaques. Functional PAT-6/actopaxin::Cyan Fluorescent Protein (CFP) and PAT-3/integrin::Yellow Fluorescent Protein (YFP) fusion proteins expressed from a transgenic extrachomosomal array colocalize extensively in adult muscle attachments.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
somatic cell that situates at the tip of a gonad arm.	distal tip cell	DTC	WBbt:0006865
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292
a saddle-shaped muscle cell that encircles the intestinal-rectal valve	anal sphincter muscle	lineage name: ABprpppppap	WBbt:0005798
muscle lining of the uterine wall.	uterine muscle		WBbt:0005342

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003932	pat-6	T21D12.4	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023