WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  lin-12(1in_1408) displayed a highly differentiated, characteristic expression pattern in the VPC lineages. The expression was already evident in comma-stage embryos. Several glowing cells of different types were seen along the anteroposterior body axis at the twofold embryonic stage. At the L1 stage, lin-12(1in_1408) was expressed in all Pn.p cells. Some unidentified cells in the head and tail regions were also gfp-positive at this stage. By the early L2 stage, lin-12(1in_1408) expression remained strong only in the P(3-8).p cells. Unlike the other Pn.p cells, they remain unfused with the hypodermal syncytium, thereby retaining their competence to differentiate into a vulval cell type. After vulval induction at the early L3 stage, lin-12(1in_1408) was strongly expressed in P5.p and P7.p, the two VPCs that adopt 2nd vulval fates, but weakly detectable in the other VPCs, P(3-4,6,8).p. As shown earlier (Wilkinson and Greenwald, 1995; 276 Levitan and Greenwald, 1998), lin-12 expression remained on in the P5.p and P7.p granddaughters by the late L3 stage. In addition to the P5.p and P7.p descendants, we found obvious fluorescence in the daughters of other VPCs, and even in the P6.p granddaughters [the daughters of P(3,4,8).p fuse with the hypodermis]. Nevertheless, expression of lin-12(1in_1408) was constantly intense in the P5.p and P7.p lineages, as compared with other VPC lineages. At the L2 and early L3 stages, lin-12(1in_1408) is also expressed in the two AC/VU precursors. Later, when lin-12(1in_1408) expression became increased in P5.p and P7.p relative to the other VPCs, transgene activity was seen in the presumptive VU cell, but not in the AC. During the L4 stage, expression of lin-12(1in_1408) was gradually lost from the P6.p lineage; only the P(5,7).p descendants retained GFP fluorescence in the developing vulval tissue. It is worth to note that lin-12(1in_1408) was also active in certain hypodermal and intestinal cells throughout larval development, presumably due to background expression of the vector. In adult hermaphrodites, lin-12(1in_1408) expression could not be detected. The only exception was presented by a few unidentified cells in the posterior body part, probably due to background expression. Primary Identifier  Expr10737

18 Anatomy Terms

Definition Name Synonym Primary Identifier
posterior region, from rectum to the end tail   WBbt:0005741
Posterior descendent cell of P2 fuses into postembryonic large hypodermal syncytium hyp7. P2.p   WBbt:0006890
Posterior descendent cell of P1 fuses into postembryonic large hypodermal syncytium hyp7. P1.p   WBbt:0006889
descendent cell of P12, posterior P12.p   WBbt:0006900
descendent cell of P11, posterior P11.p   WBbt:0006899
descendent cell of P10, posterior P10.p   WBbt:0006898
Posterior descendent cell of P9 fuses into postembryonic large hypodermal syncytium hyp7. P9.p   WBbt:0006897
  P6.p hermaphrodite   WBbt:0008125
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite. P5.p hermaphrodite   WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite. P4.p hermaphrodite   WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite. P3.p hermaphrodite   WBbt:0008112
  P8.p hermaphrodite   WBbt:0008133
  P7.p hermaphrodite   WBbt:0008129
anterior-most body region containing the pharynx. head   WBbt:0005739
Ventral Uterine precursor cell. ventral uterine precursor VU WBbt:0006780
post-embryonic cell of pedigree Z4.aaa Z4.aaa   WBbt:0007688
post-embryonic cell of pedigree Z1.ppp Z1.ppp   WBbt:0007667
Blast cell lying ventrolaterally in the late embryo and L1 larval stage which divides during the late L1 larva in stereotypical reiterated fashion to give rise to many motor neurons which begin function in the ventral cord and preanal ganglion of the early L2 larva, as well as hypodermal cells (which fuse into hyp7) and a series of programmed cell deaths. In addition, the Pn.p daughters of P3 through P8 lineages persist without fusing into the hyp7 syncytium until mid L3 stage when they can be induced to divide further to produce vulval epithelial cells (vulA - vulF) or more hyp7 cells. P0 is also known as W. ventral cord blast cell P cell WBbt:0008115

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003001 lin-12 R107.8 Caenorhabditis elegans

8 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo. comma embryo Ce WBls:0000017
  The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo. 2-fold embryo Ce WBls:0000019
  The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage. 3-fold embryo Ce WBls:0000020
  The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo. 1.5-fold embryo Ce WBls:0000018