WormMine: AnatomyTerm WBbt:0003831

• Definition: Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath
• Name: AWAL
• Primary Identifier: WBbt:0003831
• Synonym: lineage name: ABplaapapaa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABplaapapaa	ABplaapapaa nucleus		WBbt:0001708

3 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Top 300 transcripts enriched in ASGL, ASGR, AWAL, AWAR according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:ASG_AWA
	Top 300 transcripts enriched in AWAL, AWAR according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:AWA
	Single-cell RNA-Seq cell group 106_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:106_0

125 Expression Patterns

• Primary Identifier: Expr4575
• Remark: Reporter gene fusion type not specified.
• Pattern: To identify the expression sites of the ABC transporter PGP-2, five independent reporter gene expressing strains carrying a pgp-2::GFP fusion construct were generated and analysed. In all cases, PGP-2-GFP was found in the AWA sensory neuron pair. This conclusion was validated by analysing the morphology of the cilia. The PGP-2 expressing neurons showed the characteristic branched cilium morphology typical of the AWA neurons.

• Primary Identifier: Expr4647
• Pattern: Transgenic lines generated with the partial protein fusion construct (for example, ljEx109) expressed TRPA-1:: GFP in the same cells as ljEx107 (see Expr4646), but with some additional cells, including the majority of amphid sensory neurons (for example, ASH, AWA, AWB, ASI and ASK) and the phasmid neurons PHA and PHB.

• Primary Identifier: Expr4648
• Pattern: Transgenic lines generated with the full-length protein fusion construct (for example, ljEx114) expressed TRPA-1:: GFP in the same cells as ljEx107 (see Expr4646), but with some additional cells, including the majority of amphid sensory neurons (for example, ASH, AWA, AWB, ASI and ASK) and the phasmid neurons PHA and PHB. The full-length TRPA-1:: GFP fusion was also expressed in the PVD and PDE in the postdeirid sensilla and the sensory neurons OLQ and IL1. Other neurons in the head and ventral nerve cord also expressed TRPA-1:: GFP.
• Subcellular Localization: The fusion protein was observed at the cilia of sensory neurons, as well as at the cell body.

• Primary Identifier: Expr4526
• Pattern: Expressed in AWAL/R, ASIL/R, RIAL/R, PVT.

• Primary Identifier: Expr15619

• Primary Identifier: Expr3175
• Pattern: Expressed in ADF, ADL, AFD, ASE, ASG, ASH, ASI, ASJ, ASK, AWA, AWB, AWC, BAG, PHA, PHB, URX, intestine.

• Primary Identifier: Expr8976
• Remark: Picture: Fig 4E to 4H. npr-5 = Y58G8A.4 in this paper.
• Pattern: The npr-5p::RFP transgene was expressed in a subset of amphid neurons (ADF, ASE, ASG, ASI, ASJ, ASK, AWA, AWB) in the inner labial neuron IL2, in the interneurons AIA and AUA, and in the phasmids (PHA, PHB). Outside the nervous system, npr-5p::RFP was expressed in head, neck, and body muscles throughout larval and adult development.

• Primary Identifier: Marker84
• Remark: Picture: Figure 1I and 1M.
• Pattern: Marker for AWA cilia.

• Primary Identifier: Expr11753
• Remark: genomic

• Primary Identifier: Expr11755
• Remark: Isoform 1b.1

• Primary Identifier: Expr11756
• Remark: Isoform 1b.2

• Primary Identifier: Expr15558

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr15606

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr1824
• Remark: Cells expressing TAX-6 were visualized using several tax-6::gfp fusion genes. The expression of tax-6 is under diverse transcriptional controls. Reporter gene fusion type not specified. pAK43 is a larger transgene that should include all promoter regions for tax-6 transcription, since another gene is encoded just upstream of this region and tax-6 mRNA does not seem to be derived from a polycistronic transcript.
• Pattern: When introduced into wildtype animals, pAK43 drove TAX-6 expression in many sensory neurons, as well as interneurons including AIY and AIZ, and most, if not all, muscle cells. pAK43 is expressed in muscle, AIB, AIY, AIZ, RIA, RIB, RIS, RIM, ASI, ADF, ASH, ASK, ADL, AUA, PHA, PHB, AVE. It is also expressed in AFD, ASE, AWA, AWC, AVK, AIM, RMDV, AVA.

• Primary Identifier: Expr2611
• Pattern: Both gfp reporter transgenes showed strong expression in two bilaterally symmetric pairs of neurones in the head of the animal. These neurones was identified as the AWAL/R and AWCL/R chemosensory neurones. In addition to the four chemosensory neurones, the transcriptional fusion was expressed in muscle and hypodermal cells throughout the animal. The expression of SRA-13 in the chemosensory neurones suggested that, like the other SRA proteins, SRA-13 might be involved in sensing the environment.
• Subcellular Localization: In the AWA and AWC neurones, the translational SRA-13::GFP fusion protein was present in the cell body, the axons and the dendrites.

• Primary Identifier: Expr14899
• Pattern: We found the expression of the ins-4 reporter in several neurons, including the sensory neurons AWA, ASI, and URX.

• Primary Identifier: Expr11760
• Remark: Reporter fusion construct not specified.

11 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes.		late cleavage stage embryo Ce	WBls:0000014

5 Parents

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
embryonic cell	ABplaapapa		WBbt:0006120
Neuron class of two ciliated neurons that are associated with the sheath cells of the amphid sensilla.	AWA		WBbt:0005670
neuron type, cell body located in left lateral ganglion.	lateral ganglion left neuron		WBbt:0005102
	amphid left sensillum		WBbt:0005395