WormMine: ExpressionPattern Expr3695

• Pattern: Fluorescence was observed in epithelial cells that synthesize cuticle. Expressed in the hypodermis, including the major body syncytium, hyp7, and hypodermal cells in the head and tail. A pulse of fluorescence was observed in the hypodermis prior to molting. Fluorescence from mlt-8p::gfp-pest was first detected approximately 3 h before the L1/L2 molt, or 13 h after hatchlings synchronized by starvation were fed and incubated at 25 centigrades. The intensity of fluorescence increased until lethargus and then decreased rapidly, such that GFP was barely detectable just 2 h after molting. When monitoring individual transgenic larvae over the course of development, fluorescence from mlt-8p::gfp-pest was observed from 65 +/- 2% to 90 +/- 2% of the duration of each larval stage. The mlt-8 reporter was expressed, in larvae, in a single posterior neuron that remains to be identified. Expression of the gfp fusion genes was never detected in the hypodermis of gravid adults that no longer molt.
• Primary Identifier: Expr3695

12 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
Cylindrical hypodermal syncytium in head	hyp3	lineage name: ABplaapaaaa	WBbt:0004690
Cylindrical hypodermal syncytium in head	hyp4	lineage name: ABarpapapa	WBbt:0004687
Cylindrical hypodermal syncytium in head	hyp1	lineage name: ABalpaapppa	WBbt:0004694
Tail ventral hypodermis	hyp10	lineage name: ABplppppppp	WBbt:0004378
Tail ventral hypodermis, 8th hypodermis cell	hyp8		WBbt:0004381
Tail ventral hypodermis, 9th hypodermis cell	hyp9		WBbt:0004380
Cylindrical hypodermal syncytium in head	hyp2	lineage name: ABalpaapppp	WBbt:0004692
Tail dorsal hypodermis	hyp11	lineage name: Cpappv	WBbt:0004377
Cylindrical hypodermal syncytium in head	hyp5	lineage name: ABarpappap	WBbt:0004685
Preanal hypodermis	hyp12	lineage name: P12.pa	WBbt:0004376
Cylindrical hypodermal syncytium in head	hyp6	lineage name: ABarpaapaa	WBbt:0004679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00021095	mlt-8	W08F4.6	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003