WormMine: ExpressionPattern Expr2280

• Pattern: The R16 antibody stained muscle cells of wild-type from the comma stage to the 3-fold stage, but not those of x14 animals. The R16 antibody staining appeared at the later comma stage and became strong at the 1.5-fold stage, when the muscle starts twitching, and continued to the adult stage. Although background staining from the early embryo to pre-comma stages was also observed in the cytoplasm of whole cells, this staining was not different between wild-type and x14 animals. The R16 antibody stained the body wall, pharyngeal, vulval and the anal muscles of adult hermaphrodites and male tail muscles in wild-type, but not in unc-68(x14) animals. The identity of these muscle tissues was confirmed by double-staining with rhodaminephalloidin. Among the anal muscles, the R16 antibody stained the depressor muscle but did not stain the sphincter muscle. The R16 antibody also stained pharyngeal muscles, especially those in the terminal bulb and isthmus. The staining pattern in body wall muscle was observed to be of a series of small dots. Because the antiserum stained in the intestines of both wild-type and unc-68(x14) animals, the intestinal staining is likely to represent the cross-reaction of the R16 antibody with unknown proteins.
• Primary Identifier: Expr2280
• Remark: The tissue-specific expression of unc-68 protein suggested by antibody staining was confirmed by a gfp-fusion construct. The results of unc-68::gfp expression and immunostaining experiments strongly suggest that CeRyR is present only in muscles. See Expr2281 for GFP result.

13 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292
	copulatory spicule		WBbt:0005312
	spicule retractor muscle		WBbt:0005827
	spicule protractor muscle		WBbt:0005826
specialized muscle in the male.	diagonal muscle	dgl	WBbt:0005815
specialized muscle type in the posterior of adult male.	longitudinal male muscle	mu_male_long	WBbt:0006909
	gubernacular erector muscle		WBbt:0005824
specialized muscle in the male.	posterior oblique muscle		WBbt:0005823
specialized muscle in the male.	anterior oblique muscle		WBbt:0005822
	gubernacular retractor muscle		WBbt:0005825

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006801	unc-68	K11C4.5	Caenorhabditis elegans

10 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019