WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00003623 Gene Name  nhr-25
Sequence Name  ? F11C1.6 Brief Description  nhr-25 encodes a nuclear hormone receptor orthologous to Drosophila Ftz-F1; NHR-25 is required for embryogenesis, molting, vulval and gonadal development, and hypodermal expression of acn-1; nhr-25 is expressed in gonads and loaded into embryos as a maternal transcript; nhr-25 is zygotically expressed in progeny of the E cell, and then in hypodermis and gut; the role of NHR-25 in molting may be evolutionarily conserved between nematodes and arthopods.
Organism  Caenorhabditis elegans Automated Description  Enables armadillo repeat domain binding activity. Involved in molting cycle and positive regulation of nematode male tail tip morphogenesis. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in nucleus. Expressed in several structures, including excretory system; gonad; hypodermal cell; tail; and vulval precursor cell. Human ortholog(s) of this gene implicated in several diseases, including gonadal dysgenesis (multiple); primary ovarian insufficiency 7; and spermatogenic failure 8. Is an ortholog of human NR5A2 (nuclear receptor subfamily 5 group A member 2).
Biotype  SO:0001217 Genetic Position  X :9.49412 ±0.083721
Length (nt)  ? 5472
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00003623

Genomics

3 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:F11C1.6a.2 F11C1.6a.2 2572   X: 13008493-13013964
Transcript:F11C1.6a.1 F11C1.6a.1 2474   X: 13008886-13013962
Transcript:F11C1.6b.1 F11C1.6b.1 2353   X: 13009266-13013964
 

Other

2 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:F11C1.6a F11C1.6a 1719   X: 13008892-13009008
CDS:F11C1.6b F11C1.6b 1479   X: 13009481-13009499

143 RNAi Result

WormBase ID
WBRNAi00095564
WBRNAi00099066
WBRNAi00038539
WBRNAi00038540
WBRNAi00044365
WBRNAi00008639
WBRNAi00038537
WBRNAi00038538
WBRNAi00025078
WBRNAi00066783
WBRNAi00065941
WBRNAi00115650
WBRNAi00115649
WBRNAi00068493
WBRNAi00068495
WBRNAi00068494
WBRNAi00068497
WBRNAi00068496
WBRNAi00068937
WBRNAi00115647
WBRNAi00091266
WBRNAi00085753
WBRNAi00115646
WBRNAi00084427
WBRNAi00084419
WBRNAi00084421
WBRNAi00084423
WBRNAi00098838
WBRNAi00084424
WBRNAi00084426

95 Allele

Public Name
gk964260
gk964029
gk962707
gk964028
gk963810
gk963581
WBVar01691274
WBVar01691273
WBVar01691275
gk364153
gk864691
gk297666
gk297665
gk297670
gk297669
gk297668
gk297667
gk297674
gk297673
gk297672
gk297671
gk297675
WBVar01602359
WBVar01602361
WBVar01602360
kry6
h17041
WBVar01817152
gk887523
WBVar01471893

1 Chromosome

WormBase ID Organism Length (nt)
X Caenorhabditis elegans 17718942  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00003623 13008493 13013964 1

4 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  
Panther orthologue and paralogue predictions  

1 Downstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrX_13013965..13014449   485 X: 13013965-13014449 Caenorhabditis elegans

217 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts of coding genes that showed significantly decreased expression in muscle. DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed. WBPaper00062325:muscle_depleted_coding-RNA
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
adult vs dauer larva Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C. N.A. WBPaper00050488:adult_vs_dauer_regulated_N2_20C
Osmotic stress Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when food was present DESeq(version 1.10.1), FDR < 0.05. WBPaper00050726:OsmoticStress_regulated_Food
Osmotic stress Transcripts that showed significantly altered expression with 500 mM salt (NaCl) vs 100 mM salt when no food was present DESeq(version 1.10.1), FDR < 0.05. WBPaper00050726:OsmoticStress_regulated_NoFood
Bacteria infection: Enterococcus faecalis Genes with increased expression after 24 hours of infection by E.faecalis Fold changes shown are pathogen vs OP50. For RNA-seq and tiling arrays, log2 fold changes between gene expression values of infected versus uninfected nematodes were calculated. For log2 fold changes > 0.00001 the values > 81.25th percentile were defined as up-regulated and for log2 fold changes < -0.00001 the values < 18.75th percentile were defined as down-regulated. WBPaper00038438:E.faecalis_24hr_upregulated_TilingArray
  Transcripts that showed significantly increased expression after animals were treated with 50uM Rifampicin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rifampicin_upregulated
  Transcripts that showed significantly higher expression in somatic gonad precursor cells (SGP) vs. head mesodermal cells (hmc). DESeq2, fold change >= 2, FDR <= 0.01. WBPaper00056826:SGP_biased
  Transcripts that showed significantly increased expression after animals were treated with 100uM Rapamycin and 50mM Metformin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rapamycin-Metformin_upregulated
  Genes that showed significantly increased expression in wrn-1(gk99) comparing to in N2, according to RNAseq. DESeq was used to calculate the fold changes, log fold changes, and significance of the changes for each comparison. WBPaper00045934:wrn-1(gk99)_upregulated
  Transcripts expressed in body muscle, according to PAT-Seq analysis using Pmyo-3-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:body-muscle_expressed
  Transcripts that showed significantly decreased expression at 5-days-post L4 adult N2 hermaphrodites comparing to 1-day-post L4 adult N2 hermaphrodites. DESeq2, fold change > 2, FDR < 0.05 WBPaper00065835:Day5_vs_Day1_downregulated
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts that showed significantly decreased expression in atfs-1(cmh15) (null allele) animals comparing to in N2 animals at L4 larva stage. edgeR, fold change > 2, FDR < 0.05 WBPaper00060909:atfs-1(cmh15)_downregulated
  Genes with expression level regulated by genotype (N2 vs CB4856) and age at old adults stage (214 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_age_regulated_aging
  Genes with expression level regulated by genotype (N2 vs CB4856) at old adults stage (214 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_regulated_aging
  Transcripts that showed significantly decreased expression in day 3 adult hermaphrodite comparing to in L4 larva daf-16(mu86);glp-1(e2141) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-3-adult_vs_L4_downregulated_daf-16(mu86);glp-1(e2141)
  Transcripts that showed significantly decreased expression in day 3 adult hermaphrodite comparing to in L4 larva glp-1(e2141) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-3-adult_vs_L4_downregulated_glp-1(e2141)
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 10 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.1mix_downregulated_12h
  Transcripts that showed significantly increased expression after four-day-old young adult worms were placed on NGM plates seeded with OP50 in the presence 5% Agaro-oligosaccharides(AGO) for 24 h, comparing to animals grown in the absence of AGO. Fold change > 2. WBPaper00064306:Agaro-oligosaccharides_upregulated
  Transcripts that showed significantly increased expression in sin-3(tm1276) comparing to in N2. DESeq2, fold change > 2, p-value < 0.01. WBPaper00061203:sin-3(tm1276)_upregulated
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_12h
  Transcripts that showed significantly increased expression in aak-1(tm1944);aak-2(ok524) animals comparing to in N2. DEseq 1.18.0, adjusted p-value < 0.05. WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated
  Transcripts that showed significantly increased expression in alg-1(gk214), comparing to in N2. DESeq2, Fold change > 1.5. WBPaper00051404:alg-1(gk214)_upregulated
  Transcripts that showed significantly decreased expression in sin-3(tm1276) comparing to in N2 at early embryo when there were only 3 -5 eggs in the adult. DESeq2, fold change > 2, adjusted p-value < 0.01 WBPaper00058598:sin-3(tm1276)_downregulated
  Transcripts that showed significantly increased expression in animal with pgph-2 overepxreesion [pgph-2p-pgph-2; myo-2p-mcherry] in glucose excess condition. Genes with anadjusted P-value <= 0.05 found by DESeq2 were assigned as differentially expressed. WBPaper00065926:pgph-2(overepxreesion)_upregulated_glucose
  Transcripts that showed significantly increased expression in xrep-4(lax137). DESeq2. Genes were selected if their p value < 0.01. WBPaper00066062:xrep-4(lax137)_upregulated
Gamma irradiation 100 mGY per hour for 72 hours since L1 larva. Transcripts that showed significantly increased expression after exposure to 100mGy per hour gamma irradiation from L1 to day 1 adult hermaphrodite stage. DESeq2, FDR <= 0.05, log2 fold change >= 0.3 or <= -0.3. WBPaper00058958:100mGy-irradiation-72h_upregulated
  Genes that showed oscillating mRNA expression level throughout the 16 hour time courses from L3 larva to young adult. The following three lines of R code were used to perform the classification: increasing <-2*amplitude-PC1 < -1.7; oscillating <-!increasing & (amplitude > 0.55); flat <-!increasing & !oscillating; Note that the amplitude of a sinusoidal wave corresponds to only half the fold change between trough and peak. WBPaper00044736:oscillating_dev_expression
  Transcripts that showed significantly increased expression in hpk-1(pk1393) comparing to in N2 at adult day 2. DESeq 2, fold change > 2, FDR < 0.05. WBPaper00065581:hpk-1(pk1393)_upregulated

27 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr2871 The mRNAs for lin-42, nhr-23, and nhr-41a exhibit a low-high (peak)-low profile during the L1 intermolt that is precisely reiterated at later stages. The mRNAs for nhr-25a, nhr-25b, and nhr-41b have a high-low-high (peak) expression profile during the L1 stage (and into early L2) that is also reiterated. nhr-25a and nhr-41b also show a downward trend in baseline expression as development proceeds. For all six mRNAs, there are peaks of expression that occur at precise intervals relative to the molt at all larval stages: nhr-25b and nhr-41b peak at the beginning of the intermolt; nhr-23 peaks at mid-intermolt, slightly preceding the intermolt peak in lin-42 expression; and nhr-25a and nhr-41a peak at the time the larval molts occur. Two mRNAs, nhr-6 and nhr-85a, show a more irregular expression pattern with respect to the molt; however, it should be noted that nhr-6 mRNA has consistent relatively lower expression values at the times the molt takes place. nhr-67 also does not display a reiterated, oscillating expression pattern, although two early peaks are observed at the end of the L1 stage and at the beginning of L2.  
    Expr1031669 Tiling arrays expression graphs  
Data observed from, atIs13, atEx32 and atEx35.   Expr970 In comma to 1.5-fold stage embryos, the nhr-25::GFP are expressed in the V cells, P cells and hyp7. Expression also observed in the head and tail hypodermal cells of embryos. The earliest expression is at ~250-300 min post fertilization. atEx32 and atEx35 L1 animals exhibited consistent reporter expression in the hyp7 and P cell nuclei. The P cell expression was very strong at hatching. GFP expression in the P cell and hyp7 decreased during mid-L1, but frequently increased late L1. At hatching no expression in observed in the V cells. In mid L1 the V cells divided and the anterior daughters begin to express GFP as they join the syncytium. Strong expression is also seen in the head and tail hypodermal nuclei of L1 larva. Expression in the hypodermal nuclei of older larva stages is similar to that in the L1. GFP expression decreases markedly in adults. GFP is also observed in other ectodermal cells. In L1, expression is observed in the G2(excretory pore) cell and in a cell tentatively identified as the W neuroblast. In older larva, expression continues in G2 but disappears in the W lineage once the cells divides to generate neurons. Beginning in L2, GFP is expressed in the region around the rectum. Expression is also occasionally observed in the anterior pharynx, in the nuclei with positions consistent with those of the pharyngeal epithelial cells. nuclei
    Expr7322 Click the movie links below for interactive 4D movies of a fluorescence reporter. http://glowormnotes.blogspot.com/2009/11/nhr-25yfp-transcriptional-fusion.html  
    Expr1132 maternal mRNA was evenly distributed in the early embryo. By about 120min, zygotic mRNA expression began in the progeny of E cell. then progressed into the posterior hypodermal precursor cells. Starting from the comma stage, the mRNA expression localized to the hypodermis and intestine, where it continued during early larva stages. Strong expression in the developing and adult germ line. The mRNA appeared in the primordial gonads of L2-L3 larvae and continued to accumulate in the gonadal loops as they expanded in L4. The adult gonad was loaded with transcript. mRNA expressed declined in the rest of the body after L2.  
    Expr11328 NHR-25 was first observed in major epidermal cells of embryo with approximately 200 cells and continuously expressed throughout embryogenesis and larval stages as judged by a combination of cell morphology and position. It was also observed in precursor/blast cells of neuron and epidermis which are called Pnp cells in embryo and in seam cells during early larval stages. Despite its expression in precursors of neurons and epidermal cells, NHR-25 appears not to be expressed in the neurons of larval stage.  
    Expr11461 NHR-25::GFP was evenly distributed prior to the first division in all VPCs, whereas after the first division the pattern became graded: highest in 1 P6.p daughters, lower in 2 P5.p and P7.p daughters, and lowest in 3 P(3,4,8).px. After the third round of cell divisions NHR-25::GFP expression continued in all 22 P(5-7).pxxx cells and remained high during early vulva morphogenesis until it temporarily disappeared by the ''Christmas tree stage''. In wild type animals, NHR-25::GFP was normally expressed in the anchor cell at the time of the first VPC divisions, and subsequently decreased. NHR-25::GFP is expressed in nuclei of seam cells and hyp7.  
    Expr3137 Antibody stained the same nuclei lit by the scm::gfp seam-cell-specific marker at L1 and later stages. A weaker signal was observed in the nuclei of hyp7 cells produced by the first asymmetric division of the V cells. A transgenic nhr-25::gfp construct confirmed that nhr-25 was expressed in the seam cells from L1 until adulthood; consistent with the antibody staining, this construct was less active in the hyp7 daughters. nuclei
Reporter gene fusion type not specified. nhr-25::gfp-C causes early embryonic developing arrest, thus none of the embryos showing GFP expression hatched.   Expr1133 Expression first appeared in the four descendants of the E cell and later in derivatives of AB.p and C blastomeres. The expression continued in the hypodermis but not gut throughout the remainder of the embryogenesis. In L1, the strongest GFP was signals were observed in the seam cells and the pharyngeal and tail hypodermis. Also noted expression in the excretory duct cell. Regardless of NLS, localized to nuclei.
    Expr11078   NHR-25::YFP is expressed in the nuclei and cytoplasm of epidermal cells during morphogenesis.
    Expr3035 An nhr-25::gfp reporter was found to be expressed in the nuclei of many epidermal cells, including the epidermal syncytial cells, the seam cells, and all the Pn.p cells and their progeny. Many cells in the head and tail also showed nhr-25::gfp expression. nhr-25 expression was first observed during embryogenesis around the 100-cell stage and continued to be expressed throughout development in the epidermal and seam cells. In the Pn.p-derived vulval cells, when vulval morphogenesis (including cell division, fusion, and migration) was almost complete during the L4-to-adult transition, nhr-25::gfp expression significantly decreased, whereas in other Pn.p cells that fused with hyp7, nhr-25::gfp expression remained largely unchanged. Expressed in the nuclei of many epidermal cells
    Expr1200106 Data from the TransgeneOme project  
    Expr10510 Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
    Expr10511 Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
    Expr10513 Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
    Expr3975 nhr-25 mRNA is present in the early gonad primordium.  
    Expr11054 nhr-25 transcriptional reporter shows a dynamic expression pattern in which expression in hyp8-11 is intense prior to and at the beginning of tail tip morphogenesis, rapidly shuts off in late L4, and is never on in adult animals.  
    Expr3976 The NHR-25 protein can be detected in the nuclei of Z1 and Z4 as well as in the nuclei of their daughter cells, but not in the Z2 and Z3 precursors of the germline. nuclei
    Expr13677 The 3' nhr-25 enhancer specifically drives GFP expression in approximately 20 hypodermal cells in the head and tail of the worm during larval development.  
    Expr12800 nhr-25 was expressed in V seam cells, in the posterior seam-cell T and its daughters (T.a, T.p), and in the hyp cells of wild-type animals.  
    Expr969 Both nhr-25a and nhr-25b transcripts were detected in all stages. Furthur more, based on detection in serial dilutions relative to ama-1 mRNA levels, both nhr-25 transcripts are more highly expressed in embryos and L1 - L3 stages than in the L4 and adult stages.  
    Expr2014151 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr2032394 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr1148306 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr1014976 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  
    Expr10512 Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
    Expr1200241 Data from the TransgeneOme project  

23 GO Annotation

Annotation Extension Qualifier
  located_in
  part_of
  located_in
  located_in
  involved_in
  involved_in
  involved_in
  involved_in
  enables
  enables
  enables
  enables
  enables
  enables
has_input(WB:WBGene00000039),occurs_in(WBbt:0005753) acts_upstream_of_or_within
  involved_in
  involved_in
  enables
  involved_in
  enables
  enables
  enables
  enables

12 Homologues

Type
orthologue
orthologue
orthologue
least diverged orthologue
least diverged orthologue
least diverged orthologue
orthologue
orthologue
orthologue
least diverged orthologue
orthologue
least diverged orthologue

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00003623 13008493 13013964 1

23 Ontology Annotations

Annotation Extension Qualifier
  located_in
  part_of
  located_in
  located_in
  involved_in
  involved_in
  involved_in
  involved_in
  enables
  enables
  enables
  enables
  enables
  enables
has_input(WB:WBGene00000039),occurs_in(WBbt:0005753) acts_upstream_of_or_within
  involved_in
  involved_in
  enables
  involved_in
  enables
  enables
  enables
  enables

2 Regulates Expr Cluster

Regulated By Treatment Description Algorithm Primary Identifier
  Genes down-regulated following nhr-25(RNAi). Pair-wise significance testing (mutant/RNAi vs. wild-type/vector) was performed using the Bioconductor package limma and p-values were initially corrected for multiple testing using the false discovery rate (FDR) method of Benjamini and Hochberg. Authors defined differential expression as log2(ratio) >= 0.848 with the FDR set to 5%, and p-value <= 0.001. WBPaper00045015:nhr-25(RNAi)_downregulated
  Genes up-regulated following nhr-25(RNAi). Pair-wise significance testing (mutant/RNAi vs. wild-type/vector) was performed using the Bioconductor package limma and p-values were initially corrected for multiple testing using the false discovery rate (FDR) method of Benjamini and Hochberg. Authors defined differential expression as log2(ratio) >= 0.848 with the FDR set to 5%, and p-value <= 0.001. WBPaper00045015:nhr-25(RNAi)_upregulated

1 Sequence

Length
5472

1 Sequence Ontology Term

Identifier Name Description
gene  

13 Strains

WormBase ID
WBStrain00023993
WBStrain00023994
WBStrain00024013
WBStrain00024012
WBStrain00024011
WBStrain00024019
WBStrain00024018
WBStrain00024024
WBStrain00024023
WBStrain00026522
WBStrain00034955
WBStrain00035795
WBStrain00008438

1 Upstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrX_13008420..13008492   73 X: 13008420-13008492 Caenorhabditis elegans