WormMine: Gene WBGene00003289

• WormBase Gene ID: WBGene00003289
• Gene Name: mir-61
• Sequence Name: F55A11.9
• Brief Description: mir-61 encodes a microRNA, a small non-protein coding RNA with a predicted ortholog in C. briggsae; during postembryonic development, mir-61 functions in a feedback loop that promotes the secondary vulval cell fate; in regulating the secondary fate, mir-61 lies downstream of LIN-12/Notch signaling and upstream of vav-1, which encodes a protooncogene that negatively regulates LIN-12 activity and whose expression is, in turn, negatively regulated by mir-61; Northern analyses indicate that mir-61 is expressed constitutively throughout development in normal worms and in glp-4(bn2) adults, while a mir-61::gfp promoter fusion indicates that mir-61 is expressed in the P5.p and P7.p vulval precursor cells and their descendants as well as in the gonad; mir-61 expression is dependent upon LAG-1 binding sites in its promoter region.
• Organism: Caenorhabditis elegans
• Automated Description: Involved in miRNA-mediated post-transcriptional gene silencing and regulation of vulval development. Expressed in several structures, including P5.p hermaphrodite; P5.pa; P5.pp; P7.p hermaphrodite; and P7.pa.
• Biotype: SO:0001265
• Genetic Position: :±
• Length (nt): 97

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00003289

Genomics

2 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:F55A11.9b	F55A11.9b	21	V: 11770076-11770096
Transcript:F55A11.9a	F55A11.9a	22	V: 11770111-11770132

Other

0 CDSs

2 RNAi Result

• WBRNAi00112527
• WBRNAi00112526

11 Allele

• gk963271
• gk963301
• gk964458
• gk964459
• gk964451
• gk963618
• gk964054
• gk964055
• gk334770
• nDf59
• gk248166

1 Chromosome

• WormBase ID: V
• Organism: Caenorhabditis elegans
• Length (nt): 20924180

1 Chromosome Location

• Feature . Primary Identifier: WBGene00003289
• Start: 11770060
• End: 11770156
• Strand: -1

3 Data Sets

• WormBaseAcedbConverter
• GO Annotation data set
• C. elegans genomic annotations (GFF3 Gene)

1 Downstream Intergenic Region

• WormBase ID: intergenic_region_chrV_11770016..11770059
• Length (nt): 44
• Chromosome Location: V: 11770016-11770059
• Organism: Caenorhabditis elegans

27 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
Bacteria: E.faecalis strain OG1RF	Transcripts that showed significantly increased expression after infection by E. faecalis OG1RF.	Ballgown was used to calculate differential expression of genes using FPKM data and to generate tables with fold change and P values. Genes were shortlisted with a cutoff of 2-fold change and P values of less than 0.05.	WBPaper00059754:E.faecalis_OG1RF_upregulated
	Transcripts that showed significantly increased expression in ilc-17.1(syb5296) comparing to in N2 animals at L4 larva stage.	DESeq2, fold change > 2, FDR < 0.05.	WBPaper00066594:ilc-17.1(syb5296)_upregulated
	Potental DAF-12 target genes identified by ChIP-chip analysis performed on strain ALF4 [daf-12	Affymetrix TAS software that computed for each probe estimates of fold enrichment (in linear scale) over hybridization with input DNA. At the same time, TAS calculated for each probe a p-value by applying a Wilcoxon signed rank test. A threshold of 2.5 was selected, which corresponds to probe intensities approximately 2.5 times stronger on the ChIP array than on the Input array. Additional TAS threshold parameters were MinRun=180 bp, MaxGap=300 bp. TAS analysis showed that the selected threshold of 2.5 corresponds approximately to a p-value of 0.01.	WBPaper00040221:DAF-12_target_ALF4
	Genes with small RNAs upregulated in all four meg-3(ax3055) meg-4(ax3052) strains comparing to N2	DESeq2	WBPaper00057162:meg-3_meg-4_upregulated_sRNA
	Transcripts interacting with LAG-1 at L4 larva stage in germline ChIP-Seq analysis.	edgeR fold change >= 2, FDR <= 0.05.	WBPaper00059440:LAG-1_interacting_germline
	Transcripts interacting with LAG-1 at L4 larva stage in whole animal ChIP-Seq analysis.	edgeR fold change >= 2, FDR <= 0.05.	WBPaper00059440:LAG-1_interacting_WholeAnimal
	Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in hypodermis.	Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2.	WBPaper00066447:Day8_vs_Day1_downregulated_hypodermis
	miRNAs that showed significantly decreased expression in glp-4(bn2) at 26 centigrade, comparing to in N2 animals at 26 centigrade.	DESeq2 q-value < 0.05.	WBPaper00049990:glp-4(bn2)_26C_vs_N2_26C_downregulated
	MicroRNAs that showed significantly decreased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_downregulated_miRNA
	miRNAs that showed increased expression in 1 day post L4 adult hermaphrodite eat-2(ad1116) comparing to in N2.	A fold change >= 1.5 with a minimum read count of >= 10 were used to filter the differentially expressed miRNA. The p-value cutoff was set at p <= 0.05 based on Kals Z test statistical.	WBPaper00046156:eat-2(ad1116)_Day1_upregulated
	miRNAs that showed decreased expression in 8 days post L4 adult hermaphrodite eat-2(ad1116) comparing to in N2.	A fold change >= 1.5 with a minimum read count of >= 10 were used to filter the differentially expressed miRNA. The p-value cutoff was set at p <= 0.05 based on Kals Z test statistical.	WBPaper00046156:eat-2(ad1116)_Day8_downregulated
Exposed to 1mg / L PM2.5 from L1 larva to young adult.	MicroRNAs that showed significantly decreased expression after animals were exposed to 1 mg per liter PM2.5 from L1 larva to young adult.	DESeq, cut-off of 2-fold change, p-value < 0.05.	WBPaper00054985:PM2.5_downregulated
	Micro RNAs that showed significantly decreased expression in hrpk-1(tm5522) comparing to in N2 at L4 larva stage.	DESeq, fold change > 2, p-value <= 0.05.	WBPaper00058673:hrpk-1(tm5522)_downregulated_miRNA_L4
	miRNA with increased expression in N2 1-day post L4 adult hermaphrodite comparing to in N2 L4 larva.	DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively.	WBPaper00045316:miRNA_N2_adult_vs_L4_upregulated_adult
	MicroRNAs that showed significantly decreased expression in alg-1(gk214), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-1(gk214)_downregulated_miRNA
	MicroRNAs that showed significantly decreased expression in alg-2(ok304), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-2(ok304)_downregulated_miRNA
	miRNA with decreased expression in prg-1(wm161) comparing to in N2.	DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively.	WBPaper00045316:miRNA_prg-1_downregulated_adult
	MicroRNAs that showed significantly decreased expression in alg-5(tm1163), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(tm1163)_downregulated_miRNA
	Micro RNAs that showed significantly decreased expression in drsh-1(ok369) comparing to in N2.	Fold change > 1.5.	WBPaper00055896:drsh-1(ok369)_downregulated
	MicroRNAs that showed significantly increased expression in alg-5(ram2), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(ram2)_upregulated_miRNA
Bacteria infection: Pseudomonas aeruginosa PA14	miRNAs up regulated by Pseudomonas aeruginosa infection.	N.A.	WBPaper00046303:P.aeruginosa_upregulated_miRNA
	miRNA with increased expression in N2 L1 larva comparing to in N2 embryo.	DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively.	WBPaper00045316:miRNA_N2_L1_vs_embryo_upregulated_adult
	MicroRNAs that showed significantly increased expression in alg-5(tm1163), comparing to in N2.	DESeq2, Fold change > 1.5.	WBPaper00051404:alg-5(tm1163)_upregulated_miRNA
	MicroRNAs that showed significantly decreased expression after animals were exposed to 1 mg per liter multi-walled carbon nanotubes (MWCNTs) from L1 larva to young adult.	DESeq, cut-off of 2-fold change.	WBPaper00045019:MWCNT_downregulated
	miRNA with decreased expression in N2 L4 larva comparing to in N2 L3 larva.	DEGseq and GFOLD were used to analyze miRNAs expression. Authors chose miRNAs which had more than two-fold difference in expression (P < 0.001, q < 0.01 of Storey) from DEGseq, and miRNAs which had more than two-fold difference in expression (GFOLD score > 0 for up-regulation and GFOLD score < 0 for down-regulation) from GFOLD outcomes. Then authors obtained the intersection of up-regulated miRNAs and down-regulated miRNAs for each stage from the chosen miRNAs, respectively.	WBPaper00045316:miRNA_N2_L4_vs_L3_downregulated_adult
	Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in body wall muscle.	Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2.	WBPaper00066447:Day8_vs_Day1_downregulated_BodyWallMuscle
	Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in neuron.	Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2.	WBPaper00066447:Day8_vs_Day1_downregulated_neuron

6 Expression Patterns

• Primary Identifier: Expr8598
• Remark: Picture: Fig. 1B.
• Pattern: A mir-61 transcriptional reporter is specifically expressed in P5.p and P7.p and their daughters.

• Primary Identifier: Expr8597
• Remark: Picture: Fig. 4I, 4J.
• Pattern: mir-61::gfp, driven by 3-kb upstream region, is also expressed in several head neurons and transiently and weekly in some seam cells in L3 and L4 stage worms, in addition to vulva precursor cells and gonadal cells as reported before (see Expr8498).

• Primary Identifier: Expr8464
• Pattern: Expression detected from early embryos to adults. Detected in dnc, two head nerves whose processes run ventral to each other and laterally towards the tip of the head. Also seen in tail nerves and posterior intestine. In L2, expression is also detected in vulval cell precursors.

• Primary Identifier: Expr10364
• Pattern: Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/

• Primary Identifier: Expr10893

• Primary Identifier: Expr2489
• Pattern: Intense signals were detected in embryos, larval stages (L1, L2, L3, L4), adults, and glp-4(bn2) adults.

2 GO Annotation

0 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00003289
• Start: 11770060
• End: 11770156
• Strand: -1

2 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 97

1 Sequence Ontology Term