WormMine: ExpressionPattern Expr1888

• Pattern: The pat-4::gfp transgene is initially expressed weakly throughout postgastrulation embryos. By 420 min postfertilization, PAT-4/ILK::GFP is primarily observed in the body-wall muscle cells, where it colocalizes with the integrin PAT-3 subunit. pat-4::gfp is also transiently expressed in the pharynx during embryogenesis. In adult hermaphrodites, pat-4::gfp expression is observed in body-wall muscle cells, the spermatheca, the vulva muscles, a subset of the mechanosensory neurons (ALM, AVM, PLM, and PVM), the distal tip cells, the uterine muscles, and the anal depressor and anal sphincter muscles.
• Primary Identifier: Expr1888
• Remark: Immunostaining experiments using anti-PAT-4/ILK show an identical localization pattern in wild-type embryos and adults.
• Subcellular Localization: In adult body-wall muscle cells, PAT-4/ILK::YFP colocalizes with a functional PAT-3::CFP integrin subunit in M-lines and dense bodies and at muscle-muscle adherent junctions. PAT-4/ILK::GFP is located near the cell membrane and does not extend very deeply into body-wall muscle cells.

15 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292
a saddle-shaped muscle cell that encircles the intestinal-rectal valve	anal sphincter muscle	lineage name: ABprpppppap	WBbt:0005798
muscle lining of the uterine wall.	uterine muscle		WBbt:0005342
Neuron class of one sensory neuron, anterior ventral microtubule cell, touch receptor.	AVM	lineage name: QR.paa	WBbt:0003832
Neuron class of one neuron, posterior ventral microtuble cell, touch receptor.	PVM	lineage name: QL.paa	WBbt:0004086
Anterior lateral microtubule cell, touch receptor	ALML	lineage name: ABarppaappa	WBbt:0003954
Anterior lateral microtubule cell, touch receptor	ALMR	lineage name: ABarpppappa	WBbt:0003953
Posterior lateral microtubule cell, touch receptor	PLML	lineage name: ABplapappppaa	WBbt:0004104
Posterior lateral microtubule cell, touch receptor	PLMR	lineage name: ABprapappppaa	WBbt:0004103

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003931	pat-4	C29F9.7	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013