WormMine: AnatomyTerm WBbt:0004841

• Definition: Ventral cord motor neurons, innervate dorsal muscles
• Name: DB6
• Primary Identifier: WBbt:0004841
• Synonym: lineage name: ABplppaappp

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABplppaappp	ABplppaappp nucleus		WBbt:0001962

4 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Top 300 transcripts enriched in DB1, DB2, DB3, DB4, DB5, DB6, DB7 according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:DB
	Top 300 transcripts enriched in DA1, DA2, DA3, DA4, DA5, DA6, DA7, DA8, DA9, DB1, DB2, DB3, DB4, DB5, DB6, DB7 according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:DA_DB
	Transcripts enriched in DB according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:DB_enriched
	Transcripts enriched in DB3_7 according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00066550:DB3_7_enriched

43 Expression Patterns

• Primary Identifier: Expr4902
• Remark: Picture: Fig. 3A, B, C, D.
• Pattern: In the transgenic animals carrying crm-1a::gfp reporter, consistent signals were detected in neurons in the ventral nerve cord. By the positions and clustering of the cell body as well as the axonal outgrowth along the ventral nerve cord, crm-1a is found to be expressed in the DA neurons 2 to 7, the DB neurons 3 to 7 and additional neurons in the VA, VB and AS classes along the nerve cord (VNC). Expression was also observed in neurons around the pharynx. In the male tail, gfp signals in the RnA neuronal cells of sensory rays 2 and 4 and the PVR neuron with the entire axonal process along the VNC were also observed.
• Subcellular Localization: Entire axonal process of PVR along the VNC.

• Primary Identifier: Expr15649

• Primary Identifier: Expr15442

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr1842
• Pattern: Staining embryos with this anti-VAB-7 antibody confirmed that VAB-7 is expressed in posterior muscle and epidermal cells. However, a second phase of VAB-7 expression was discovered in embryonic ventral nerve cord (VNC) neurones, beginning at the 1.5-fold stage. At the threefold stage VAB-7 is expressed in nine neuronal nuclei (seven in the VNC and two nuclei in the head), and in the five most posterior epidermal nuclei, which form the hyp8 to hyp11 cells. VAB-7 is expressed in the seven DB class motoneurones; VAB-7 staining is also seen in two additional head neurones that have not been identified. DB neurones express VAB-7 throughout development. In addition, VAB-7 is expressed continuously in the hypodermal syncitium hyp10, and from the L1 stage in four unidentified neurones in the tail. Finally, at the adult stage, VAB-7 is detected in three VC neurones: VC1, VC2 and VC6.
• Subcellular Localization: nuclei

• Primary Identifier: Expr15570

• Primary Identifier: Expr15644

• Primary Identifier: Expr15646

• Primary Identifier: Expr12716

• Primary Identifier: Expr12717

• Primary Identifier: Expr11224
• Remark: At 335 minutes post fertilization.
• Pattern: efn-2 expression showed widespread left-right asymmetry inembryos, both in amphid neurons and in other cells.

• Primary Identifier: Expr15316
• Pattern: acr-5 ORF

• Primary Identifier: Expr15633

• Primary Identifier: Expr12715

• Primary Identifier: Expr15314

11 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes.		late cleavage stage embryo Ce	WBls:0000014

3 Parents

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
Neuron class of seven motoneurons that innervate dorsal muscles and have cell bodies in the ventral cord.	DB neuron		WBbt:0005274
embryonic cell	ABplppaapp		WBbt:0006673