WormMine: AnatomyTerm WBbt:0003989

• Definition: Amphid interneuron
• Name: AIAL
• Primary Identifier: WBbt:0003989
• Synonym: lineage name: ABplppaappa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABplppaappa	ABplppaappa nucleus		WBbt:0001635

3 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Top 300 transcripts enriched in AIAL, AIAR according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:AIA
	Single-cell RNA-Seq cell group 104_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:104_0
	Single-cell RNA-Seq cell group 112_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:112_0

83 Expression Patterns

• Primary Identifier: Expr4270
• Pattern: INS-1::Venus, which was confirmed to be functional, was expressed in several amphid chemosensory neurons, ASE, ASI, and ASJ; AIA interneurons; and several other neurons. Of these expression patterns, the fluorescence signal in AIA was the strongest and most consistent between individual animals.
• Subcellular Localization: In AIA neurons, punctate expression patterns in the cell bodies and processes were observed.

• Primary Identifier: Expr8976
• Remark: Picture: Fig 4E to 4H. npr-5 = Y58G8A.4 in this paper.
• Pattern: The npr-5p::RFP transgene was expressed in a subset of amphid neurons (ADF, ASE, ASG, ASI, ASJ, ASK, AWA, AWB) in the inner labial neuron IL2, in the interneurons AIA and AUA, and in the phasmids (PHA, PHB). Outside the nervous system, npr-5p::RFP was expressed in head, neck, and body muscles throughout larval and adult development.

• Primary Identifier: Marker52
• Remark: Picture: N.A. Reporter gene fusion type not specified.
• Pattern: Expressed in AIY and AIA neurons.

• Primary Identifier: Expr15388

• Primary Identifier: Expr11753
• Remark: genomic

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr15410
• Pattern: sra-11(pB)

• Primary Identifier: Expr9242
• Pattern: SCD-2 is expressed in many neurons including AIA interneurons.

• Primary Identifier: Expr3136
• Pattern: C33G8.5 showed medium to strong expression in scattered nonchemosensory neurons.5

• Primary Identifier: Expr12179
• Reporter Gene: Reporter fusion construct not specified.
• Pattern: Reporter genes for npr-11 are expressed in two postsynaptic targets of AWC, the AIA and AIY interneurons, and in other neurons.

• Primary Identifier: Expr7808
• Remark: Picture: Figure 7.
• Pattern: A highly reproducible pattern of DKF-1 expression was observed as animals matured from embryo to adult. Intense fluorescence signals corresponding to DKF-1GFP revealed robust kinase accumulation in both (a) a region bounded by the anterior and posterior bulbs of the pharynx and (b) a tail area that contains lumbar, dorsorectal and pre-anal ganglia. Specifically, DKF-1 is differentially enriched in a cluster of cells that are immediately adjacent to the posterior pharyngeal bulb. Strong signals also emanate from cells positioned along the lateral surface of this bulb in animals carrying the dkf-1P::DKF-1GFP transgene. At the anterior pharyngeal bulb, DKF-1 accumulates selectively in bodies and in very thin processes (dendrites and axons) of two neurons. Nearly all cells expressing DKF-1 appear to be neurons. Two fluorescent cells with similar sizes and locations (at the anterior edge of the isthmusposterior bulb) may be M2 motor neurons. The location of the more posterior fluorescent neuron approximates the position of the cell body of an NSM neuron. DKF-1 also accumulates in a cell resembling I1. Other candidate DKF-1-enriched cells in the pharyngeal region include: the AWB, ADL, and ADF chemosensory neurons; and AVB and AIA interneurons.n C. elegans tail, DKF-1GFP expression is differentially elevated in neurons located within the dense neuropile of several tail ganglia. The pattern of fluorescence reveals that cell bodies and/or processes of phasmid neurons (PHA, PHB, and PHC), interneurons (PVC, DVA, DVB, PVQ, PVT) and motor neurons (VD13, DD6, VA12) are candidate sites for accumulation of DKF-1 protein.
• Subcellular Localization: Expressed in neuronal cell bodies and/or processes.

• Primary Identifier: Expr10059
• Pattern: As previously reported, ins-1::GFP fluorescence was observed in many head neurons, including ADF, AIA, AIM, ASE, ASG, ASH, ASI, ASJ, AWA, BAG, and NSM, and was also observed in the intestine, hypodermis, and vulval muscle.

• Primary Identifier: Expr15364

• Primary Identifier: Expr538
• Remark: Integrated strain PS1445 [dpy-20(e1362); him-5(e1490); syIs7(gpa-2::lacZ)].
• Pattern: Expressed in PVT, phasmids, AIA cells, mu_sph, M1, M5, and I5. The lacZ construct, but not a GFP construct (see Expression pattern 539), was expressed in AWC cells.

• Primary Identifier: Expr12716

• Primary Identifier: Expr12717

• Primary Identifier: Expr15327
• Remark: 3.7 kb

• Primary Identifier: Expr15329
• Remark: (3.3-2.6k)

11 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes.		late cleavage stage embryo Ce	WBls:0000014

5 Parents

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
ventral ganglion, anterior part.	ventral ganglion (ant)		WBbt:0005266
cell that exists in and is part of an embryo.	embryonic cell		WBbt:0007028
embryonic cell	ABplppaapp		WBbt:0006673
Neuron class of two interneurons with cell bodies situated in the ventral ganglion.	AIA		WBbt:0006812