WormMine: ExpressionPattern Expr11136

• Pattern: hda-1 is broadly expressed throughout development. The earliest expression was detected in gastrulating embryos. The larvae exhibited GFP expression in several neuronal and epidermal cells, primarily in the anterior ganglion and ventral hypodermal regions. Expression persisted in many cells in later larval and adult stages (data not shown). In the vulva, hda-1::gfp expression was first detected in the progeny of P(5-7).p in mid-L3 animals. At this stage, GFP fluorescence was absent in other VPC lineages (P3.p, P4.p and P8.p) (data not shown). By the L4 stage, almost all vulval cell types were observed fluorescing, with presumptive vulA, vulB1, vulB2, and vulD cells being the brightest. GFP fluorescence in vulval cells was mostly absent beyond the late-L4 stage, suggesting that hda-1 may not be needed in vulval cells at later stages of development. The broad expression of hda-1 is in consistent with the involvement of the gene in multiple developmental processes. This multifaceted role for hda-1in C. elegans appears to be conserved in C. briggsae, because Cbr-hda-1::gfp is expressed in a similar manner. hda-1::gfp expression was also observed in the AC in L3 animals that persisted until the early L4 stage (data not shown). No expression was observed in pi cells or their progeny at any developmental stage.
• Primary Identifier: Expr11136
• Remark: Expression of gfp in sEx13706 animals is directed by a 2.8 kb hda-1 regulatory region that includes the open reading frames and potential cis-regulatory elements(enhancers) of two other hda-1 upstream genes (ril-1 and C53A5.2). The other hda-1::gfp transgenic strain (bhEx72), generated in this study, contains a much smaller 5'upstream region of hda-1 (approximately 1.0 kb, pGLC44) and excludes the two genes mentioned above. The analysis of GFP fluorescence in sEx13706 and bhEx72 animals revealed a similar pattern, although the fluorescence in sEx13706 was much brighter.

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522
adult vulval toroid cell, B1, formed by fusion of precursors of lineage names P5.papa and P7.ppap.	vulB1		WBbt:0006763
adult vulval toroid cell, B2.	vulB2		WBbt:0006764
adult vulval toroid cell, D, formed by fusion of precursors of lineage names P5.ppp and P7.paa.	vulD		WBbt:0006766
adult vulval toroid cell, A, formed by fusion of precursors of lineage names P5.paaa, P5.paap, P7.pppa and P7.pppp	vulA		WBbt:0006762
ganglion anterior to the nerve ring	anterior ganglion		WBbt:0005375

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001834	hda-1	C53A5.3	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010