WormMine: ExpressionPattern Expr1837

• Pattern: Expression of eff-1p::gfp was silent through the first third of embryogenesis, first appearing about 230 min after first cleavage in a subset of epidermal precursor cells. Over the next 3 hr, these and additional fluorescent cells were observed to migrate over the ventral and dorsal surfaces of the embryo, and the majority of GFP-positive cells fused to form the hyp6 and hyp7 syncytia. As elongation progressed, GFP was also expressed in a pair of cells that fused to form the binucleate "tail spike" . After hatching, eff-1p::gfp expression persisted in large epidermal syncytia through adulthood. Mononucleated epidermal cells-including the seam cells and the VPCs-remained nonfluorescent until shortly before undergoing larval fusion events. More specifically, GFP was seen in (1) nonstem daughters of the seam cells shortly before they fused into hyp7; (2) vulval cells invaginating to form toroids during morphogenesis; and (3) the rays and fan of the adult male tail. Expression was also seen in nonepidermal organs known to contain syncytia, including the pharynx and uterus. Interestingly, a few cells that express eff-1p::gfp have never been observed to fuse, such as some ventral epidermal precursors in the embryo and several neurons.
• Primary Identifier: Expr1837

69 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
Post-embryonic cell of pedigree V6L.pppapaaa, male sensory rays, neuron, striated rootlet.	R4AL	lineage name: V6L.pppapaaa	WBbt:0004030
Post-embryonic cell of pedigree V6R.pppapaaa, male sensory rays, neuron, striated rootlet.	R4AR	lineage name: V6R.pppapaaa	WBbt:0004029
Post-embryonic cell of pedigree V6L.papapaaa, male sensory rays, neuron, striated rootlet.	R2AL	lineage name: R2L.aaa	WBbt:0004042
Post-embryonic cell of pedigree V6R.papapaaa, male sensory rays, neuron, striated rootlet.	R2AR	lineage name: R2R.aaa	WBbt:0004041
Cylindrical hypodermal syncytium in head	hyp6	lineage name: ABarpaapaa	WBbt:0004679
Post-embryonic cell of pedigree V6L.papppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R3BL	lineage name: V6L.papppapa	WBbt:0004034
Post-embryonic cell of pedigree V6R.papppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R3BR	lineage name: V6R.papppapa	WBbt:0004033
Post-embryonic cell of pedigree TL.appaaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R8BL	lineage name: TL.appaaapa	WBbt:0003990
Male sensory rays, neuron, darkly staining tip, open to outside	R8BR	lineage name: TR.appaaapa	WBbt:0003988
Post-embryonic cell of pedigree V6L.pppapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R4BL	lineage name: V6L.pppapapa	WBbt:0004028
Post-embryonic cell of pedigree V6R.pppapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R4BR	lineage name: V6R.pppapapa	WBbt:0004027
Post-embryonic cell of pedigree V6L.papapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R2BL	lineage name: V6L.papapapa	WBbt:0004040
Post-embryonic cell of pedigree V6R.papapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R2BR	lineage name: V6R.papapapa	WBbt:0004039
Post-embryonic cell of pedigree V5L.pppppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R1BL	lineage name: V5L.pppppapa	WBbt:0004048
Post-embryonic cell of pedigree V5R.pppppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R1BR	lineage name: V5R.pppppapa	WBbt:0004046
Post-embryonic cell of pedigree TL.appapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R9BL	lineage name: TL.appapapa	WBbt:0003968
Post-embryonic cell of pedigree TL.apappapa, male sensory rays, neuron, darkly staining tip, open to outside.	R7BL	lineage name: TL.apappapa	WBbt:0004001
Male sensory rays, neuron, darkly staining tip, open to outside	R7BR	lineage name: TR.apappapa	WBbt:0004000
Male sensory rays, neuron, darkly staining tip, open to outside	R9BR	lineage name: TR.appapapa	WBbt:0003966
Post-embryonic cell of pedigree V6L.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BL	lineage name: V6L.ppppaapa	WBbt:0004022
Post-embryonic cell of pedigree V6R.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BR	lineage name: V6R.ppppaapa	WBbt:0004021
Post-embryonic cell of pedigree V6L.papppapp, male sensory rays, structural cell.	R3stL	lineage name: V6L.papppapp	WBbt:0004032
Post-embryonic cell of pedigree V6R.papppapp, male sensory rays, structural cell.	R3stR	lineage name: V6R.papppapp	WBbt:0004031
Post-embryonic cell of pedigree V6L.pppapapp, male sensory rays, structural cell.	R4stL	lineage name: V6L.pppapapp	WBbt:0004026
Post-embryonic cell of pedigree V6R.pppapapp, male sensory rays, structural cell.	R4stR	lineage name: V6R.pppapapp	WBbt:0004025
Post-embryonic cell of pedigree V5L.pppppapp, male sensory rays, structural cell.	R1stL	lineage name: V5L.pppppapp	WBbt:0004044
Post-embryonic cell of pedigree V5R.pppppapp, male sensory rays, structural cell.	R1stR	lineage name: V5R.pppppapp	WBbt:0004043

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001159	eff-1	C26D10.5	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010