WormMine: ExpressionPattern Expr2633

• Pattern: GFP was expressed in several head neurons located on the sides of the pharynx anterior bulb, around the isthmus and on the sides of the pharynx posterior bulb. Some neuronal processes were fluorescent in the ventral cord down to the tail ganglion, where several neurons expressed GFP as well as several nuclei of hypodermal cells at the tip of the tail. Histochemical staining of mutant ace-1 revealed a strong positive reaction in the nerve ring (NR), the pharyngo-intestinal valve (PIV), the tail ganglion and in the ventral nerve cord, with possibly stained motoneurons which were not labelled with GFP. Head neuron staining allowed identification of the inner labial neurons (IL). AWC and possibly AWB were identified. Cell bodies of these four chemosensory neurons were located below the nerve ring. Other head neurons cannot be identified because of the lack of clear labelling of their processes. The three muscle cells of the pharynx isthmus (pm5) were also fluorescent. In the tail region, three neurons were tentatively identified as PVC and PVQ and PDA. At the tail tip, five fluorescent nuclei of hypodermal cells (hyp 8, hyp 9, binucleated hyp 10 and hyp 11) were identified. ace-2 fluorescence was detected before hatching in the anterior part of the embryo as early as the one-fold stage. The full expression pattern of the adult was already established in L1 larvae.
• Primary Identifier: Expr2633
• Remark: Reporter gene fusion type not specified.

18 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A group of six equivalent cells forms a tightly constructed `valve` that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine.	pharyngeal-intestinal valve	cardia	WBbt:0005767
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
Tail ventral hypodermis	hyp10	lineage name: ABplppppppp	WBbt:0004378
Tail ventral hypodermis, 8th hypodermis cell	hyp8		WBbt:0004381
Tail ventral hypodermis, 9th hypodermis cell	hyp9		WBbt:0004380
Tail dorsal hypodermis	hyp11	lineage name: Cpappv	WBbt:0004377
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBR	lineage name: ABpraaappap	WBbt:0003828
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBL	lineage name: ABalpppppap	WBbt:0003829
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCL	lineage name: ABplpppaapaa	WBbt:0004094
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCR	lineage name: ABprpppaapaa	WBbt:0004092
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWCL	lineage name: ABplpaaaaap	WBbt:0003827
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWCR	lineage name: ABprpaaaaap	WBbt:0003826
Interneuron, projects along ventral cord to ring	PVQL	lineage name: ABplapppaaa	WBbt:0004076
Interneuron, projects along ventral cord to ring	PVQR	lineage name: ABprapppaaa	WBbt:0004074
fifth pharyngeal muscle cell layer	pm5	M5	WBbt:0003737
neuron type, member of a set of 12 neurons of the inner labial sensilla.	inner labial neuron	IL neuron	WBbt:0005117
Neuron class of one motor neuron, process in dorsal cord, same as Y cell in hermaphrodite, Y.a in male.	PDA		WBbt:0004386

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000036	ace-2	Y44E3A.2	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019