WormMine: ExpressionPattern Expr3511

• Primary Identifier: Expr3511
• Subcellular Localization: In the amphid, DAF-6::GFP fusion protein expression usually persisted only up to the L1 larval stage, and the protein localized to the region of the amphid channel formed by the sheath and socket cells. DAF-6::GFP also localized to the luminal surfaces of tubes generated by other cells expressing daf-6. As in the amphid, expression in the phasmid sheath and socket cells usually did not persist beyond the L1 larval stage. Expression in vulval cells was usually restricted to the L4 larval stage, after the cells were generated and during or shortly after the vulval lumen was generated. Expression in the rectum and excretory system was observed throughout embryogenesis and larval development, but usually not during adulthood. DAF-6::GFP protein was detected in punctate structures within the cytoplasm of expressing cells. This localization was best seen in the vulva and in the excretory canal cell. Thus, DAF-6 may localize to vesicles as well.

21 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
gland cell of the secretory-excretory system, sends processes to ring, opens into excretory duct.	excretory gland cell	exc gl	WBbt:0005776
Amphid socket cell, left	AMsoL	lineage name: ABplpaapapa	WBbt:0003931
Amphid socket cell, right	AMsoR	lineage name: ABprpaapapa	WBbt:0003929
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775
The extracellular sinus formed by the excretory duct and pore cells.	Excretory duct		WBbt:0005777
Embryonic cell of pedigree ABplaapaapp, amphid sheath cell, left.	AMshL	ABplaapaapp	WBbt:0003935
Embryonic cell of pedigree ABpraapaapp, amphid sheath cell, right.	AMshR	ABpraapaapp	WBbt:0003933
Post-embryonic cell of pedigree TR.paa. phasmid sockets.	PHso1R	lineage name: TR.paa	WBbt:0004110
Post-embryonic cell of pedigree TL.pap, phasmid sockets.	PHso2L	lineage name: TL.pap	WBbt:0004108
Post-embryonic cell of pedigree TR.pap, phasmid sockets.	PHso2R	lineage name: TR.pap	WBbt:0004106
Post-embryonic cell of pedigree TL.paa, phasmid sockets.	PHso1L	lineage name: TL.paa	WBbt:0004112
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar.	vulF		WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr.	vulE		WBbt:0006767
The outlet of the excretory sinus to the exterior environment through the cuticle, on the ventral side of the head, just behind the nerve ring.	excretory pore	pore	WBbt:0005778
Rectal cell, blast cell in male	F cell	lineage name: ABplppppapp	WBbt:0004799
Rectal cell, postembryonic blast cell in male	U cell	lineage name: ABplppppapa	WBbt:0004942
Rectal cell, postembryonic blast cell	K cell	lineage name: ABplpapppaa	WBbt:0004499
Phasmid sheath	PHshR	lineage name: ABprpppapaa	WBbt:0004114
Phasmid sheath	PHshL	lineage name: ABplpppapaa	WBbt:0004116
Rectal cell	K' cell	lineage name: ABplpapppap	WBbt:0004497

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000902	daf-6	F31F6.5	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003