WormMine: ExpressionPattern Expr2628

• Pattern: Expression was detected in a few nuclei in the head as the embryo reached morphogenesis stage at ~300 minutes of development. Expression was identified in more and more nuclei as development proceeded, so that by the 1 1/2-fold stage a large number of neuronal cells in the head and a few cells in the pharynx expressed zag-1::YFP. At this stage expression was also prominent in motorneurons in the ventral cord and in neurons in tail ganglia. Expression was maintained during the 3-fold stage, but reduced to undetectable levels in most cells before hatching, when only a few cells in the head still expressed zag-1::YFP. In the L1/L2 stage expression was detected transiently in postembryonic motorneurons. Expression was maintained in a few head neurons throughout the entire life cycle. Pzag-1::YFP construct was expressed predominantly in neurons in head and tail ganglia, starting approximately midway through embryogenesis. In some of these neurons expression was maintained throughout development. Additional expression was found consistently in the intestinal and anal depressor muscles during all life stages as well as occasionally in body-wall muscles during embryogenesis.
• Primary Identifier: Expr2628
• Subcellular Localization: zag-1::YFP signal was detected only in the nuclei of cells.

10 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
intestinal muscle cell, attach to intestine and body wall anterior to anus	intestinal muscle	stomato-intestinal muscle	WBbt:0005796
fifth pharyngeal muscle cell layer	pm5	M5	WBbt:0003737
fourth pharyngeal muscle cell layer	pm4	M4	WBbt:0003739
Neuron class of one pharyngeal motorneuron.	M4 neuron	lineage name: MSpaaaaaa	WBbt:0004467

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006970	zag-1	F28F9.1	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013